(a)
Interpretation:
The resolution for species B and C from the given data should be determined.
Concept introduction:
The resolution of the column is defined as the separation of two species of the column.
![Check Mark](/static/check-mark.png)
Answer to Problem 26.16QAP
The resolution is
Explanation of Solution
Given:
The expression of resolution of the column is:
Here, the retention time of species
Substitute
Thus, the resolution is
(b)
Interpretation:
The selectivity factor for species B and C from the given data should be determined.
Concept introduction:
The resolution of the column is defined as the separation of two species of the column.
![Check Mark](/static/check-mark.png)
Answer to Problem 26.16QAP
The selectivity factor is
Explanation of Solution
The expression of selectivity factor is:
Here, the non-retained retention time is
Substitute
Thus, the selectivity factor is
(c)
Interpretation:
The length of column necessary to separate B and C species with a resolution of
Concept introduction:
The resolution of the column is defined as the separation of two species of the column.
![Check Mark](/static/check-mark.png)
Answer to Problem 26.16QAP
The length of column necessary to separate the two species with a resolution of
Explanation of Solution
The expression of length of the column is:
Here, the number of plates needed to separate the two species is
The expression of relation of the resolution and number of plates is:
Here, the number of plates needed is
Substitute
Substitute
Thus, the length of column necessary to separate the two species with a resolution of
(d)
Interpretation:
The time required to separate B and C species on the column of part c should be determined.
Concept introduction:
The resolution of the column is defined as the separation of two species of the column.
![Check Mark](/static/check-mark.png)
Answer to Problem 26.16QAP
The time required to separate the two species on the column of part c is
Explanation of Solution
The expression of the relation of time required to separate the two species on the column is:
Here, the given resolution is
Substitute
Thus, the time required to separate the two species on the column of part c is
Want to see more full solutions like this?
Chapter 26 Solutions
PRINCIPLES OF INSTRUMENTAL ANALYSIS
- From the data in Problem 26-14, calculate for species C and D) (a) the resolution. (b) the length of column necessary to separate the two species with a resolution of 2.5.arrow_forward(a) You measure the percent transmittance of a solution containing chromophore X at 400nm in a 1-cm path length cuvette and find it to be 50%. What is the absorbance of this solution? (b) What is the molar absorptivity of chromophore X if the concentration of X in the solution measured in question (a) is 0.5mM? (c) What is the concentration range of chromophore X that can be assayed if, when using a sample cell of path length 1, you are required to keep the aborbance between 0.2 and 0.8?arrow_forwardTLC was used to compare the solubility of two compounds A and B in a solvent system (acetic acid, methanol, H2O). The distances that solvent has moved in the silica gel TLC was 12 cm. and for the compounds were 2 and 8 cm respectively. (a) Calculate the Rf values and which compound is more soluble and which one is more polar. (b) What are the factors affecting the Rf valuearrow_forward
- Substances A, B, C and D were found to have retention times of 8.3 min (A), 10.6 min (B), 12.4 min (C) and 13.5 min (D), respectively on an 15.0 cm long column. There was also an unretained species which appeared at 1.5 min. The widths of the peak bases were 0.90 min (A), 1.1 min (B), 1.3 min (C) and 0.80 min (D). Given this information listed above, please choose the most appropriate answer for the six questions listed below for the list of possible answers. Part A Calculate the capacity factor (k') for peak B and peak D. Part B. Calculate the resolution (R) between peaks B and D. Part C. What do the capacity factor and resolution tell you about the separation of peaks B and D on this column? Part D What are the theoretical plate number (N) for peaks Band D? Part E. What is the average plate height (H), based on the information in Part D and the column length listed above? Part F What would be the required length of a column to achieve a Rs of 2.07arrow_forwardThis method is to be used when measuring the volume of a solution containing sufficient reagent to react completely with the analyte. Volumetric O Spectroscopic Gravimetric Electroanalyticalarrow_forwardExactly 5.00 mL aliquots of a solution containing analyte X were transferred into 50.00-mL volumetric flasks and the pH of the solution is adjusted to 9.0. The following volumes of a standard solution containing 2.00 µg/mL of X were then added into each flask and the mixture was diluted to volume: 0.000, 0.500, 1.00, 1.50 and 2.00 mL. The fluorescence of each of these solutions was measured with a fluorometer, and the following values were obtained: 3.26, 4.80, 6.42, 8.02 and 9.56, respectively. ii. Using relevant functions in Excel, derive a least-squares equation for the data, and use the parameters of this equation to find the concentration of the phenobarbital in the unknown solution.arrow_forward
- A Dubosq colorimeter consists of a cell of fixed path length and a cell of variable path length. By adjusting the length of the latter until the transmission through the two cells is the same. the concentration of the second solution can be inferred from that of the former. Suppose that a plantdye of concentration 25 μg dm-3 is added to the fixed cell. the length of which is 1.55 em. Then a solution of the same dye. but of unknown concentration. is added to the second cel l. It is found that the same t ransmittance is obtained when the length of the second cel l is adjusted to 1.18 em. What is the concentration of the second solution?arrow_forwardQ4 (a) Analyze the importance of dropping mercury electrode on polarography. (b) (i) Calculate the molarity of MgSO4 in a solution containing 1.5 g ofMgSO4 in a volume of 250 mL?(ii) Calculate the weight of MgSO4 in grams which is present in 100mL of 0.076 M MgSO4?(c) Evaluate the key differences between paper chromatography and gaschromatographyarrow_forwardIn color chromatography of plant pigments, what complications would a dried-out column (solvent level is below the top of the silica) introduce to the elution and isolation of pigments?arrow_forward
- This method is to be used when measuring the volume of a solution containing sufficient reagent to react completely with the analyte. Group of answer choices Volumetric Spectroscopic Gravimetric Electroanalyticalarrow_forwardSubstances A and B have retention times of 16.4 and 17.63 min, respectively, on a 30 cm column. An unretained species passes through the column in 1.3 min. The peak width at base for A and B are 1.11 and 1.21 min, respectively. Calculate:arrow_forwardA molecular exclusion column has a diameter of 7.8 mm and a length of 30 cm. The solid portion of the particles occupies 20% of the volume, the pores occupy 40%, and the volume between particles occupies 40%. (a) At what volume would totally excluded molecules be expected to emerge? (b) At what volume would the smallest molecules be expected? (c) A mixture of polymers of various molecular masses is eluted between 23 and 27 mL. What does this imply about the retention mechanism for these solutes on the column?arrow_forward
- Principles of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage Learning
![Text book image](https://www.bartleby.com/isbn_cover_images/9781305577213/9781305577213_smallCoverImage.gif)