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- Ehat primer sets could be amplify the following DNA sequence? AATACGTCGCATGGggatccttttttatgcatgMicroarray hybridization is used mostly in transcript profiling or assaying DNA variation. Although the technology for establishing DNA microarrays was developed only recently, numerous applications have already been developed and their impact on future biomedical research and diagnostic approaches is expected to be profound. Give some examples of the practical use of this technique.The best molecular technique to quantify the gene transcripts is (write in full).
- In selecting target cells to receive a transferred gene in gene therapy, what factors do you think would have to be taken into account?Hi, I would like to know which program is used for the graphical presentation of the results of a meta-analysis of genome-wide linkage scans?Briefly explain how synthetic probes are created to screen a DNA library when the protein encoded by the gene is known.
- RNA sequencing can detect DNA mutations typically easier to analyze can detect transcripts with very low abundance Both Answer Bank used for gene-expression analysis Microarray requires transcript-specific probesThe enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy. Explain what they are used for. Also mention the actual biological function of the respective enzymes. 1) RNaseHEcoRI recognizes G A-A-T-T-C sequence and cleave/ cut between G and A. How will the DNA fragments look like if EcoRI is used for the DNA below? How many fragments are produced? 5- AAAGATTTGAATTTCGAATTCAATTTAAGAATTCCCTTAGAATTTCC -¹3
- In biotechnology, gene cloning is a very important technique. A vector is normally required to perform this process. The vector commonly used to transform a bacterial host cell is the plasmid. State the three (3) important regions of the plasmid. Elaborate your answer.Why is genome editing by CRISPR-Cas advantageous over traditionalmethods for creating knockout or transgenic animals?Explain your answers.Briefly explain why RNA-seq gives more information about the transcriptome than does microarray analysis.