Microbiology With Diseases By Taxonomy (6th Edition)
6th Edition
ISBN: 9780134832302
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 4, Problem 1CM
Using the following terms, fill in the following concept map that describes
Decolorizer
Ethanol and acetone
Gram-negative bacteria
Gram-positive bacteria
Iodine
Primary stain
Purple
Safranin
Thin peptidoglycan layer
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Search for the following bacteria and complete the table below.
Bacterium
Stain
Description or sketch of cells with the specified feature
Flavobacterium capsulatum
Capsule stain
Corynebacterium diphtheriae
Methylene blue (metachromatic granules)
Mycobacterium tuberculosis
Acid fast stain
You must write up your OWN dichotomous key for all the possible unknown organisms listed below . The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures .
Possible Organisms
Alcaligenes faecalis
Enterobacter aerogenes
Enterococcus faecalis
Escherichia coli
Proteus vulgaris
Pseudomonas aeruginosa
Salmonella arizoniae
Staphylococcus aureus
Staphylococcus epidermidis
Staphylococcus saprophyticus
Streptococcusbovis
Streptococcus pyogenes
Imp Note - the test should be performed using any of the following tests stated below.
Bile Escalin test, Oxidaze test, Blood Agar , Catalese Test, Mannitol salt agar .
Thank you !
You must find up your OWN dichotomous key for all the possible unknown organisms listed below. The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures .
Possible Organisms
Alcaligenes faecalis
Enterobacter aerogenes
Enterococcus faecalis
Escherichia coli
Proteus vulgaris
Pseudomonas aeruginosa
Salmonella arizoniae
Staphylococcus aureus
Staphylococcus epidermidis
Staphylococcus saprophyticus
Streptococcusbovis
Streptococcus pyogenes
Note : The test must be performed using any of this test Mannitol Salt Agar, DNAse test, Catalase test, coagulase test, Novobiocin Test, BILE ESCULIN AGAR (BEA) TEST, Oxidase test, Bacitracin Sensitivity, Blood Agar . Macconkey, Nitrate Reduction test.
Thank you so much in Advance !
Chapter 4 Solutions
Microbiology With Diseases By Taxonomy (6th Edition)
Ch. 4 - Prob. 1TMWCh. 4 - Prob. 2TMWCh. 4 - Prob. 3TMWCh. 4 - Prob. 4TMWCh. 4 - Prob. 1EDCSCh. 4 - Prob. 1MCCh. 4 - A nanometer is ________ than a micrometer. a. 10...Ch. 4 - Prob. 3MCCh. 4 - Curved glass lenses _________ light. a. refract b....Ch. 4 - Prob. 5MC
Ch. 4 - Prob. 6MCCh. 4 - Which of the following types of microscopes...Ch. 4 - Prob. 8MCCh. 4 - Prob. 9MCCh. 4 - Prob. 10MCCh. 4 - If an objective magnifies 40 and each binocular...Ch. 4 - The type of fixation developed by Koch for...Ch. 4 - Immersion oil ________ (increases/decreases) the...Ch. 4 - Prob. 4FIBCh. 4 - Prob. 5FIBCh. 4 - Prob. 1VICh. 4 - Label the microscope.Ch. 4 - Prob. 1SACh. 4 - Critique the following definition of magnification...Ch. 4 - Prob. 3SACh. 4 - Prob. 4SACh. 4 - Prob. 5SACh. 4 - Give three characteristics of a specific epithet.Ch. 4 - Prob. 7SACh. 4 - Prob. 8SACh. 4 - Prob. 1CTCh. 4 - Prob. 2CTCh. 4 - Prob. 3CTCh. 4 - Prob. 4CTCh. 4 - Prob. 5CTCh. 4 - In what ways are the Gram stain and the acid-fast...Ch. 4 - Microbiologists have announced the discovery of...Ch. 4 - Why is the genus name Coccus placed within...Ch. 4 - A clinician obtains a specimen of urine from a...Ch. 4 - Using the following terms, fill in the following...
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- Match the expected result (purple, red, or colorless) to the following descriptions of Gram-stained cells.Consult your chart at the beginning of this lab report if you need help remembering the correct Gramreaction for each species. Choices may be used more than once. Staphylococcus aureus before the primary stainarrow_forwardizzes/00071/take/questions/600381 Regarding Gram staining, what will be the appearance of the Gram-positive bacteria if... All steps are done correctly? The slide is not heat-fixed prior to staining? Crystal violet was omitted? lodine was omitted? Only iodine was used? Acetone:alcohol was omitted? Too much acetone:alcohol was used or was left on for too long? I Not enough acetone:alcohol was used? Carbol fuschin or safranin was omitted? W [Choose ] [Choose ] Purple Clear Pink Orange [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] 88 22°Carrow_forwardYou must write up your OWN dichotomous key for all the possible unknown organisms listed below. The first step of the key will be the Gram Stain and the second will be Gram negative. Subsequent steps will include biochemical tests only. Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures . Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes. Can you please help me find the dichotomous key . Thank you !arrow_forward
- Gram negative bacteria do not retain the stain when washed with alcohol.Comment.arrow_forwardCreate a dichotomous key Use the following bacteria and place them at the top of your key: Escherichia coli Klebsiella pneumoniae Staphylococcus epidermidis Bacillus subtilis Use the following five criteria to create the couplets: Morphology Gram reaction Acid Fast Reaction Endospore Positive/Negative Capsule Positive/Negativearrow_forwardOut of twelve tests that can be performed: Gram Stain Wet Mount Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase Test. Which four tests are the best fit to help identify bacteria that is unknown? Possible bacterias that are being observed are: Enterobacter aerogenes Escherichia coli Micrococcus luteus Proteus vulgaris Pseudomonas aeruginosa Salmonella typhimurium Serratia marcescens Staphylococcus aureus Staphylococcus epidermidisarrow_forward
- Complete the following table with your observations from the prepared slide Name of the Bacteria Type of Endospore Staining Technique (+ or −) Drawing Clostridium botulinum Clostridium perfringens Bacillus anthracisarrow_forwardplease label this in the correct order for gram staining. crystal violet, iodine, alcohol, and safraninarrow_forwardWrite the process/steps in Preparation of Bacillus atrophaeus culture using Blood agar plates and MacConkey agar plates. Note: This will perform the students so please write it in the easiest manner. Thanksarrow_forward
- S aureus Styphimurium S aureus S typhimurium Nutrient Agar Plates MacConkey Agar Plates S epidermidis Unknown S epidermidis Unknown S aureus S typhimurium S aureus S typhimurium Mannitol Salt Agar Plates Gram Stain S epidermidis Unknown S epidermidis Unknownarrow_forwardMatch the expected result (purple, red, or colorless) to the following descriptions of Gram-stained cells.Consult your chart at the beginning of this lab report if you need help remembering the correct Gramreaction for each species. Choices may be used more than once. Staphylococcus aureus after decolorizationarrow_forwardPut the following Steps of gram smear in order Create a bacteria Add crystal violet to the bacterial smear. Rinse with water after 60 seconds. Allow the smear to air dry and then heat fix it. Add gram iodine to the smear, let it sit for 60 seconds and rinse water decolorize with 95% ethanolfoe 5-10 seconds and then rise with water Blot dry with bibulous paper Add safranin to the smear and let sit for 60 seconds, then rinse with water Observe using oil immersionarrow_forward
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