Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
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Chapter 24.1, Problem 1TYK
Summary Introduction
Introduction:
The microbiome consists of all the genetic material of the entire collection of microbes in a specific environment. Species of archaea, bacteria, protists and
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Jackson Wang is a biologist working with the genetics of a thermophilic bacterium. He cloned
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- Cloning vectors are not just limited to bacterial plasmids. Bacteriophages and M13 phage vectors are also commonly utilized in the cloning process. State any five (5) key criteria to be an effective cloning vector.arrow_forwardMuch of what we know about the human microbiome – the microbial communities associated with various parts of our bodies – comes from genome sequencing. Here, DNA is extracted from all cells in samples collected from various body sites and randomly sequenced, typically generating huge volumes of short-read sequencing data. These reads thus represent a random, mixed snapshot of genes encoded within any cell in the original sample. As human cells are commonly collected along with the bacteria, researchers first remove human DNA sequences from the dataset. To do this, they computationally remove any reads that are highly similar to the human reference genome; the remaining gene sequences are then assumed to belong to the microbial community. Researchers studying the human microbiome have frequently been interested to find that these bacterial communities seem to contain a number of highly unusual genes that are unique to human-associated microbes. a) Briefly describe one feature of…arrow_forwardThe following DNA sequence is from a bacteriophage that infects a pathogenic bacterium and scientists want to know if this bacteriophage could prove to be a potential treatment against it. But first scientists need to discover if different strains of this pathogen have restriction endonucleases that it may use for its own protection. They try 3 different RE’s:a) EcoR1 b) HaeIII c) BamH1 Look up the recognition sequences for the 3 Res. Enzymes above and check whether the phage genome (a snippet of which is shown below) will or will not be ‘cut’. Tell me how their experiment worked out and what their conclusion was.G A A A A G G C C A C A A G G C C G T C G A C T T T T A A A A G G C C A C A T G C G G C T T T T C C G G T G T T C C G G C AG C T GA A A AT T T T C C G G T G T A C G CCarrow_forward
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8C - How to do genetic analysis; Author: Useful Genetics;https://www.youtube.com/watch?v=HIa7nPyGn4s;License: CC-BY