Put the following Steps of gram smear in order Create a bacteria Add crystal violet to the bacterial smear. Rinse with water after 60 seconds. Allow the smear to air dry and then heat fix it.  Add gram iodine to the smear, let it sit for 60 seconds and rinse water decolorize with 95% ethanolfoe 5-10 seconds and then rise with water Blot dry with bibulous paper Add safranin to the smear and let sit for 60 seconds, then rinse with water Observe using oil immersion

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