Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 18.7, Problem 1MI
Summary Introduction
Comparative genomics plays an important role in discerning the adaptive nature of the microbe to the specific ecological niches and in the development of new therapeutic agents. Comparing the genomes reveals the information about the evolution and the function of genes. This includes the importance of horizontal gene transfer (HGT). The comparative genomics focuses on the similar amino acid sequences and
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In E. coli, the genes for histidine (his), arginine (arg), tyrosine (tyr), and valine (val) synthesis are closely linked. A wild-type strain is used as a donor for transducing viruses. Various recipient strains were treated with these viruses. Wild-type recombinant colonies were then counted, with the results shown in the following table.
Genotype of recipient
Number of wild-type colonies
Genotype of recipient
Number of wild-type colonies
his- arg+ val+ tyr+
254
his+ arg- val- tyr+
132
his+ arg+ val- tyr-
36
his+ arg- val+ tyr+
240
his- arg- val+ tyr+
28
his- arg+ val- tyr+
102
his- arg+ val+ tyr-
100
his+ arg- val+ tyr-
2
What is the cotransduction coefficient of histidine and valine? For consistency, standardize on histidine.
0.38 (wrong)
0.537
0.402
None of these
Consider the five E. coli merodiploid strains listed here.
Strain #1 I+P+O+Z-Y+/I+P+OcZ+Y+
Strain #2 I+P+O+Z+Y+/I-P+OcZ+Y-
Strain #3 I+P+O+Z-Y+/I-P+OcZ+Y-
Strain #4 I-P-O+Z+Y-/I+P+OcZ-Y+
Strain #5: ISP+O+Z+Y+/I-P+O+Z+Y-
Which of these strains will be unable to express lactose permease under any conditions? Select all correct answers.
A.)Strain #3
B.)Strain #4
C.)Strain #2
D.)Strain #5
E.)Strain #1
A graduate student was assaying LD50 (lethal dose 50%) of two temperature-sensitive
Francisella tularensis strains in HeLa cells (human cell line). Both strains can infect humans
and cause fatal tularemia if untreated, but it is difficult to obtain LD50 values in human
subjects. The data below shows LD50 (lethal dose 50%) values of the strains in human cell
culture. Can you predict the more virulent strain of the two human pathogens?
Francisella tularensis strain A: LD50 @ 20°C= 100; LD50 @ 37°C= 1000
Francisella tularensis strain B: LD50 @ 20°C= 1000 LD 50 @ 37°C= 100
O It is not possible to determine the virulence of the two strains as human pathogens from the
provided data
Strain A and strain B are equally virulent as human pathogens, as they average out in virulence.
O Strain A is more virulent than strain A as a human pathogen.
O Strain B is more virulent than strain A as a human pathogen.
Chapter 18 Solutions
Prescott's Microbiology
Ch. 18.1 - MICRO INQUIRY What is the function of the 3-OH...Ch. 18.1 - MICRO INQUIRY Why is it important that identical...Ch. 18.2 - MICRO INQUIRY Which step (or steps) in this...Ch. 18.2 - Retrieve, Infer, Apply Why is the Sanger technique...Ch. 18.2 - Retrieve, Infer, Apply Explain the difference...Ch. 18.2 - Retrieve, Infer, Apply Why does reversible chain...Ch. 18.2 - Prob. 4RIACh. 18.2 - Retrieve, Infer, Apply Suggest a medical and an...Ch. 18.3 - Retrieve, Infer, Apply What is a phylotype?Ch. 18.3 - Retrieve, Infer, Apply NGS techniques are...
Ch. 18.3 - Retrieve, Infer, Apply Examine figure 18.8. How...Ch. 18.4 - Prob. 1MICh. 18.5 - Figure 18.12 Metabolic Pathways and Transport...Ch. 18.5 - Prob. 2MICh. 18.5 - Prob. 3MICh. 18.5 - Prob. 1.1RIACh. 18.5 - Retrieve, Infer, Apply How might the following...Ch. 18.5 - Retrieve, Infer, Apply What specific limitations...Ch. 18.5 - Retrieve, Infer, Apply Compare and contrast...Ch. 18.5 - Retrieve, Infer, Apply Why does two-dimensional...Ch. 18.5 - Retrieve, Infer, Apply What is the difference...Ch. 18.5 - Retrieve, Infer, Apply Describe a ChIP-Seq...Ch. 18.7 - Prob. 1MICh. 18.7 - Retrieve, Infer, Apply Cite an infectious disease...Ch. 18.7 - Prob. 2RIACh. 18.7 - Prob. 3RIACh. 18 - Propose an experiment that can be done easily with...Ch. 18 - Prob. 2CHICh. 18 - You are developing a new vaccine for a pathogen....Ch. 18 - Prob. 4CHICh. 18 - Prob. 5CHICh. 18 - Prob. 6CHI
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