Principles of Instrumental Analysis
7th Edition
ISBN: 9781305577213
Author: Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher: Cengage Learning
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Chapter 34, Problem 34.14QAP
Interpretation Introduction
Interpretation:
When
Concept introduction:
Centrifugation is based on the partition of particles by sedimentation. The pace of division in a suspension of particles principally relies upon the molecule density and size.
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7. The sedimentation and diffusion coefficients for myoglobin in dilute aqueous solution at 20°C are
2.04 x 10-13 s and 1.13 x 10-10 m²s¹, respectively. The partial specific volume of the protein is 0.741
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A molecular exclusion column has a diameter of 7.8 mm and a length of 30 cm. The solid portion of the particles occupies 20% of the volume, the pores occupy 40%, and the volume between particles occupies 40%. (a) At what volume would totally excluded molecules be expected to emerge? (b) At what volume would the smallest molecules be expected? (c) A mixture of polymers of various molecular masses is eluted between 23 and 27 mL. What does this imply about the retention mechanism for these solutes on the column?
22-30. (a) The mean free path is the average distance a molecule
travels before colliding with another molecule. The mean free
path, A, is given by A = kT/(V20P), where k is Boltzmann’s
constant, T is temperature (K), P is pressure (Pa), and o is the
collision cross section. For a molecule with a diameter d, the col-
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molecule it encounters. The magnetic sector mass spectrometer is
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Chapter 34 Solutions
Principles of Instrumental Analysis
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