Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 2, Problem 18RQ
Summary Introduction
To review:
Definition of the following terminologies:
(a) Cytoskeleton
(b) Microtubule
(c) MAP
(d) IFT
(e) Primary cilium
Introduction:
The components of the cytoskeleton include microfilaments, microtubule, snd intermediate fibers. Whip-like appendages, which are present over the plasma membran, ere referred to as flagella and cilia.
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Question 1.
Enzymes, proteins and deoxyribonucleic acid (DNA) are important biological
macromolecules. Enzymes are not only speed up the reaction, but also are necessary for DNA
reproduction.
c) Describe an example of biomedical application that utilizes a protein folding technique.
d) Describe the effect of pH on the oxygen binding affinity of hemoglobin.
Question #2
To isolate DNA from cells, you must disrupt the cell Therefore, you treated your cells with a lysis buffer that contains a detergent. You also heated the cells and vortexed them during this time.
What chemical property does the detergent in the lysis buffer have that helps disrupt a cell membrane? Briefly explain your answer.
How does vortexing and heating your samples aid in disrupting the membrane?
The purpose of mitosis in human cells is for the production of two diploid, _________________ cells.
Chapter 2 Solutions
Biochemistry: The Molecular Basis of Life
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