Put the following Steps of gram smear in order Create a bacteria Add crystal violet to the bacterial smear. Rinse with water after 60 seconds. Allow the smear to air dry and then heat fix it.  Add gram iodine to the smear, let it sit for 60 seconds and rinse water decolorize with 95% ethanolfoe 5-10 seconds and then rise with water Blot dry with bibulous paper Add safranin to the smear and let sit for 60 seconds, then rinse with water Observe using oil immersion

Q: layer

A: The crystal violet eventually escapes from cytoplasm of the cell, if left too long for…

Q: what color would you expect for a gram positive microorganism for each incorrect mistakes while…

A: "Gram staining" is a technique used in microbiology. It is a method in which a bacterium can be…

Q: The acid fast bacterium, Mycobacterium smegmatis, is relatively safe for students to work with…

A: Mycobacteria are immobile, slow-growing (doubling time = 24 h), rod-shaped, gram-positive bacteria…

Q: ext/map/ aining Lab Report Place the descriptions into the correct boxes to test your understanding…

A: Gram staining is a method frequently used to distinguish between two sizable groups of bacteria…

Q: If you have a mixed (Gram positive and Gram negative) sample, and you complete a Gram stain, and all…

A: On the basis of lipid content in cell wall of bacteria these are classified into two types : Gram…

Q: Why do you have to boil the agar solution before dispensing it into tubes?

A: Agar is the medium which can used to grow the micro organisms in petri plates. The selected…

Q: Why bleach disinfectant will the most effective against probiotic?

A: Probiotics are live microorganisms that have health benefits when consumed. These are usually…

Q: Give two reasons for heating the slide after the smear is air dried?

A: Smear is the thin film of the sample that is spread over the slide to be observed in the microscope.…

Q: An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and…

A: Introduction: Acid-fast staining is a type of differential staining technique that was first…

Q: Move the images of bacterial cells into the chart to illustrate the color of gram-positive and…

A: Gram staining is a technique used to differentiate between two groups of bacteria namely…

Q: In your own words, explain how gram staining is done and how it works.

A: Gram staining is a popular method for distinguishing between two large groups of bacteria based on…

Q: If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is…

A: Option b

Q: 45-year-old male had been working in his garden when he scratched the knuckle of his right arm…

A: There are many types of/species of bacteria. Through different techniques and lab procedures,…

Q: Select All That Apply: Streptococcus can be found in this staining. O Diplococcus can be found in…

A: Bacteria are small unicellular organisms. They are found all over the world . Some of them can…

Q: Question 6 Describe what the Gram Stain shows: a. The organism is Gram Negative b. The organism is…

A: Introduction :- Bacteria are classified into two categories on the basis of the gram staining…

Q: You have a culture of bacteria and you performed an Acid Fast staining. You see red rod-shaped…

A: Introduction -  Acid-fast staining is a differential stainingused to differentiate acid fast and non…

Q: The below  photograph shows a Gram-stained slide viewed using a light microscope set at brightfield…

A: Gram's staining is a technique used to distinguish between Gram negative and Gram positive bacteria.…

Q: Which stain is used 1st after you heat fix the fly during the gram staining procedure

A: Gram staining is the procedure by which the gram positive and gram negative bacteria can be…

Q: Supposed you are to observe an unknown bacterial slide. In your initial observation, it appears to…

A: Microorganisms are defined as organisms that cannot be viewed by unaided eyes and require a…

Q: The bacterial cells in the image below were stained with a high concentration of carbolfuschin for…

A: Carbol fuchsin, carbolfuchsin, or carbol fuchsin is basically a mixture of "phenol" and basic…

Q: Gram E. coli 3nnell rod shaped 1000 x - around 1-2 micrometers long gative grom should be pink ich…

A: Gram Staining It is a differential bacterial staining techniques used to differentiate Bacteria…

Q: PREPARATION OF SMEARS: 1. Wash the slide thoroughly with a detergent, rinse with water and dry with…

A: PREPARATION OF SMEAR ( FLOWCHART)

Q: With what kind of gram stain do you use heat fixation? What is the purpose of heat fixation? What…

A: Gram stain Gram staining is a method of staining which is used to classify the bacterial species…

Q: BACTERIA THAT MOSTLY GROW ON MACCONKEY WELL APPEAR OF GRAM STAINING: AT THE END

A: On the basis of differential staining with a crystal violet-iodine complex and a safranin…

Q: two different

A: Gram staining- A test that checks for bacteria at the site of a suspected infection such as the…

Q: Bacterial smears are fixed before staining to make their walls permeable. accept stain, O affix the…

A: By staining microbial smears on a microscope slide, bacteria and bacterial components such as…

Q: A bacterial sample collected from a patient is processed using a Gram's stain. After staining, the…

A: Gram stain is a test that looks for bacteria in areas where an infection is suspected, such as the…

Q: Which step of the gram stain procedure is most critical?why?

A: Gram staining is one of the most common laboratories test to identify the different bacterial…

Q: Both crystal violet and saffron are basic Steve's and maybe used to do simple stains on…

A: Gram staining is the most important staining procedure used to distinguish the bacterial species…

Q: Which of the following is not meant to reduce contamination of yoru sample? avoiding hand contact…

A: A microbial culture is done to multiply the specific strains of the microorganisms under specific…

Q: What is the purpose of iodine in the Gram stain?   To act as a counterstain   None of the other…

A: There are various staining techniques that are used to stain different cellular structures. The Gram…

Q: Materials: .Bacterial cultures: E. coli & Staphylococcus aureus • Crystal violet (primary stain)…

A: Introduction:- Staining is the biochemical technique for coloring the specimens. There are different…

Q: Out of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and…

A: Bacteria are small, microscopic and unicellular organisms that can live in variety of environment.…

Q: Explain why some cells are pink and others are purple in Gram-stained bacterial smear.

A: Gram staining is a common method that is used to distinguish two large bacterial groups based on…

Q: In the Gram stain, gram-negative bacteria appear red for all the foll

A: Gram-negative bacteria appear red because they do not retain the crystal violet stain used in the…

Q: What is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide…

A: The staining of microorganisms is very much important that helps us in identifying the morphology…

Q: You collected a sample from a patient with a suspected STI. After Gram staining, you observed this…

A: The staining of bacteria is an important method of classifying the bacteria There could be multiple…

Q: please label this in the correct order for gram staining.  crystal violet, iodine, alcohol, and…

A: Gram staining is a method of staining useful for identifying and classifying bacteria into two major…

Q: Why is it essential that smears be air-dried?  Why can’t they be gently heated over a flame to speed…

A: Introduction :  A bacterial smear is a tiny amount of culture that has been applied to the slide's…

Q: escribe the microscopic morphology of each sample observed with a Gram stain (S. aureus, P.…

A: Gram stain is done to diffrentiate between Gram positive and Gram negative bacterias.  Hans…

Q: What is the purpose of a bacterial smear? What is the purpose of a bacterial smear? It spreads the…

A: Introduction:  The bacterial smear technique is a routine procedure performed to prepare the…

Q: which of the following can be used as a disinfectant on a table top and works by denaturing bacteria…

A: Introduction :  Disinfection is the process of eliminating or decreasing bacteria to a level where…

Q: Contrast the overall cell wall compositions of Gram positive and Gram negative bacteria, and go…

A: The bacteria are classified according to their cell wall composition into Gram positive and Gram…

Q: Describe the bacteria observed in different staining procedures. Please refer to the photo - Direct…

A: The cell wall of most bacteria has an overall net negative charge and thus can be stained directly…