Identify the statments whether they are TRUE or FALSE 1. You can use low speed centrifuge to separate cells at 1,000 rpm (4ºC). 2. You can use high speed centrifuge to separate ribosomes at 15,000 rpm (4ºC)
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Identify the statments whether they are TRUE or FALSE
1. You can use low speed centrifuge to separate cells at 1,000 rpm (4ºC).
2. You can use high speed centrifuge to separate ribosomes at 15,000 rpm (4ºC)
Step by step
Solved in 2 steps
- Beginning with 10 grams of plant tissue, order the following procedures (as steps 1-4) that you will use to obtain a cell fraction that consists of mostly large cell organelles: collect pellet, collect supernatant, high-speed centrifugation for 10 min., low-speed centrifugation for 5 min, filter homogenate, grind plant tissue Step 1 grind plant tissue | Choose collect supernatant low-spccd centrifugation for 5 min high-speed centrifugation for 10-min collect pellet grind plant tissue filter homogenate Step Step 3 Step 4 high-speed contrifugation fo vYou wish to centrifuge and pellet yeast cells using a centrifuge. The protocol says that you needto centrifuge the culture at 2,000xg for 10 minutes at room temperature. The rotor of yourcentrifuge has a maximum diameter of 25.4cm and the minimum diameter of 12.4cm. At whatrpm should you spin the centrifuge for pelleting the cells?In Figure 5-5,a. Why do A− and B− cells, by themselves, not formcolonies on the plating medium?b. What genetic event do the purple colonies in themiddle plate represent?
- Imagine you have been given a liquid culture of yeast with a starting concentration of 3.67 x 10' cells/ml and are asked to carry out the sample dilution process shown in the figure below. 100μl 100μl 100μl 100μl 100μl 0.9ml 0.9ml 0.9ml H2O H₂O 6.9ml 0.9ml H₂O H₂O H₂O Original 10-1 102 10-3 104 Culture 105 100μl 100μl 100μl Plate A Plate B Plate C a. How many colonies should have been present on Plate A in this example? - Answers must be whole numbers as partial colonies are not expected. b. Imagine you carried out the same dilution scheme shown in the figure above, but now, you do not know the concentration of the original culture. If you counted 163 colonies on Plate B, what is the concentration of cells/ml in the original culture?Three actively growing cultures of the same strain of yeast (saccharomyces cerevisiae) are used. Culture A has been incubated for 48 hours and Culture B has been incubated for 24hours and Culture C for 6 hours. a) Make a drawing of the cells from each culture to identify the various stages of the cell division. b) Is there any difference between the 3 cultures?The early steps of rabiesvirus (CVS) infection in vitro were studied in chicken embryo cells (CER) cells. The infection was monitored by looking for specific viral inclusions using anti- rabies fluorescein isothiocynanate at 24 hours after addition of virus. Cells were incubated for various lengths of time after virus attachment with ammonium chloride and chloroquine. The results are show in Figure 2. 100 75 s0 I 3 5 7 Time (h) 24 (m) 20 mM NH,CI (0) 0.1 mM chloroquine Figure 2 What is the effect of treatment of CER cells with chloroquine? Explain. Fluorescence inhibition (%)
- Density gradient centrifugation is an inexpensive cell separation technique. Mention 2 limitations of this separation techniqueIn lab we learned a technique that helped us to visulize individual colonies of bacter 1. Describe this technique. 2. What do you expect the resutls to look like? Be specific. 3. How can this technique help you to determine if your culture is contaminated? For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac). BIUS Paragraph I +] F H Ix X ABC † ( O K₂ KN Q V Arial sè "Ω Θ A 4 10pt EE 88 A Click Save and Submit to save and submit. Click Save All Answers to save all answers. 描く前 X² X₂ 3 由用目1. There are many ways you can accomplish a 1000-fold dilution. Propose 3 different serial dilution methods that will accomplish a dilution with a 1:1000 dilution factor. 2. Suppose you count 35 bacterial cells from a solution that has gone through the following serial dilutions: two 10-fold dilutions, followed by a 5-fold dilution, followed by 2-fold dilution. What should be the bacterial count from the original solution? Show your work! 3. When making a dilution you need to put different volumes of the stock solution and the buffer. Suppose you are doing a 2-fold dilution, where both volumes are the same. Will you use the same pipette tip to extract the stock and the buffer solution? Explain your reasoning.
- How does the microscopy in Figure 2 show that the capsule and an S-layer can exist in the same cell at the same time? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/You are doing a viable cell count for a culture of E. coli you are growing in a flask. The volume of media in the flask is 1L. You took an aliquot directly from the media and diluted it using trypan blue. Here are the numbers that you get: squares counted: 4 total cells counted: 988 total viable cells counted: 960 THESE ARE THE QUESTIONS: What is your cell viability? What is the total number of viable cells in your flask? Show all work. Given: Cell Viability = Viable Cells/Total Cells Cells/mL = Total Viable Cells/Coners Counted x 10,000 x Dilution factorAssume that you are starting with a sample containing 6 billion active cells. Create a dilution plan to reduce this count to a range of 30-300 colonies on a plate. The powdered sample weighs 500 mg (0.5 g). You can use up to 4 plates – you should plate different dilutions and/or volumes of solution. Start your first dilution by adding 10 mL diluent to 0.5 g powder, which is a 1/20 dilution. Hint: Rearranging the “cfu/ml” formula from our dilutions lab can help you solve this. Choose a target cfu count, and your volume plated should be under 0.5 ml.