Campbell Biology
Campbell Biology
12th Edition
ISBN: 9780135188743
Author: Urry
Publisher: PEARSON
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Chapter 19.2, Problem 5CC
Summary Introduction

To propose a hypothesis: To identify ways to block the molecular pathway to fight HIV infection.

Concept introduction: HIV is a retrovirus having RNA as a genetic material. HIV mainly attacks the immune cells, especially CD4 cells. CD4 cells play an essential role by protecting the body from any infection. Antiretroviral therapy is a method of suppressing or preventing HIV infection using drugs, e.g., Tenofovir, Zidovudine.

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How will the information required to describe your RFP be obtained? Which kinds of methods are you going to employ?    lab intro attached if needed
PART C: CALCULATING THE DIAMETER OF THE FIELD OF VIEW (FOV)_ The field of view (FOV) is the circular area you can see when you look through the microscope. The diameter of the field of view is different depending on which objective lens you are using. For example, you are using the medium-power objective lens, then the area you can see is actually smaller than if you were using the low-power objective lens. Knowing The diameter of the field of view can help you estimate actual size of objects / cells seen through the microscope. When the revolving nose piece is turned to the low power objective lens, a dear plastic ruler can be placed on the microscope stage (see figure 1). Then, the coarse adjustment knob can be used to focus on the millimeter marks of the ruler making sure that one of the milimeter marks is at the left edge of the field of view (see figure 2). NOTE: Slage cip I-1000 Objects in the FOV are usually measured in micrometers (um). To convert, a FOV in mm, times it by 1000…
Horizontal sequence :VIRL Vertical sequence:MKF   Scoring rules: g/o = -3, g/e = -1, match or mismatch - from PAM250 substitution matrix below.    SW algorithm.    1. Complete the scoring matrix.    Scoring matrix with PAM250 scores:     V I R L M         K         F           2. Set up, initialize and complete the SW matrix.  3. Retrace, align and score alignment(s).             Use the arrows and circles for the matrix and path(s).                                                                                                                                                                                                                                                                                                                                 V I R L             M           K           F             Align and score all optimal alignments here.
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