Inquiry Into Life (16th Edition)
16th Edition
ISBN: 9781260231700
Author: Sylvia S. Mader, Michael Windelspecht
Publisher: McGraw Hill Education
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Chapter 26, Problem 3A
The polymerase chain reaction
- Use RNA polymerase
- takes place in huge bioreactors.
- uses a temperature- insensitive enzyme
- Makes lots of nonidentical copies of DNA
- All of these are correct
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Compare and contrast the properties of DNA polymerase and RNA polymerase.
Drag the appropriate items to their respective bins.
can proofread using a 3'-to-5'
exonuclease activity
polymerize in a 5'-to-3' direction
Only RNA
can initiate strand synthesis
catalyze phosphodiester bond
formation to polymerize nucleotides
into nucleic acids
Only DNA
use deoxyribonucleotide triphosphates
as substrates
can only extend an existing strand
Both
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dependent on a DNA sequence
template
use ribonucleotide triphosphates as
substrates
Supercoiling of DNA
requires GTP as source of energy
is only observed in prokaryotes
is not observed in eukaryotes
requires the action of topoisomerases
Supercoiling of DNA
requires GTP as source of energy
is not observed in eukaryotes
requires the action of topoisomerases
is only observed in prokaryotes
Chapter 26 Solutions
Inquiry Into Life (16th Edition)
Ch. 26.1 - Describe the steps in forming Recombinant DNA.Ch. 26.1 - Discuss how the polymerase chain reaction works.Ch. 26.1 - Prob. 3LOCh. 26.1 - Prob. 1QTCCh. 26.1 - Prob. 2QTCCh. 26.1 - Prob. 1CYPCh. 26.1 - Explain how the PCR reaction amplifies a segment...Ch. 26.1 - Prob. 3CYPCh. 26.1 - Prob. 1AQTCCh. 26.1 - Prob. 2AQTC
Ch. 26.2 - Prob. 1LOCh. 26.2 - Prob. 2LOCh. 26.2 - Prob. 1QTCCh. 26.2 - Prob. 2QTCCh. 26.2 - Prob. 3QTCCh. 26.2 - Prob. 1CYPCh. 26.2 - Prob. 2CYPCh. 26.3 - Compare and contrast in vivo and ex vivo gene...Ch. 26.3 - Prob. 2LOCh. 26.3 - Prob. 1CYPCh. 26.3 - Prob. 2CYPCh. 26.3 - Prob. 3CYPCh. 26.4 - Prob. 1LOCh. 26.4 - Prob. 2LOCh. 26.4 - Prob. 3LOCh. 26.4 - Prob. 1CYPCh. 26.4 - Explain how comparative genomics can provide...Ch. 26.4 - Prob. 3CYPCh. 26 - Prob. S25.1BYBCh. 26 - Prob. S25.2BYBCh. 26 - Prob. S25.3BYBCh. 26 - Prob. 1ACh. 26 - Prob. 2ACh. 26 - The polymerase chain reaction Use RNA polymerase...Ch. 26 - Prob. 4ACh. 26 - Prob. 5ACh. 26 - In this process, cells are removed from the body...Ch. 26 - When a cloned gene is used to modify a human...Ch. 26 - Prob. 8ACh. 26 - Prob. 9ACh. 26 - Prob. 10ACh. 26 - Prob. 1TCCh. 26 - In a genomic comparison between humans and yeast,...
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- Why is the following statement fals? RNA polymerase is a dangerous enzyme to the cell because it does not proofread as well as DNA polymerasearrow_forwardPolymerases synthesize nucleic acids in the DNA and RNA is in the directinon. Fill in the two blanks with: 5' to 3'; 5' to 3' 3' to 5'; 5' to 3' O 5' to 3'; 3' to 5' 3' to 5'; 3' to 5' direction, and chemical synthesis ofarrow_forwardWhich of the following helped show that DNA is the carrier of genetic information? Check all that apply Heat-killed S bacteria can "transform" live R bacteria into a virulent strain. 20 different amino acids are found in proteins, but only 4 different nucleotides are found in DNA Bacteriophage inject DNA, not protein, into the bacteria they infect Mice injected with heat-killed R strains of bacteria die of pneumonia MacBook Proarrow_forward
- Polymerase chain reaction is— a reaction involving a chain of different types of polymerases acting successively on a DNA. a way DNA replicates in the living cell. governed by the Henderson-Hasselbalch equation. a chain reaction mediated by polymerase to amplify DNA fragments of defined sequence. DNA polymerization without a template.arrow_forwardWhich of the following features is common to both DNA replication and RNA transcription? Both RNA and DNA polymerase form a sugar-phosphate bond between the 3' hydroxyl and the 5' phosphate. Both RNA and DNA polymerase require oligonucleotide priming. Both RNA and DNA polymerase incorporate deoxyribonucleotides into the growing sequence. Both RNA and DNA polymerase initiate at promoter sequences. Both RNA and DNA polymerase have extensive proofreading editing.arrow_forwardMatch the proteins with their function in DNA replication. unwinds DNA at replication fork forms covalent phosphodiester bonds to join DNA fragments remove primer and replace the RNA with DNA, also has 3'-> 5'exonuclease activity for proofreading synthesizes DNA by adding to the 3' end of the growing strand of DNA adds RNA primer uses an RNA template to facilitate extension of the telomeres in eukaryotes introduces a double-stranded break ahead of the replication fork and swivels the cleaved ends around the central axis to relieve the stress of helix unwinding helicase [Choose ] ✓ helicase telomerase DNA polymerase II DNA polymerase I DNA polymerase III DNA primase DNA ligase D DNA Gyrase (topoiso- merase) exonucleasearrow_forward
- Match the proteins with their function in DNA replication. unwinds DNA at replication fork forms covalent phosphodiester bonds to join DNA fragments remove primer and replace the RNA with DNA, also has 3'-> 5'exonuclease activity for proofreading synthesizes DNA by adding to the 3' end of the growing strand of DNA adds RNA primer uses an RNA template to facilitate extension of the telomeres in eukaryotes helicase DNA ligase DNA polymerase II introduces a double-stranded break ahead of the replication fork and swivels the cleaved ends around the central axis to relieve the stress of helix unwinding DNA polymerase III DNA primase telomerase DNA Gyrase (topoisomerase)arrow_forwardWhich of the following are major differences between DNA and RNA polymerases? RNA polymerase, but not DNA polymerase, requires a primer to begin synthesizing the nucleic acid polymer DNA polymerase, but not RNA polymerase, requires a primer to begin synthesizing the nucleic acid polymer RNA polymerase, but not DNA polymerase, is unique to eukaryotic cells DNA polymerase, but not RNA polymerase, is unique to eukaryotic cells DNA polymerase is involved in replication; RNA polymerase is involved in transcription 00arrow_forwardsingle strand binding proteins are important for this activity prevent replication of the ends of the chromosomes prevent denaturation of the dsDNA template prevent the double helix from becoming a triple helix prevent single stranded DNA strands from rewinding and snapping back as double strandedarrow_forward
- Which of the following functions are associated with the E. coli RNA polymerase? Select all that apply Helicase activity Topoisomerase activity 3' to 5' polymerase activity 5' to 3' polymerase activityarrow_forwardFigure 4.10 Enzymes, temperature, and pH. Each enzyme works best within a characteristic range of conditionsgenerally the same environmental conditions in which the enzyme normally occurs. Figure It Out: At what temperature does the E.coli DNA polymerase work fastest?arrow_forwardWhich of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.arrow_forward
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