Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 18.5, Problem 3MI
Summary Introduction
In tandem mass spectrometry, the collection of protein fragments is run through the two mass spectrometers in a sequential manner. This forms the difference between the mass spectrometry and tandem mass spectrometry. In this method, the first spectrometer separates the proteins and fragments which are to be fragmented further. Then, the second spectrometer determines the amino acid sequence of each of the smaller fragments on the basis of the molecular weight. Thus, this technique uses two mass analyzers that are combined in series in order to select the protein.
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Suppose that the sequence is part of a larger globular protein.
Consider the probable location, either on the external surface
or the interior of the protein, of each of the amino
acid residues.
The table gives the hydropathy index values for selected
amino acids in the peptide.
Amino acid
Hydropathy index
alanine
1.8
isoleucine
4.5
threonine
-0.7
glutamine
-3.5
lysine
-3.9
aspartate
-3.5
How long is a fully extended peptide chain that contains the same number of amino acids?(The distance between consecutive amino acids in a fully extended chain is 3.5 Å; the repeat distance ofan a-helix is 5.4 Å.)
Assume you were given a mixture consisting of one molecule each of all possible sequences of a smallish protein of molecular weight 4800 daltons. If the average molecular weight of an amino acid is, say, 120 daltons, how much would the sample weigh? How big a container would you need to hold it?
Chapter 18 Solutions
Prescott's Microbiology
Ch. 18.1 - MICRO INQUIRY What is the function of the 3-OH...Ch. 18.1 - MICRO INQUIRY Why is it important that identical...Ch. 18.2 - MICRO INQUIRY Which step (or steps) in this...Ch. 18.2 - Retrieve, Infer, Apply Why is the Sanger technique...Ch. 18.2 - Retrieve, Infer, Apply Explain the difference...Ch. 18.2 - Retrieve, Infer, Apply Why does reversible chain...Ch. 18.2 - Prob. 4CCCh. 18.2 - Retrieve, Infer, Apply Suggest a medical and an...Ch. 18.3 - Retrieve, Infer, Apply NGS techniques are...Ch. 18.3 - Retrieve, Infer, Apply Examine figure 18.8. How...
Ch. 18.4 - Prob. 1MICh. 18.4 - Prob. 1CCCh. 18.4 - Prob. 2CCCh. 18.4 - Prob. 3CCCh. 18.5 - Figure 18.12 Metabolic Pathways and Transport...Ch. 18.5 - Prob. 2MICh. 18.5 - Prob. 3MICh. 18.5 - Prob. 1CCCh. 18.5 - Retrieve, Infer, Apply How might the following...Ch. 18.5 - Retrieve, Infer, Apply Compare and contrast...Ch. 18.5 - Retrieve, Infer, Apply Why does two-dimensional...Ch. 18.5 - Retrieve, Infer, Apply What is the difference...Ch. 18.5 - Retrieve, Infer, Apply Describe a ChIP-Seq...Ch. 18.7 - Prob. 1MICh. 18.7 - Retrieve, Infer, Apply Cite an infectious disease...Ch. 18.7 - Prob. 2CCCh. 18.7 - Prob. 3CCCh. 18 - Prob. 1RCCh. 18 - Prob. 2RCCh. 18 - Prob. 3RCCh. 18 - Prob. 4RCCh. 18 - Prob. 5RCCh. 18 - Prob. 1ALCh. 18 - Prob. 2ALCh. 18 - You are developing a new vaccine for a pathogen....Ch. 18 - Prob. 4AL
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