Inquiry Into Life (16th Edition)
16th Edition
ISBN: 9781260231700
Author: Sylvia S. Mader, Michael Windelspecht
Publisher: McGraw Hill Education
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Textbook Question
Chapter 26, Problem 6A
In this process, cells are removed from the body and Victoria is used to insert a gene of interest.
- DNA fingerprint
- In vivo gene therapy
- Ex vivo gene therapy
- DNA sequencing
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Explain how and why PCR can be used to amplify DNA. Describe the steps in the process. Be sure to address the role of Taq DNA polymerase.
RECOMBINANT DNA
BRIEFLY, DESCRIBE RECOMBINANT DNA AND GIVE ONE CONCRETE EXAMPLE. EVALUATE THE SIGNIFICANCE/PRACTICAL APPLICATIONS OF THIS DNA TECHNOLOGY BY CONSIDERING ETHICAL AND MORAL IMPLICATIONS BEHIND IT.
RECOMBINANT DNA
EXAMPLE:
MODIFIED TRAIT
GENE MODIFICATION
RECIPIENT ORGANISM
FIELDS OF APPLICATION
ALSO, WHAT ARE YOUR INSIGHTS? IN 2 TO 3 SENTENCES.
Why is using genomic DNA in gene therapy more efficacious?
Chapter 26 Solutions
Inquiry Into Life (16th Edition)
Ch. 26.1 - Describe the steps in forming Recombinant DNA.Ch. 26.1 - Discuss how the polymerase chain reaction works.Ch. 26.1 - Prob. 3LOCh. 26.1 - Prob. 1QTCCh. 26.1 - Prob. 2QTCCh. 26.1 - Prob. 1CYPCh. 26.1 - Explain how the PCR reaction amplifies a segment...Ch. 26.1 - Prob. 3CYPCh. 26.1 - Prob. 1AQTCCh. 26.1 - Prob. 2AQTC
Ch. 26.2 - Prob. 1LOCh. 26.2 - Prob. 2LOCh. 26.2 - Prob. 1QTCCh. 26.2 - Prob. 2QTCCh. 26.2 - Prob. 3QTCCh. 26.2 - Prob. 1CYPCh. 26.2 - Prob. 2CYPCh. 26.3 - Compare and contrast in vivo and ex vivo gene...Ch. 26.3 - Prob. 2LOCh. 26.3 - Prob. 1CYPCh. 26.3 - Prob. 2CYPCh. 26.3 - Prob. 3CYPCh. 26.4 - Prob. 1LOCh. 26.4 - Prob. 2LOCh. 26.4 - Prob. 3LOCh. 26.4 - Prob. 1CYPCh. 26.4 - Explain how comparative genomics can provide...Ch. 26.4 - Prob. 3CYPCh. 26 - Prob. S25.1BYBCh. 26 - Prob. S25.2BYBCh. 26 - Prob. S25.3BYBCh. 26 - Prob. 1ACh. 26 - Prob. 2ACh. 26 - The polymerase chain reaction Use RNA polymerase...Ch. 26 - Prob. 4ACh. 26 - Prob. 5ACh. 26 - In this process, cells are removed from the body...Ch. 26 - When a cloned gene is used to modify a human...Ch. 26 - Prob. 8ACh. 26 - Prob. 9ACh. 26 - Prob. 10ACh. 26 - Prob. 1TCCh. 26 - In a genomic comparison between humans and yeast,...
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- Which of the following protocols is BEST used for introducing exogenous DNA into a cell with a thick outer barrier (e.g. plant cell wall)? electroporation O heat-shock transformation gene gun Olipofectionarrow_forwardBriefly present experimental and practical benefits of using PCR in DNA cloning process. Give some examples of clinical applications.arrow_forwardRestriction enzymes and DNA ligase are key components when preparing cloning vectors. True or False.arrow_forward
- Which is a complete list of the ingredients that are essential for PCR? nucleotides, DNA template, Taq polymerase, and plasmids nucleotides, DNA template, DNA ligase, and plasmids nucleotides, DNA template, Taq polymerase, and primers restriction enzymes, DNA template, Taq polymerase, and primers nucleotides, DNA template, DNA ligase, and primersarrow_forwardDirect modification of genes may be achieved using what? (Choose one below): DNA microarray CRISPR-Cas system Hybridizationarrow_forwardRegarding STR markers used in forensic science. Tick all the correct statements: no correct statement the PCR primers used to amplify STRs are located in the repeat units PCR primers to amplify STRs are located on both sides of the repeat units the PCR primers used to amplify STRs are coupled to a fluorochrome which is essential for the detection of amplicons they are absent from the gonosomes the allelic frequencies of STR markers vary according to the ethnicity of the individuals genotyped they are present homogeneously throughout the nuclear genomearrow_forward
- Gel Electrophoresis is used in many different forms to learn about DNA, RNA or proteins. Research one laboratory method or technique that uses DNA electrophoresis in order to learn more or make determinations about DNA, RNA or Proteins. Name of technique or method Brief description of what the electrophoresis results are used for in the method. Include a link to your resources Include an image of the results or technique you describearrow_forwardThe enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy. Explain what they are used for. Also mention the actual biological function of the respective enzymes. T7 RNA polymerase reverse transcriptase RNaseHarrow_forwardBriefly outline the steps of DNA extraction as carried out in a lab for the purposes of sequencingarrow_forward
- Please place the stages of a CRISPR-cas9 gene editing workflow in the correct order below. Note, there is one incorrect and state why? Sequence Select for correctly gene edited cells (using antibiotic resistance and/or colour production for example) Clonal isolation Synthesise DNA insert oligonucleotides Clonal characterisation (analysis of phenotype) Add green fluorescent protein gene sequence into plasmid to aid selection of correctly transfected cells. Transfect cells Clone into CRISPR-cas9 expression vector Purify plasmids Design a set of targeting sequences using algorithms.arrow_forwardCompare and contrast ex vivo and in vivo gene therapy as approaches for delivering therapeutic genes.arrow_forwardCloned human embryonic stem cells would be useful for which of the following reasons: Group of answer choices They would be a useful step towards the goal of creating cloned human babies They would allow the creation of patient-specific, immunologically-matched ES lines so that patient's immune systems would not attack the stem cells they were treated with Following treatment with a restriction enzyme, they could be inserted into a useful vectorarrow_forward
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