Put the following Steps of gram smear in order Create a bacteria Add crystal violet to the bacterial smear. Rinse with water after 60 seconds. Allow the smear to air dry and then heat fix it.  Add gram iodine to the smear, let it sit for 60 seconds and rinse water decolorize with 95% ethanolfoe 5-10 seconds and then rise with water Blot dry with bibulous paper Add safranin to the smear and let sit for 60 seconds, then rinse with water Observe using oil immersion

Q: What is the purpose of fixing a smear?

A: Smear- a smear is a thin layer of a sample such as bacterial cells or blood spread evenly on a glass…

Q: Peptidoglycan on bacterial cell walls is stained by crystal violet dye and for this reason those…

A: True

Q: What is the function of the mordant used in the Gram staining procedure? If the iodine step is…

A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…

Q: student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step…

A: 5. The loop was flamed

Q: Why is it important to limit the quantity of cells used to prepare a smear?

A: A thin layer of cells or tissues that are taken from the body of an organism under study which is…

Q: When preparing a "wet mount" slide of your own cheek cells, what is the name of the wet medium that…

A: Cheek cells are human cells and eukaryotic cells which can be visualized under the microscope. These…

Q: How can natural pigments produced by bacteria reveal their Gram stain reaction? O Natural pigments…

A: Gram staining is a procedure through which a bacterial species can be classified and distinguishes…

Q: the shape of the bacterial cells for each of the two yogurt bacteria. Be sure to use the terms for…

A: Both streptococcus thermophilus and lactobacillus acidophilus are probiotic Bacteria that are found…

Q: 1 ml from main culture 1ml 99999 5 ml LB 1 ml 5 ml LB 5ml LB 1ml 5 ml LB 1 ml 5 ml LB You took 250…

A: To calculate the number of cfu/ml bacteria in the sample, we need to use the dilution factor and the…

Q: ext/map/ aining Lab Report Place the descriptions into the correct boxes to test your understanding…

A: Gram staining is a method frequently used to distinguish between two sizable groups of bacteria…

Q: If you have a mixed (Gram positive and Gram negative) sample, and you complete a Gram stain, and all…

A: On the basis of lipid content in cell wall of bacteria these are classified into two types : Gram…

Q: When you interpret a Gram-stained smear, you should also describe the morphology (shape) of the…

A: Bacteria These are prokaryotic organisms which kack membrane bound organelles. Through the technique…

Q: Is this a microbial formation visible to the naked eye? Explain. Leg abscess aspirate swabbed onto…

A: Microbes are biological entities that can not be seen by the naked eyes due to their very small…

Q: A capsule stain is kind of: A Simple and Negative Stain B Differential and negative stain C…

A: Introduction  Capsule is the outer most covering, which is present even outside the cell wall. This…

Q: Which of the following are true about a differential stain. There may be more than one answer!…

A: A microscopic organism made of one cell is referred to as a prokaryote. Prokaryotic organisms do…

Q: Move the images of bacterial cells into the chart to illustrate the color of gram-positive and…

A: Gram staining is a technique used to differentiate between two groups of bacteria namely…

Q: Which of the following places the steps of the Gram stain in the correct order? 1-Alcohol-acetone…

A: There are two types of bacteria, i.e gram-positive and gram-negative.

Q: A Gram stain indicates purple cells that are round and attached in chains. Which of the following…

A: Gram staining is a simple technique, in which two categories of bacteria are identified based on…

Q: If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is…

A: Option b

Q: Which of the following serves as a counterstain for the gram-stain technique? It also happens to be…

A: Gram staining is a technique used to differentiate two groups of bacteria based on the different…

Q: Using a diagram similar to those provided, describe how serial dilutions and the Standard Plate…

A: The stepwise dilution of a solution is termed as a serial dilution. Generally, the dilution…

Q: You have a culture of bacteria and you performed an Acid Fast staining. You see red rod-shaped…

A: Introduction -  Acid-fast staining is a differential stainingused to differentiate acid fast and non…

Q: Which stain is used 1st after you heat fix the fly during the gram staining procedure

A: Gram staining is the procedure by which the gram positive and gram negative bacteria can be…

Q: a. After conducting the Gram staining procedures and you observed that all cells are stained with…

A: The microorganisms that can be found in several areas of the habitat and come under the category of…

Q: You are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the…

A: Gram negative bacteria: these bacteria are enclosed in hard capsule and don't retain the crystal…

Q: Can you please help me describe the colony apperance on these two stains? Thank you! Growth on Blood…

A: The chocolate agar and the blood agar media both have the almost same composition. The only…

Q: A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution…

A: A sample of your blood, urine, skin, or any part of your body will be taken during a bacteria…

Q: The unknown observations are shown as a mixed stain, gram positive, and gram negative. The next…

A: There are two types of bacteria present i.e., gram positive bacteria and the gram negative bacteria…

Q: colony-forming units

A: Colony Forming Unit:It is designated as CFU.It can defined as the unit of measurement used to…

Q: This is how a hanging-drop slide is prepared. Figure from Macedo, Wikimedia Commons, 2016. 2 3 4…

A: Observing and studying living microorganisms, such as bacteria or protozoa under a microscope can be…

Q: Which of the following is not meant to reduce contamination of yoru sample? avoiding hand contact…

A: A microbial culture is done to multiply the specific strains of the microorganisms under specific…

Q: Below are photos of 3 bacteria, letter lableled, inoculated onto MSA plates and incubated. Note no…

A: Microbiology is the study of microscopic organisms that are mostly not visible to the naked eye.…

Q: Out of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and…

A: Bacteria are small, microscopic and unicellular organisms that can live in variety of environment.…

Q: Explain why some cells are pink and others are purple in Gram-stained bacterial smear.

A: Gram staining is a common method that is used to distinguish two large bacterial groups based on…

Q: What is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide…

A: The staining of microorganisms is very much important that helps us in identifying the morphology…

Q: Explain the relationship between bacterial cell wall with gram-positive and gram-negative in the…

A: Gram staining is the standard laboratory practice for distinguishing between two broad species of…

Q: The figure attached shows the colonies on Eosin Methylene Blue Agar (EMBA) plate. Make a description…

A: METHYLENE BLUE AGAR (EMB Agar) Methylene Blue Eosin Agar is a differential medium used for the…

Q: please label this in the correct order for gram staining.  crystal violet, iodine, alcohol, and…

A: Gram staining is a method of staining useful for identifying and classifying bacteria into two major…

Q: Based on the photo, describe the bacteria observed from the different staining technique. -…

A: Microbiology was a blessing in the eradication of mass-destructive epidemics such as rabies,…

Q: Describe the color and appearance of a Gram positive and Gram negative bacteria as it is treated at…

A: A Gram stain test looks for bacteria in areas where an infection is thought to have occurred,…

Q: move the images of bacterial cells into the chart to illustrate the color of gram-positive and gram…

A: answer given below

Q: With liquid media such as broth, is it possible to determine if the culture is pure using the naked…

A: A culture is a method by which microbes are allowed to reproduce and multiply in predetermined…

Q: if there are features of the gram stain that are not as expected what possible sources or error may…

A: Gram staining is a general technique that is used to differentiate two larger groups of bacteria…

Q: am-positive (+) bacteria with a thin peptidoglycan layer traps the crystal violet stain. Select…

A: Bacteria are the single celled microscopic organisms. These are can not be seen through the nalked…

Q: Describe the bacteria observed in different staining procedures. Please refer to the photo - Direct…

A: The cell wall of most bacteria has an overall net negative charge and thus can be stained directly…