Principles of Instrumental Analysis
Principles of Instrumental Analysis
7th Edition
ISBN: 9781305577213
Author: Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher: Cengage Learning
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Chapter 34, Problem 34.10QAP
Interpretation Introduction

Interpretation:

The given enzyme has the translational diffusion coefficient of 3.75×107cm2s1 and temperature 20 degree Celsius.The hydrodynamic diameter of the particle is to be determined.

Concept introduction:

Strokes −Einstein relationship can estimate the time autocorrelation function of a mass of protein. The translational diffusion coefficient of a flexible polypeptide having single chain and is kept in a dilute solution can be calculated from Einstein theory.

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Suppose the concentration of a solute decays linearly along the length of a container according to c(x) = c0 - αc0x, where c0 is the concentration at x = 0. Calculate the thermodynamic force on the solute at 25 °C and at x = 10 cm and 20 cm given that the concentration falls to 1/2c0 when x = 10 cm. Hint: Start by finding the value of α.
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