Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 21.9, Problem 1MI
How would you test whether or not Gld proteins are necessary for gliding motility? Explain the experiment you would perform and what your prediction would be.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Copy and paste the link below and watch the video on Youtube
https://www.youtube.com/watch?v=8RBs0Ghg_48
Answer the following Questions:
1. What are the chemicals and materials used in gel electrophoresis?
2. Draw a schematic diagram of a gel electrophoresis set-up
3. Describe the procedure in doing a gel electrophoresis experiment. Why is there a need for a leveling bubble/leveler? What is the use of the rubber dam?
4. What is the use of ethidium bromide and why must you wear gloves when you handle it?
5. What makes the DNA fragment move towards the positive plate?
6. What is the purpose of glycerol in the sample buffer?
7. What is the use of a DNA ladder?
8. What will happen when you increase the voltage of the set-up?
9. Can gel electrophoresis be used to separate amino acids? If so, how is it done?
In the context of flow cytometry data:
It is clear that Lactobacillus cells can be detected based on their FSC (forward scatter) and SSC (side scatter) signal since it is above the noise. Thus, they could be quantified using these parameters without staining. Give two reasons as to why anyone would bother SYBRGreen (SG) staining samples that contain heterotrophs?
You got an opportunity to join a professor lab who is working in-vivo model and specifically looking at the dysregulation of mitochondria in liver. He asked you to isolate mitochondria from a Rat liver and placed in an assay medium. Based on the knowledge you gain in this course so far, please answer the following questions:
a) Which technique will you use to isolate mitochondria?
b) What happens to the pH of the medium when the medium is kept anaerobic?
c) What happens when O2-saturated saline is added to the mixture?
Chapter 21 Solutions
Prescott's Microbiology
Ch. 21.3 - In what ways are members of Aquifex and Thermotoga...Ch. 21.3 - Prob. 2RIACh. 21.3 - Design an experiment designed to explore the role...Ch. 21.3 - Explain the relationship between genome size and...Ch. 21.4 - Why do you think NH3 is converted to the amino...Ch. 21.4 - Which of the phototrophs discussed perform...Ch. 21.4 - Prob. 1.2RIACh. 21.4 - Compare the capacity of green nonsulfur...Ch. 21.4 - Prob. 2.1RIACh. 21.4 - Prob. 2.2RIA
Ch. 21.4 - Prob. 2.3RIACh. 21.4 - Describe how a vegetative cell, a heterocyst, and...Ch. 21.4 - Prob. 2.5RIACh. 21.4 - Prob. 2.6RIACh. 21.5 - Prob. 1MICh. 21.7 - Prob. 1MICh. 21.7 - Prob. 1RIACh. 21.7 - Prob. 2RIACh. 21.7 - Prob. 3RIACh. 21.7 - Prob. 4RIACh. 21.7 - What might have been some of the culture...Ch. 21.9 - How would you test whether or not Gld proteins are...Ch. 21.9 - Prob. 1RIACh. 21.9 - Prob. 2RIACh. 21.9 - Prob. 3RIACh. 21.9 - Prob. 4RIACh. 21.9 - Why are cytophagas ecologically important?Ch. 21 - The cyanobacterium Anabaena grows well in liquid...Ch. 21 - Compare the structural and functional differences...Ch. 21 - Prob. 3CHICh. 21 - Some believe that an ancient planctomycete evolved...Ch. 21 - Prob. 5CHICh. 21 - In 2014 a team of Chinese and Czech...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- In an in vitro motility assay, a newly discovered motor protein is found to transport an attached bead along a microtubule away from the end where fluorescent labeled tubulin dimers assembled. Which of the following are true? Select all that apply The motor protein is transporting the bead towards the minus end The motor protein is transporting the bead towards the plus end The motor protein belongs to the dynein family The motor protein belongs to the kinesin family Oarrow_forwardBacillus subtilis is known to harbour proA gene which is expressed as protease. Protease is an industrially important enzyme but the yield from Bacillus is not satisfactory. What processes of genetic engineering can you conduct to increase the production of proA gene? Briefly discuss your vision on the context.arrow_forwardYou wish to centrifuge and pellet yeast cells using a centrifuge. The protocol says that you needto centrifuge the culture at 2,000xg for 10 minutes at room temperature. The rotor of yourcentrifuge has a maximum diameter of 25.4cm and the minimum diameter of 12.4cm. At whatrpm should you spin the centrifuge for pelleting the cells?arrow_forward
- What is the major difference between animal cell culture and plant cell culture.arrow_forwardWhich of the following would be best observed for a bacterial cell using phase-contrast microscopy? a. Motility of cells b. Bacterial nucleoid c. Cell wall d. Glycocalyxarrow_forwardIn the context of flow cytometry data: The Lactobacillus cells also show a clear increase in PerCP (red) fluorescence when stained with SYBRGreen (SG) compared to unstained cells. Why might this be the case? Think about the emission spectrum of chlorophyll and SG in combination with the dichroic mirrors (e.g. the ‘filters’ for the detectors).arrow_forward
- You are trying to track a motor protein dynein movement on a microtubule in a cell using GFP-tagged dynein (GFP- dynein). The motor proteins move very fast on the microtubules. Which type of microscopy will you choose to study this fast dynamic event? Two photon microscopy Total Internal Reflection (TIRF) microscopy O Point scanning confocal microscopy Spinning disc confocal microscopyarrow_forwardYou are viewing a bacterial cell that is 0.4 um in length and width using the 100X objective on your microscope with yellow light of 600 nm. 6. What is the limit of resolution? Express this value in nm and um. Will you be able to see an individual bacterial cell? Why or why not? a. b. 1001Tarrow_forwardA student was observing a filament of blue green bacteria under low power (60X). The diameter of the field is 2.4 mm. At this magnification, the student was able to count 40 cells in a row across the middle of the field of view. a. What would be the average length of each cell? b. If the student switched to high power (600X), about how many cells would be seenarrow_forward
- Identify the statments whether they are TRUE or FALSE 1. You can use low speed centrifuge to separate cells at 1,000 rpm (4ºC). 2. You can use high speed centrifuge to separate ribosomes at 15,000 rpm (4ºC)arrow_forwardUsing the microscope Answer the following questions as you work through this exercise: 1. How is the letter “e” on the slide oriented when you see it with the naked eye as you mount it on the stage (i.e., is it right side up or upside down)? 2. How is the letter “e” on the slide oriented when you see it under low or high power magnification? 3. What effect, if any, does the compound light microscope have on the orientation of the image?arrow_forwardDescribe in detail all the steps needed to determine whether an Unknown bacteria has a Gram-positive or Gram-negative cell wall using the Gram stain. (You can describe the method presented in the Exercise 3-6 Gram stain videos or in the Lab Manual.) 1. Start by describing the method of transferring the bacteria onto the microscope slide, then describe heat-fixing the emulsion of the Unknown bacteria. (Do not describe all the steps needed to perform aseptic technique. Just say “Bacteria was added aseptically.”) 2. Describe the Gram stain in detail. (This description should include all the stains, how long the stain should set on slide, and Genus and species names of bacteria you are using as CONTROLS.)arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
GCSE PE - ANTAGONISTIC MUSCLE ACTION - Anatomy and Physiology (Skeletal and Muscular System - 1.5); Author: igpe_complete;https://www.youtube.com/watch?v=6hm_9jQRoO4;License: Standard Youtube License