You are trying to track a motor protein dynein movement on a microtubule in a cell using GFP-tagged dynein (GFP- dynein). The motor proteins move very fast on the microtubules. Which type of microscopy will you choose to study this fast dynamic event? Two photon microscopy Total Internal Reflection (TIRF) microscopy O Point scanning confocal microscopy O Spinning disc confocal microscopy

What does parfocal mean? How does this affect the process of using a microscope? What could happen if your microscope is not parfocal?

When would you use a compound light microscope instead of a dissecting microscope?

In microscopy, why are different stains used for different samples? Be specific.

You are trying to look at a specimen with a compound microscope using the 4x objective, but it is very dark. Mention three parts of the microscope you could adjust that would help see your specimen in this darkness. Explain how you would adjust those parts.

Describe how the images obtained using a confocal microscope would appear different to those obtained using a widefield microscope.

what direction does a spiceman move on a microscope when you adjust stage X  to the left and right