Fundamentals of Biochemistry: Life at the Molecular Level
Fundamentals of Biochemistry: Life at the Molecular Level
5th Edition
ISBN: 9781118918401
Author: Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher: WILEY
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Chapter 24, Problem 12E
Summary Introduction

To explain: How the melting curve of duplex DNA is affected by adding a small amount of ethanol.

Concept introduction: Melting temperature (Tm) is the temperature at which half of the DNA melts or denatures, due to the break in the hydrogen bond that holds the two strands together. Melting temperature of a DNA duplex highly depends on the changes in the structure and the stability of the DNA. Non-polar solvents like ethanol and their addition decreases the melting point of DNA.

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