Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 8.3, Problem 1CC
Retrieve, Infer, Apply
1. What are depth filters and membrane filters, and how are they used to sterilize liquids?
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Chapter 8 Solutions
Prescott's Microbiology
Ch. 8.1 - MICRO INQUIRY Which types of agents can be used...Ch. 8.1 - Retrieve, Infer, Apply 1. Define the following...Ch. 8.1 - Retrieve, Infer, Apply 2. What is the difference...Ch. 8.2 - MICRO INQUIRY Examine graph (a). How long would it...Ch. 8.3 - MICRO INQUIRY How might one verify that filtration...Ch. 8.3 - Retrieve, Infer, Apply 1. What are depth filters...Ch. 8.3 - Retrieve, Infer, Apply 2. Describe the operation...Ch. 8.4 - Retrieve, Infer, Apply 1. Describe how an...Ch. 8.4 - Retrieve, Infer, Apply 2. In the past, spoiled...Ch. 8.4 - Prob. 3CC
Ch. 8.4 - Retrieve, Infer, Apply 2. What is the correlation...Ch. 8.5 - MICRO INQUIRY Why is it important that all of...Ch. 8.5 - MICRO INQUIRY Why are cross-linking agents such as...Ch. 8.5 - Retrieve, Infer, Apply 1. Why are most...Ch. 8.5 - Retrieve, Infer, Apply Construct a table that...Ch. 8.5 - Retrieve, Infer, Apply 3. Which disinfectants or...Ch. 8.5 - Retrieve, Infer, Apply 4. How do phenolic agents...Ch. 8.5 - Retrieve, Infer, Apply 5. Which physical or...Ch. 8.6 - Retrieve, Infer, Apply 1. Briefly explain how the...Ch. 8.6 - Retrieve, Infer, Apply How does being in a biofilm...Ch. 8.6 - Retrieve, Infer, Apply Suppose hospital custodians...Ch. 8.6 - Retrieve, Infer, Apply Briefly describe the phenol...Ch. 8.6 - Retrieve, Infer, Apply Why might it be necessary...Ch. 8.7 - Retrieve, Infer, Apply How would you explain to a...Ch. 8.7 - Retrieve, Infer, Apply Propose the use of specific...Ch. 8 - Prob. 1RCCh. 8 - Throughout history, spices have been used as...Ch. 8 - Design an experiment to determine whether an...Ch. 8 - Suppose you tested the effectiveness of...Ch. 8 - Prob. 4AL
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- Title: Spoilage of coconut water Aim: To assess the diversity of psychrotrophic bacteria causing spoilage in coconut water. How might incubation of plates at 37°C influence the results of the quantitative dilution and plating of the coconut water samples?arrow_forwardPlease answer fast Dilution Problem. A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated. Growth Problem.A culture with approximately 2x103 cells/mL were incubated. After 3 hours, the number of cells had increased to 3x105. a) How long was the generation time in minutes?b) How many generations have occurredarrow_forwardWhen preparing samples for SDS-PAGE, what is the purpose of boiling the samples at 95°C?arrow_forward
- Why should agar media be completely dissolved before they are dispensed in tubes and plates? What are the bases for pegging the temperature at 1210C for 15-30 minutes during moist heat sterilization and 1800C for two (2) hours using dry heat sterilization? Can you sterilize culture media using dry heat sterilization? Why is that so? You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms? Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?arrow_forwardselective media and explain its applications using specific prodving bacterial examples.arrow_forwardi. Illustrate the dilution series used and label the final dilution of each dilution.arrow_forward
- Isolating a pure culture from a mix sample is typically done on solid agar but can be performed using a liquid media. How can you isolate a pure culture from a mixed culture in a liquid medium (broth, not agar)?arrow_forwardTopic: Yeast cells (dalmau plates) What are the principles of PDA, AcA, and MA plates in media preparation? please explain allarrow_forwardHow could calculate this serial dilutions probelms for microbiology and how do can I know what tube has "10^(_)" when making dilutions?arrow_forward
- How does gel filtration work? What are the applications of analytical versus preparative versions of the method ?arrow_forwardProvide three reasons why the use of aseptic technique is essential when handling microbial cultures in the laboratory.arrow_forwardList 5 other methods of sterilization and describe the principle/s and when each method is used. What are the requirements to support microbial growth?arrow_forward
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