(a)
Interpretation:
The order of the compounds containing the reversed-phase packing is to be stated.
Concept introduction:
The bonded phase has non polar nature in the reversed-phase packing. The non polar solute attracted towards the reverse phase packing and spent more time on the non polar bonded packing. Due to this the non-polar solute elute at the last. The polar solute will not attracted towards the bonded packing and also elute first from the column.
(b)
Interpretation:
The order of the compounds containing the reversed-phase packing is to be stated.
Concept introduction:
The bonded phase has non polar nature in the reversed-phase packing. The non polar solute attracted towards the reverse phase packing and spent more time on the non polar bonded packing. Due to this the non-polar solute elute at the last. The polar solute will not attracted towards the bonded packing and also elute first from the column.
Trending nowThis is a popular solution!
Chapter 28 Solutions
Principles of Instrumental Analysis
- Which of the following pairs of compounds would be most easily separated by thin layer chromatography: n-octyl alcohol and 1-octene, hexadecane and octadecane, or chlorobenzene and bromobenzene?arrow_forward(iii) What would be the effect on the retention time and order of eluting if the C18 column is substituted with a -CN column?arrow_forwardExplain why TLC cannot be used for some volatile low molecular weight substances such as toluene.arrow_forward
- You performed an isocratic reversed-phase HPLC separation of phenol, toluene and catechol using a DAD detector set at 275nm and obtained the chromatogram shown below. What are the polarities of the mobile phase and stationary phase in this separation? What order would you expect these compounds to elute in? Why? What changes could you make to the mobile phase to decrease the elution time of the last compound and improve its peak shape? If all three species are present in the analyzed solution at the same concentration, what might account for different in peak height for the first two compounds eluting from the column? What change to the instrument set-up could you make to increase the detector response of this analyte?arrow_forwardColumn chromatography is also very useful for separating mixtures of organic compounds that are not coloured. Briefly explain how you could use column chromatography for the separation of a colourless mixture, and how you could identify your separated compounds.arrow_forwardWhy is CCL4 preferred over acetone as a solvent in running IR spectra of solids?arrow_forward
- The compounds biphenyl and naphthalene are presented in a mixture. Can hexane be used as a solvent to separate the compounds by column chromatography with silica gel as the stationary phase? Why or why not.arrow_forward(b) Acetone shows two absorption bands at 2 max = 270 nm and 187 nm. Which of the two is a low intensity absorption band and why is it of low intensity?arrow_forwardA particular mixture was seperated into three components usin paper chromatography. the Rf value of dye A is 0.78, the Rf of dye B is 0.43 and the Rf of dye C is 0.19. What component of the mixture A,B,C is most attracted in the stationery phase and why?arrow_forward
- Q2) In the thin layer chromatography experiment, calculate the relative mobility for the two dyes if the distance traveled by the solvent (ethanol) was 3 cm, the distance traveled by the purple dye was 2.5 cm, and 1.5 cm for the red dye? Which dye is considered less soluble in ethanol?arrow_forwardAcrolein (CH2=CH-CHO) shows two bands , at 210 nm (11,500) and 315 nm (15) . Assign these bands.arrow_forwardHow would you discuss method of separation and identification of compounds in TLC?arrow_forward
- ChemistryChemistryISBN:9781305957404Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCostePublisher:Cengage LearningChemistryChemistryISBN:9781259911156Author:Raymond Chang Dr., Jason Overby ProfessorPublisher:McGraw-Hill EducationPrinciples of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage Learning
- Organic ChemistryChemistryISBN:9780078021558Author:Janice Gorzynski Smith Dr.Publisher:McGraw-Hill EducationChemistry: Principles and ReactionsChemistryISBN:9781305079373Author:William L. Masterton, Cecile N. HurleyPublisher:Cengage LearningElementary Principles of Chemical Processes, Bind...ChemistryISBN:9781118431221Author:Richard M. Felder, Ronald W. Rousseau, Lisa G. BullardPublisher:WILEY