Laboratory Manual for Holes Human Anatomy & Physiology Fetal Pig Version
14th Edition
ISBN: 9781259295645
Author: Terry R. Martin
Publisher: McGraw-Hill Education
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Chapter 5, Problem 2.2A
Summary Introduction
To analyze:
The advantages of staining cells.
Introduction:
Staining is the technique in biology, which increases the visibility at the microscopic level. Dyes and stains are commonly used by the biologist for certain observations. The dye is used for the general-purpose, whereas the stain is used for biological purposes.
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Chapter 5 Solutions
Laboratory Manual for Holes Human Anatomy & Physiology Fetal Pig Version
Ch. 5 - Match the cellular components in column A with the...Ch. 5 - Prob. 2.1ACh. 5 - Prob. 2.2ACh. 5 - Are the stained cheek cells nearly the same size...Ch. 5 - What do the various types of cells in these...Ch. 5 - What are the main differences you observed among...Ch. 5 - Electron micrographs represent extremely thin...Ch. 5 - Electron micrographs represent extremely thin...Ch. 5 - Electron micrographs represent extremely thin...Ch. 5 - Electron micrographs represent extremely thin...
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- Simple Stain What is the difference between simple stain and complex stain? Describe the color of an S. aureus after performing simple stain with Safranin Red. Why? What do you conclude after performing simple stain and observing “Purple cells longer than they are widearrow_forwardStaining of cells is often performed to enhance images acquired by brightfield microscopy. Phasecontrast microscopy does not require cell staining. Why is this advantageous?arrow_forwardExplain the importance of steam during the acid-fast staining procedure. Discuss the advantages of differential staining procedures over the simple staining techniques. List and briefly describe the functions/purpose of each of the different stains used in acid fast staining.arrow_forward
- Give three situations where negative staing procedure would be used?arrow_forwardThe benefits of the negative stain include: Mark all that apply: 1. No shrinkage or distortion of cells due to no heat fixing 2. Because the cells don't take up stain they don't get shrunk or distorted 3. One can more accurately determine the cell size and shape because there is no shrinkage or distortion of cells 4. Negatively charged dyes are safer to work with than positively charged dyes 5. One can more accurately determine cell size and shape because the cells stand out against the backgroundarrow_forward1) What happens to the cells when you heat-fix them, and what are the two goals or purposes of heat-fixing cells prior to staining them? 2) How do direct stains work, that is, how or why do direct stains stain? 3) Why do cells appear to be white or colorless when you use indirect stains?arrow_forward
- What is an example of a negative stain?arrow_forwardDuring staining, the first step is to dewax the slide. What is the purpose of the step? What is the effect of insufficient dewaxing?arrow_forwarda: What are the components of a Romanowsky stain? b: What is the optimum pH for staining with Giemsa. c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.arrow_forward
- Explain in detail simple and differential staining and also improving and adjusting contrast in light microscopy.arrow_forwardThe benefits of the negative stain include: Mark all that apply: O No shrinkage or distortion of cells due to no heat or methanol fixing Because the cells don't take up stain they don't get shrunk or distorted O One can more accurately determine cell size and shape because the cells stand out against the background One can more accurately determine the cell size and shape because there is no shrinkage or distortion of cells O Negatively charged dyes are safer to work with than positively charged dyes IMicrosoft B 11:32 Aarrow_forward1) What is embedding? 2) What is the most common embedding medium for formalin-fixed tissues? 3) What is H& E staining and what does it stain?arrow_forward
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