Microbiology With Diseases By Taxonomy (6th Edition)
6th Edition
ISBN: 9780134832302
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 4, Problem 6MC
Summary Introduction
Introduction:
In transmission electron microscope (TEM), an image is produced by the transmission of electrons. A dehydrated and thinly sliced specimen is used for this. Light microscope is made up of multiple lenses through which the light reaches the specimen.
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FRET microscopy can be used for:
a. none of the above
b. electron microscopy
c. centrifugation
d.sensing kinase activity
e. all of the above
a. Do you open both eyes or close one eye when viewing a specimen under a microscope? Why? Why not?
b. In using a compound microscope, do you start with the low power objective (LPO) or the high power objective (HPO)? Why or Why not?
c. When using 60X, do you it together with the coarse adjustment knob or fine adjustment knob?
d. Why must you slowly turn the fine adjustment knob?
e. Is natural light or is artificial light used when using the microscope?
As with standard phase contrast microscopy,differential-interference contrast microscopy depends on differences in the ___________________ of a speciment to create high-contrast images.
a. refractive index
b. light absorption
c. natural fluorescence
d. walls and coverings
e. natural pigmentation
Chapter 4 Solutions
Microbiology With Diseases By Taxonomy (6th Edition)
Ch. 4 - Prob. 1TMWCh. 4 - Prob. 2TMWCh. 4 - Prob. 3TMWCh. 4 - Prob. 4TMWCh. 4 - Prob. 1EDCSCh. 4 - Prob. 1MCCh. 4 - A nanometer is ________ than a micrometer. a. 10...Ch. 4 - Prob. 3MCCh. 4 - Curved glass lenses _________ light. a. refract b....Ch. 4 - Prob. 5MC
Ch. 4 - Prob. 6MCCh. 4 - Which of the following types of microscopes...Ch. 4 - Prob. 8MCCh. 4 - Prob. 9MCCh. 4 - Prob. 10MCCh. 4 - If an objective magnifies 40 and each binocular...Ch. 4 - The type of fixation developed by Koch for...Ch. 4 - Immersion oil ________ (increases/decreases) the...Ch. 4 - Prob. 4FIBCh. 4 - Prob. 5FIBCh. 4 - Prob. 1VICh. 4 - Label the microscope.Ch. 4 - Prob. 1SACh. 4 - Critique the following definition of magnification...Ch. 4 - Prob. 3SACh. 4 - Prob. 4SACh. 4 - Prob. 5SACh. 4 - Give three characteristics of a specific epithet.Ch. 4 - Prob. 7SACh. 4 - Prob. 8SACh. 4 - Prob. 1CTCh. 4 - Prob. 2CTCh. 4 - Prob. 3CTCh. 4 - Prob. 4CTCh. 4 - Prob. 5CTCh. 4 - In what ways are the Gram stain and the acid-fast...Ch. 4 - Microbiologists have announced the discovery of...Ch. 4 - Why is the genus name Coccus placed within...Ch. 4 - A clinician obtains a specimen of urine from a...Ch. 4 - Using the following terms, fill in the following...
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- B. Write the function/s of each part of the microscope listed below. a. Eyepiece b. Draw tube c. Hemispheric prism housing d. Dust shield e. Revolving Nose Piece f. Objectives: Scanner LPO HPO ΟΙΟ g. Arm h. Coarse Adjustment knob i. Fine adjustment knob j. Slide holder & clip k. Rear knob I. Front knob m. Central aperture n. Condenser o. Iris diaphragm p. Mechanical Stage q. Mirror r. Mirror rack s. Stage adjustment knob t. Basearrow_forwardWhich microscopy technique is BEST at determining the presence of physical microbial interaction(s)? a Light microscopy. b Scanning or transmission electron microscopy. c Fluorescent microscopy. d Phase contrast microscopy.arrow_forwarda. How was the specimen prepared for the microscopy technique applied? (for e.g. stained with H&E stain, Gram stain, unstained) b. What is the microscopy technique and magnification used to obtain this image? c. What is the basic principle of image formation using this microscopy technique? d. What can be observed and concluded from the image of the specimen? e. Are there any potential aberrations present in this specimen image? Describe these and how they may affect interpretation of the result.arrow_forward
- A phase-contrast microscope differs from a brightfield microscope by having a a. blue filter in the ocular lens. b. diaphragm with an annular stop. c. phase plate in the objective lens. d. Both (b) and (c) are correct. e. All are correct.arrow_forwardThe resolving power of the microscope reveals: a. Whether or not oil is needed to see a microorganism. b. The maximum magnification possible with a particular objective. closely spaced objects to be seen clearly. c. Two closely spaced objects to be seen clearly. d. The magnification capacity of the microscope E.the amount of clearance between slide and objective lens.arrow_forwardIn light microscopy, when magnification is increased, the lens focuses closer to the sample. This makes the user more likely to ram the sample into the lens. Why is it common for the lens to focus closer to the sample at higher magnifications? What can be done to mitigate this risk?arrow_forward
- What is/are the characteristics of an ideal microscope? I. Low f-number. II. High f-number. III. Short wavelength of "light" used IV. Long wavelength of "light" used A. I only. B. II only. C. III only. D. Both I and III. E. Both II and IV.arrow_forwardA microscope that maintains focus when the objective magnification is increased is calleda. binocular.b. myopic.c. parfocal.d. refractive.e. resolute.arrow_forwardWhat part of the microscope adjusts the magnification of the microscope? Group of answer choices A. objective lens B. on/off switch C. iris D. course adjustmentarrow_forward
- What part of the microscope adjusts the amount of light projected onto the slide? Group of answer choices A. rheostat B. on/off switch C. iris D. course adjustmentarrow_forwardThe resolving power of a microscope is a function of a. the magnifying power of the lenses. b. the numerical aperture of the lenses. c. the wavelength of light. d. Both (a) and (b) are correct. e. Both (b) and (c) are correctarrow_forwardParagraph 3. If the ocular lens on a microscope has a magnification of 20OX, what is the total magnification of: a. a scanning lens with magnification of 4X b. a low-power lens with a magnification of 1oX c. a high power lens with a magnification of 40X d. an oil-immersion lens with a magnification of 100X 4. Choose how the letter "R" will appear as viewed under a microscope. R B R As viewed with the naked eye b. a. P. 5. True/False a. Begin the focusing process with the high-power objective. b. Store the microscope with the oil-immersion objective in position. c. Use tissue d. The coarse adjustment knob can be used with any objective lens. раper and water to clean the ocular and objective lenses.arrow_forward
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