Put the following Steps of gram smear in order Create a bacteria Add crystal violet to the bacterial smear. Rinse with water after 60 seconds. Allow the smear to air dry and then heat fix it.  Add gram iodine to the smear, let it sit for 60 seconds and rinse water decolorize with 95% ethanolfoe 5-10 seconds and then rise with water Blot dry with bibulous paper Add safranin to the smear and let sit for 60 seconds, then rinse with water Observe using oil immersion

during inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri dish is slightly open. Explain the purpose of these steps briefly.

Microorganism Type of Microorganism Gram Stain Shape Tryptic Soy Agar McConkey Agar Blood Agar EMB Agar Mannitol Salt Agar Bacillus cereus                   Bacillus megaterium                 Bacillus subtilis                   Candida albicans                 Escherichia coli                   Micrococcus luteus                 Neisseria sicca                   Proteus vulgaris                 Pseudomonas aeruginosa                 Serratia marcescens                 Staphylococcus aureus                 Staphylococcus epidermidis

A microbiologist sets up a serial dilution to analyze bacteria in raw milk.   Tube 1 – 0.1 mL milk diluted in 9.9 mL sterile water Tube 2 – 0.1 mL milk from Tube 1 diluted in 9.9 mL sterile water Tube 3 – 1.0 mL milk from Tube 2 diluted in 9.0 mL sterile water   What is the dilution of raw milk in Tube 3?

Out of all these tests, which 2 would help further understand this unknown lab. (Gram Stain and Growth Result is already done) Possible Tests (pick 2 that would help know the unknown bacteria) Gram stain morphology & arrangement color and growth characteristics on agar 37C, 30C and 25C (room temp) CAMP test starch hydrolysis test bacitracin susceptibility test glucose, lactose, sucrose, maltose fermentation tests methyl red/MR test Mannitol MacConkey Phenol Red Vogues-Proskauer/VP test citrate utilization test urea hydrolysis test phenylalanine deaminase test SIM test (Sulfide/Indole/Motility) Nitrate reduction test Oxidase test Catalase test

List the steps of the gram staining procedure in proper sequence and indicate the color of gram-positive and gram-negative bacteria after each step.

Dilute cells from an old culture 1:50 into 200 mL cultures. What volume of the old culture would you add to the new media?