(a)
Interpretation:
Retention factors for each compound should be calculated.
Concept introduction:
Following equation can be used in calculation of retention factors.
(b)
Interpretation:
Distribution constants for each compound should be calculated.
Concept introduction:
The distribution constant can be calculated as follows:
(c)
Interpretation:
Selectivity factor for methylcyclohexane and methylcyclohexene should be calculated.
Concept introduction:
For the calculation of selectivity factor for methylcyclohexane and methylcyclohexene, the following formula will be used:
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Chapter 26 Solutions
Principles of Instrumental Analysis
- A 50.0 mL juice extract is colorimetrically assayed using Nelson’s test. One milliliter (1.00 mL) of the solution and the standard glucose solution (concentration: 1mg/mL) were treated with freshly prepared Nelson’s reagent and arsenomolybdate reagent and then diluted to 10.0 mL separately in properly labeled test tubes. Absorbance at 480 nm for the standard is 1.702 and for the sample, 0.926. A. What is the principle behind the assay? B. Calculate the concentration of the reducing sugar in the 50.0 mL sample in mg/mL. Apply Beer-Lambert’s law.arrow_forwardDuring an assay of paracetamol by UV an analyst prepares a series of calibration solutions containing approximately 3-15 mcg/mL paracetamol as follows: 140.1 mg of pure paracetamol was added to a 200 mL volumetric flask. 0.1 M NaOH (50 mL) was added, the mixture well shaken and made up to volume with deionized water 10 mL of the above solution was diluted to 100 mL 1)What is the concentration of the final diluted solution in mcg/mL?(1 decimal) 2) What volume of the diluted solution is required to prepare 100 mL of a 8 mcg/mL standard solution? (1 decimal)arrow_forwardSPECTROPHOTOMETRY 1.) Slope of regression line or Molar absorptivity (µM -1cm-1) is 0.0253 and absorbance of unknown solution is 0.131. What is the concentration of the unknown solution (µM )?arrow_forward
- ▪ In a blood alcohol analysis by GC-FID, methanol elutes at 1 min, ethanol at 2 min, IPA at 3 min, and IS at 4 min. In the patient sample, the analyte elutes t 2 min and the IS at 8 min. What is the relative retention time and the analyte in the patient? A) methanol B) Ethanol chu C) IPAarrow_forwardDuring an assay of paracetamol by UV an analyst prepares a series of calibration solutions containing approximately 3-15 mcg/mL paracetamol as follows: 135.4 mg of pure paracetamol was added to a 200 mL volumetric flask. 0.1 M NaOH (50 mL) was added, the mixture well shaken and made up to volume with deionized water • 10 mL of the above solution was diluted to 100 mL a) What is the concentration of the final diluted solution in mcg/mL? Answer = mcg/mL (1 decimal) tem b) What volume of the diluted solution is required to prepare 100 mL of a 5 mcg/mL standard solution? Answer = mL (1 decimal) A Quearrow_forward▪ In a blood alcohol analysis by GC-FID, methanol elutes at 1 min, ethanol at 2 min, IPA at 3 min, and IS at 4 min. In the patient sample, the analyte elutes t 2 min and the IS at 8 min. What is the relative retention time and the analyte in the patient? ▪ A) methanol B) Ethanol ▪ C) IPAarrow_forward
- Which compound has a retention time of 1.5 minutesarrow_forwardRetention times in a 1.25m chromatographic column: dead time, 0.395 min; isopropylamine, 4.59 min; and n-propylamine, 4.91 min.Peak widths: 0.365 min for isopropylamine and 0.382 min for n-propylamine. Calculate: (a) Value of the retention factor (k') for each amine (b) Value of the selectivity factor (alpha) between the two amines (c) Number of theoretical column trays (d) Column tray height (e) Resolution between the two aminesarrow_forward26. A mixture containing heptane, 2,2-dimethylbutane, hexanoic acid and heptanol was separated by gas/liquid chromatography. The stationary phase used was polar. In what order would the components leave the column? (A) (B) (D) Heptane, heptanol, hexanoic acid, 2,2-dimethylbutane Hexanoic acid, heptanol, heptane, 2,2-dimethylbutane 2, 2-dimethylbutane, heptane, heptanol, hexanoic acid Heptanol, 2, 2-dimethylbutane, hexanoic acid, heptanearrow_forward
- Below is the procedure described for determination of paracetamol according to BP: "Weigh and powder 20 tablets. Add a quantity of powder equivalent to about 0.35 g paracetamol to 50 ml of 0.1 M NaOH, dilute with water to 200 ml and shake for 15 min. Add sufficient water to make up 250 ml. Filter the solution and dilute 5 ml of the filtrate to 100 ml with water. Dilute further 10 ml to 100 ml. Measure the absorbance at 257 nm. The A," following is correct? 715 at 257 nm". Which of the O a. An analyst can take 0.37g of the powdered tablets instead of 0.35. O b. An analyst can replace NaOH with HCI OC. An analyst can skip the shaking step O d. B andC O e. All of the abovearrow_forwardBelow is the procedure described for determination of paracetamol according to BP: "Weigh and powder 20 tablets. Add a quantity of powder equivalent to about 0.35 g paracetamol to 50 ml of 0.1 M NaOH, dilute with water to 200 ml and shake for 15 min. Add sufficient water to make up 250 ml. Filter the solution and dilute 5 ml of the filtrate to 100 ml with water. Dilute further 10 ml to 100 ml. Measure the absorbance at 257 nm. The A, = 715 at 257 nm". Which of the following is correct? O a. An analyst can take 0.37g of the powdered tablets instead of 0.35. O b. An analyst can replace NaOH with HCl O c. An analyst can skip the shaking step O d. B andC O e. All of the abovearrow_forward3) What is the approximate retention time of benzoate?arrow_forward
- Principles of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage Learning