(a) Interpretation: The description for the influence of temperature for a reversed-phase chromatographic separation of a steroid mixture is to be stated. Concept introduction: The chromatography is the method of separation of the mixtures of the substances in their compounds. There are various forms of chromatography and all works on the same principle. The mobile phase of the compounds flows through the stationary phase of the compounds and carries the mixture of components along with it. The higher performance liquid chromatography is used for the liquid-liquid chromatography and both the phases consists only liquid solutions.

Question

Differentiate normal phase chromatography from reverse phase chromatography. From which of the two governs the thin layer chromatography of plant pigments? How do you say so?

Answer 1

Question

Chapter 28, Problem 28.19QAP

Interpretation Introduction

(a)

Interpretation:

The description for the influence of temperature for a reversed-phase chromatographic separation of a steroid mixture is to be stated.

Concept introduction:

The chromatography is the method of separation of the mixtures of the substances in their compounds. There are various forms of chromatography and all works on the same principle. The mobile phase of the compounds flows through the stationary phase of the compounds and carries the mixture of components along with it.

The higher performance liquid chromatography is used for the liquid-liquid chromatography and both the phases consists only liquid solutions.

Interpretation Introduction

(b)

Interpretation:

The description for the influence of temperature for an adsorption chromatographic separation of a mixture of closely related isomers is to be stated.

Concept introduction:

The chromatography is the method of separation of the mixtures of the substances in their compounds. There are various forms of chromatography and all works on the same principle. The mobile phase of the compounds flows through the stationary phase of the compounds and carries the mixture of components along with it.

The gas chromatography is also known as gas-solid chromatography in which solid is present in the stationary phase and gas is present in the mobile phase.

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