Laboratory Manual for Holes Human Anatomy & Physiology Fetal Pig Version
14th Edition
ISBN: 9781259295645
Author: Terry R. Martin
Publisher: McGraw-Hill Education
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Chapter 5, Problem 4.12A
Summary Introduction
Toexplain: The disadvantage of slicing the cells into thin slices before observing under a transmission electron microscope.
Introduction:
The transmission electron microscope is a technique of microscopy that uses a beam of electrons instead of light to get the detailed image of the specimen. The electron beam is trajected in a vacuum chamber towards the specimen to avoid and hindrance due to air particles.
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Students have asked these similar questions
Which of the following microscopes typically requires the use of vital dyes (like methylene blue) to visualize large subcellular structures in a living cell (like the nucleolus or the mitochondrion)?
the scanning/tunneling electron microscope (STEM), with good resolution up to about 100,000,000x
the transmission electron microscope (TEM), with good resolution up to about 100,000x
the scanning electron microscope (SEM), with good resolution up to about 1,000,000x
the compound light microscope, with good resolution up to about 1,500x
all of the above microscopes would be equally useful in visualizing the interior of organelles
You are interested in examining the internal structures of a living cell, what type of
microscopy will you use?
a) phase-contrast microscopy
b) bright-field microscopy
c) transmission electron microscopy
d) scanning electron microscopy
What are the advantages of using many varied methods to connect structure and function in cells? Give some examples.
Chapter 5 Solutions
Laboratory Manual for Holes Human Anatomy & Physiology Fetal Pig Version
Ch. 5 - Match the cellular components in column A with the...Ch. 5 - Prob. 2.1ACh. 5 - Prob. 2.2ACh. 5 - Are the stained cheek cells nearly the same size...Ch. 5 - What do the various types of cells in these...Ch. 5 - What are the main differences you observed among...Ch. 5 - Electron micrographs represent extremely thin...Ch. 5 - Electron micrographs represent extremely thin...Ch. 5 - Electron micrographs represent extremely thin...Ch. 5 - Electron micrographs represent extremely thin...
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- Scanning electron microscope can be used for () A. Observe images of different sections of cells B. Observe living cells C. Quantitative analysis of chemical composition in cells D. Observation of cell surface stereomorphology E. Observation of cellular activityarrow_forwardIf you made a plant cell drawing and that drawing measured 100 mm, what is the plant cell's magnification that measured 20 µm under the microscope? Answer: You were interested to see a human cell under the microscope, so you went to the lab to look for a dye to stain human cells you looked for a stain called. Answer:arrow_forwardAfter investigation of a specimen under light microscope and electron microscope, numerous amounts of spherical shaped particles were seen in the electron microscope while this type of structure was not visible under the light microscope. Further characterization revealed that these particles lack cellular organelles (like the nucleus, mitochondria…). Considering the above circumstance, answer the following questions. (A) What do you think would be the size of the specimen? (B) What do you suspect these particles are most likely to be? (C) Would you define these particles as living? Why/why not-justify your answer brieflyarrow_forward
- Define how to grow cells in culture.arrow_forwardWhy is the microscope important to the study of biology?arrow_forwardThe following fluorescence microscopy shows several cells. What part of the cell is labelled by the fluorescence here? b The Cytosol The Endoplasmic Reticulum The nucleolus The nucleus The actin cytoskeletonarrow_forward
- How do microscopy and biochemistry complement each other to reveal cell structure and function?arrow_forwardIn the micrograph below, you are seeing a collection of cells that have been stained with fluorescent dyes. One of the dyes appears green, and the other dye appears blue (when viewed with a fluorescence microscope). Based on the appearance of these stained cells, which of the following cell structures must the GREEN dye be staining? Endoplasmic reticulum Cytoskeleton Flagella Mitochondria Nucleus ..arrow_forwardExplain the rationale behind the separation of different cellular components using centrifuge.arrow_forward
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