Concept explainers
Data analysis If the excision of an F plasmid Is not precise, and some f coli DNA is added to the F plasmid, what are the consequences when this plasmid is transferred to a new cell?
To determine: The consequences of plasmid transference if the plasmid excision is not precise.
Introduction: The exchange of F plasmid takes place from the F+, which is a donor, to the F- that acts as the recipient. The F factor is transferred by the process called conjugation. The F plasmid has genes that are required for conjugation as well as infer some additional properties to the bacteria.
Explanation of Solution
The plasmid and the fragments of the genetic material can be excised with the help of specific enzymes known as restriction nucleases. These can be endonuclease or exonuclease. The plasmids need to be excised precisely so that the sticky ends or the complementary sequence ends can bind with the genetic material of the host organisms.
If the plasmid is not cut precisely then the frequency of the plasmids to combine with the genetic material of the bacteria (E.coli) will be reduced significantly. The few plasmids that have joined themselves to the bacterial genome will be very difficult to identify as the boundaries of the plasmid and the host genome will not be accurately known. To prevent such errors the endonuclease that makes precise cuts are preferred for cutting the plasmid and the host’s DNA.
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Chapter 27 Solutions
<LCPO> BIOLOGY
- Plasmid alone should be ....... after adding the enzyme dna ligase. become circular single stranded degraded remain lineararrow_forwardpXO1 and pXO2 are large plasmids – large pieces of DNA that are not the chromosome that can replicate independently of the chromosome. What can a Bacillus anthracis cell with a pXO2 plasmid do that a cell without pXO2 can’t? I need help answering this quwhstion as short as possible based of what is said in the paperarrow_forwardSmall extrachromosomal rings of DNA, often contain genes that give bacterial cells genetic advantages: * none is correct O chromatin Onucleoplasm nucleoid plasmidsarrow_forward
- Plate LB-Plasmid LB + Plasmid LB/amp - Plasmid Yes Yes No + LB LB/amp + Plasmid Yes LB Prediction Reeson Plate only contains Yes food/bacteria Plate only contains Yes food/bacteria Bacteria will be killed by ampicillin Bacteria has ampicillin resistance LB Click on pic to zoom Click on pic to zoom -LB Observed Result No Yes chlamp x Click on pic to zoom + LB/AMP Blamp Click on pic to zoom - LB/AMParrow_forwardVirology 8. A positive sense RNA sequence is given below. Give the correct sequence of the CDNA first strand synthesised by reverse transcriptase. 5'-UUCCGAUAUCGGCACUGAGUA-3' 5'- -3'arrow_forwardhelp me with this multiple choice question please, this is molecular and cell biology Visualization of an electrophoresed agarose gel (D) Plasmid DNA cannot be visualized on an agarose gel (A) Plasmid DNA may be visualized as multiple supercoiled forms (B) Visualized plasmid DNA is always linear Ⓒ(C) Visualized plasmid DNA is always circulararrow_forward
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