Microbiology: Principles and Explorations
10th Edition
ISBN: 9781119390114
Author: Black
Publisher: WILEY
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Chapter 7, Problem 1.3SC
Summary Introduction
To calculate: The average number of bacteria/ml present in the broth that is diluted to 1:1000 dilution that initially contains 37,000 bacteria/ml.
Introduction: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it contains the rigid cell wall. It is classified into different types based on the structure namely coccus (spherical shaped), Bacillus (rod-shaped), filamentous, spirilla (spiral-shaped), appendaged and pleomorphic bacteria. The structure of the bacteria can be viewed under the microscope by using the staining methods.
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Chapter 7 Solutions
Microbiology: Principles and Explorations
Ch. 7 - What are the differences between the lag phase and...Ch. 7 - How does logarithmic rate of increase differ from...Ch. 7 - Prob. 1.3SCCh. 7 - Why does a direct microscopic count of bacteria...Ch. 7 - What does the ending -phile mean? Distinguish...Ch. 7 - What enzymes do most obligate anaerobes lack? How...Ch. 7 - Prob. 2.3SCCh. 7 - Prob. 3.1SCCh. 7 - Distinguish between the various kinds of media:...Ch. 7 - What is the purpose of a stock culture? Why is it...
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- If there is 50 bacterial colonies on a 1:1000 dilution streak plate. How many cfu/mL are there?arrow_forwardYou were instructed to add 1.0 mL out of 4.0 mL of an undiluted sample to 99 mL of sterile diluent. Instead, you add the entire 4.0 mL to the 99 mL. A.) What was your intended dilution factor? B.) What was your actual dilution factor?arrow_forwardIn this experiment, a culture was serially diluted to the concentrations below. Each plate was plated with 0.25mL of the dilution. Using the most diluted plate, what is the correct concentration of the original culture? A) 2.0 X 10^-3 cells/mL B) 2.0 X 10^5 cells/mL C) 4.0 X 10^5 cells/mL D) 5.0 X 10^4 cells/mLarrow_forward
- Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL bacterial concentration.arrow_forwardYou do two 1:5 serial dilutions on an actively growing bacterial culture broth. Doing a plate count of your final serial dilution gives you 30 CFU/mL. What is your original concentration of bacterial culture broth?arrow_forwardWith a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable number of cells in original culture (CFU/ml)?arrow_forward
- You have found that the D-value (decimal reduction value) of an antimicrobial agent to be 4 minutes when the agent was exposed to a bacterial culture having an initial total viable cel1 count of 10° CFU/mL. After 4 minutes upon addition of the antimicrobial agent, what would have been the total viable count of the culture in this experiment? A) O 10° CFU/mL B) O 10$ CFU/mL C) O 10° CFU/mL D) O 10' CFU/mLarrow_forwardYou aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. You then repeat this procedure two more times. What is the final dilution factor of this serial dilution? O 1) 10-4 O 2) 102 O 3) 10-6 4) 10-2 O 5) 104arrow_forwardA bacterial culture has a concentration of 3.2 x 108 cells /mL. You dilute this culture as follows: 1/50, then 10-3 and finally 1/20. If you then plate 0.2 mL of the final dilution, how many CFU would you expect following incubation?arrow_forward
- What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter culture media for a concentration of 6.5 x 106 cells/mL?arrow_forwardBelow is a set of data for a serial dilution/plate count experiment. Based on this data what is the calculated concentration of bacteria in CFU/mL in the original culture? The OD600 of a bacterial culture that's been diluted in half is 0.842. What is the estimated population of cells in the original undiluted culture? asaparrow_forwardDesign a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL bacterial concentration. show solutionarrow_forward
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