Histidine at pH 6 .8 migrates to the cathode or anode on electrophoresis has to be given. Concept Introduction: On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

Question

The activity of an enzyme requires a glutamic acid to display its -COOH functional group in the protonated state. Suppose the pK, of the -COOH group is 4.07. (a) Will the enzyme be more active at pH 3.5 or 4.5? Explain. (b) What fraction of the enzymes will be active at pH = 4.07? Explain. (c) At what pH will the enzyme show 78% of maximal activity?

Answer 1

Question

Chapter 27, Problem 27.36P

(a)

Interpretation Introduction

Interpretation:

Histidine at pH 6.8 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(b)

Interpretation Introduction

Interpretation:

Lysine at pH 6.8 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(c)

Interpretation Introduction

Interpretation:

Glutamic acid at pH 4.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(d)

Interpretation Introduction

Interpretation:

Glutamine at pH 4.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(e)

Interpretation Introduction

Interpretation:

Glu-Ile-Val at pH 6.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(f)

Interpretation Introduction

Interpretation:

Lys-Gln-Tyr at pH 6.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

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The activity of an enzyme requires a glutamic acid to display its -COOH functional group in the protonated state. Suppose the pK, of the -COOH group is 4.07. (a) Will the enzyme be more active at pH 3.5 or 4.5? Explain. (b) What fraction of the enzymes will be active at pH = 4.07? Explain. (c) At what pH will the enzyme show 78% of maximal activity?

2. (a) Describe any two of the secondary structure elements found in peptides. (b) Why do Glycine and Proline destabilise an a-helix secondary structure element? (c) Provide a definition of the term isoelectric point? (d) Using the information given below, calculate the pl of the amino acid citrulline. H₂N pK₂ = 9.23 H₂N. NH COOH pK₂ = 2.27

Indicate whether each statement is true or false. (a) Tryptophan is an aromatic amino acid. (b) Lysine is positively charged at pH 7. (c) Asparagine has two amide bonds. (d) Isoleucine and leucine are enantiomers. (e) Valine is probably more water-soluble than arginine.

Answer 2

Question

Chapter 27, Problem 27.36P

(a)

Interpretation Introduction

Interpretation:

Histidine at pH 6.8 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(b)

Interpretation Introduction

Interpretation:

Lysine at pH 6.8 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(c)

Interpretation Introduction

Interpretation:

Glutamic acid at pH 4.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(d)

Interpretation Introduction

Interpretation:

Glutamine at pH 4.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(e)

Interpretation Introduction

Interpretation:

Glu-Ile-Val at pH 6.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(f)

Interpretation Introduction

Interpretation:

Lys-Gln-Tyr at pH 6.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

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Answer 3

Question

Chapter 27, Problem 27.36P

(a)

Interpretation Introduction

Interpretation:

Histidine at pH 6.8 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(b)

Interpretation Introduction

Interpretation:

Lysine at pH 6.8 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(c)

Interpretation Introduction

Interpretation:

Glutamic acid at pH 4.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(d)

Interpretation Introduction

Interpretation:

Glutamine at pH 4.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(e)

Interpretation Introduction

Interpretation:

Glu-Ile-Val at pH 6.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(f)

Interpretation Introduction

Interpretation:

Lys-Gln-Tyr at pH 6.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

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Answer 4

Question

Chapter 27, Problem 27.36P

(a)

Interpretation Introduction

Interpretation:

Histidine at pH 6.8 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(b)

Interpretation Introduction

Interpretation:

Lysine at pH 6.8 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(c)

Interpretation Introduction

Interpretation:

Glutamic acid at pH 4.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(d)

Interpretation Introduction

Interpretation:

Glutamine at pH 4.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(e)

Interpretation Introduction

Interpretation:

Glu-Ile-Val at pH 6.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(f)

Interpretation Introduction

Interpretation:

Lys-Gln-Tyr at pH 6.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

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Answer 5

Chapter 27, Problem 27.36P

(a)

Interpretation Introduction

Interpretation:

Histidine at pH 6.8 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(b)

Interpretation Introduction

Interpretation:

Lysine at pH 6.8 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(c)

Interpretation Introduction

Interpretation:

Glutamic acid at pH 4.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(d)

Interpretation Introduction

Interpretation:

Glutamine at pH 4.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(e)

Interpretation Introduction

Interpretation:

Glu-Ile-Val at pH 6.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

(f)

Interpretation Introduction

Interpretation:

Lys-Gln-Tyr at pH 6.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

Answer 6

Chapter 27, Problem 27.36P

(a)

Interpretation Introduction

Interpretation:

Histidine at pH 6.8 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

Answer 7

Chapter 27, Problem 27.36P

(a)

Interpretation Introduction

Interpretation:

Histidine at pH 6.8 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

Answer 8

(b)

Interpretation Introduction

Interpretation:

Lysine at pH 6.8 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

Answer 9

(b)

Interpretation Introduction

Interpretation:

Lysine at pH 6.8 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

Answer 10

(c)

Interpretation Introduction

Interpretation:

Glutamic acid at pH 4.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

Answer 11

(c)

Interpretation Introduction

Interpretation:

Glutamic acid at pH 4.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

Answer 12

(d)

Interpretation Introduction

Interpretation:

Glutamine at pH 4.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

Answer 13

(d)

Interpretation Introduction

Interpretation:

Glutamine at pH 4.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

Answer 14

(e)

Interpretation Introduction

Interpretation:

Glu-Ile-Val at pH 6.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

Answer 15

(e)

Interpretation Introduction

Interpretation:

Glu-Ile-Val at pH 6.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

Answer 16

(f)

Interpretation Introduction

Interpretation:

Lys-Gln-Tyr at pH 6.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

Answer 17

(f)

Interpretation Introduction

Interpretation:

Lys-Gln-Tyr at pH 6.0 migrates to the cathode or anode on electrophoresis has to be given.

Concept Introduction:

On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.

Answer 18

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Ch. 27.1 - Of the 20 protein-derived amino acids shown in...Ch. 27.2 - Prob. 27.2P Ch. 27.2 - Prob. 27.3P Ch. 27.3 - Draw a structural formula for Lys-Phe-Ala. Label...Ch. 27.4 - Which of these tripeptides are hydrolyzed by...Ch. 27.4 - Deduce the amino acid sequence of an undecapeptide...Ch. 27.6 - Prob. 27.7P Ch. 27 - What amino acid does each abbreviation stand for?...Ch. 27 - The configuration of the chiral center in -amino...Ch. 27 - Assign an R or S configuration to the chiral...

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