Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Question
Chapter 18.5, Problem 2MI
Summary Introduction
The microarray experiments produce a vast amount of information and it must be organized. One such approach is the use of hierarchical cluster analysis. This analysis groups the genes on the basis of its function and level of expression. The upregulated genes are indicated as red-colored spots and are grouped distinctly from the downregulated genes indicated as green colored spots. The unaltered gene expressions are indicated in black spots.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
The following table lists 4 bacterial strains that are partial diploids for lac operon genes. Given the
activity of beta-galactosidase measured for each strain in the absence (-lac) or presence (+lac) of
lactose, complete the table by choosing the appropriate symbol (+, -, C, S) to indicate the allele of
the gene or site missing from the table (blue numbers).
+ = wildtype, - = null mutation, c = constitutive, s =super repressor
chromosome
plasmid
B-gal act.
strain
10
Z
A
1 C
B
3
4
+
C
+
6
D
9
+
1
[Select]
3 [Select]
5 [Select]
7
[ Select]
9 [Select]
| 0
2
+
+ 5
+
7
10
Z
+
-lac
+lac
0.062 0.058
0.003
0.004
0.062 0.117
0.003 0.060
+
8
+
2 [Select]
4 [Select]
6 [Select]
8 [Select]
10
[Select]
Zoey Wong is a research officer at the Department of Biosciences of Tunku Abdul Rahman
University College. Her supervisor instructs her to prepare chemically competent cells for
heat shock transformation from old batches of competent cells that are already available in the
freezers. The competent cells will eventually be used for the expression of a prokaryotic
enzyme using the pET vector system.
(i)
(ii)
You found several different strains of Escherichia coli in the -80 C freezer. State
three (3) E. coli strains you would use for your project which involves the cloning
and expression of the protein of interest.
In order for cloning and expression to happen optimally, suggest two (2) types of pET
vectors suitable for your project.
https://ny.pbslearningmedia.org/resource/biot09.sci.life.gen.epigenetics/epigenetics/
In what way do the brown and yellow mice shown in the video differ?Why is this so?
Chapter 18 Solutions
Prescott's Microbiology
Ch. 18.1 - MICRO INQUIRY What is the function of the 3-OH...Ch. 18.1 - MICRO INQUIRY Why is it important that identical...Ch. 18.2 - MICRO INQUIRY Which step (or steps) in this...Ch. 18.2 - Retrieve, Infer, Apply Why is the Sanger technique...Ch. 18.2 - Retrieve, Infer, Apply Explain the difference...Ch. 18.2 - Retrieve, Infer, Apply Why does reversible chain...Ch. 18.2 - Prob. 4CCCh. 18.2 - Retrieve, Infer, Apply Suggest a medical and an...Ch. 18.3 - Retrieve, Infer, Apply NGS techniques are...Ch. 18.3 - Retrieve, Infer, Apply Examine figure 18.8. How...
Ch. 18.4 - Prob. 1MICh. 18.4 - Prob. 1CCCh. 18.4 - Prob. 2CCCh. 18.4 - Prob. 3CCCh. 18.5 - Figure 18.12 Metabolic Pathways and Transport...Ch. 18.5 - Prob. 2MICh. 18.5 - Prob. 3MICh. 18.5 - Prob. 1CCCh. 18.5 - Retrieve, Infer, Apply How might the following...Ch. 18.5 - Retrieve, Infer, Apply Compare and contrast...Ch. 18.5 - Retrieve, Infer, Apply Why does two-dimensional...Ch. 18.5 - Retrieve, Infer, Apply What is the difference...Ch. 18.5 - Retrieve, Infer, Apply Describe a ChIP-Seq...Ch. 18.7 - Prob. 1MICh. 18.7 - Retrieve, Infer, Apply Cite an infectious disease...Ch. 18.7 - Prob. 2CCCh. 18.7 - Prob. 3CCCh. 18 - Prob. 1RCCh. 18 - Prob. 2RCCh. 18 - Prob. 3RCCh. 18 - Prob. 4RCCh. 18 - Prob. 5RCCh. 18 - Prob. 1ALCh. 18 - Prob. 2ALCh. 18 - You are developing a new vaccine for a pathogen....Ch. 18 - Prob. 4AL
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Consider the following 4 regulatory pathways (recall arrows are activators and blunt ends are inhibitors). 01 2 3 1 A- 4 2 B- 3 C- 4 D E F G H T1 I Which of the following pathways would result in the final gene (I, J, K or L) being activated by the presence of the first gene (A, B, C, or D)? Select all that apply. J K Larrow_forwardBefore inserting the blunt-ended PCR amplicon to a dephosphorylated vector, the amplicon is treated with kinase (which adds phosphate groups). What is the rationale behind this kinase step?arrow_forwardYou have discovered a new bHLH gene and would like to use ChIP-Seq to learn more about it's role in gene regulation. Describe the experiment you would perform, include your expected results.arrow_forward
- Termicin is a small antifungal protein in termites that is produced by cells and secreted into termite saliva in response to a pathogen. In vitro translation of the termicin-encoding gene is performed, and the effects of that product are compared to those of termicin extracted from a termite. You see that extracted termicin exhibits more antifungal behavior than in vitro translated termicin. After further analysis, you see that extracted termicin contains 3 disulfide bonds, while in vitro translated termicin contains zero. The addition of microsomes to the in vitro translation reaction results in termicin with all 3 disulfide bonds. What experimental condition is most likely responsible for this difference? A. in vitro translation was not performed at the correct temperature affecting protein folding B. a mutation occurred during in vitro translation, leading to differences in disulfide bond formation O C. the UPR can not be activated in vitro, therefore, this protein can only be…arrow_forwardFor the lac genotypes shown in the following table, predict whether the structural genes (Z) are constitutive, permanently repressed, or inducible in the presence of lactose. Genotype Constitutive Repressed Inducible I+O+Z+ * I-O+Z+ I-OCZ+ I-OCZ+/F=O+ I+OCZ+/F=O+ ISO+Z+ ISO+Z+/F=I+arrow_forwardVarious kinds of biochemical events may explain why some disease alleles are dominant?arrow_forward
- The chart below is a position specific scoring matrix (PSSM, a logarithmic transformed matrix) for a transcription factor binding site. (1). Evaluate a sequence “GACATTCA” to find out which segment of the sequence fits the binding site best. (2) What is the max score that a sequence can have with this PSSM? (3) What is the minimum score a sequence can have with this PSSM?arrow_forwardBased on the given scenario, tell whether structural genes are expressed (YES) or not expressed (NO) 1. LacY is deleted (YES/NO) 2. LacI is deleted (YES/NO) 3. TrpR is deleted (YES/NO) 4. Promoter sequence is deleted (YES/NO) 5. lactose is present in the environment; glucose is absent (YES/NO) 6. tryptophan is absent in the environment (YES/NO) 7. operator is deleted (YES/NO)arrow_forwardYou have isolated different mutants (reg1 and reg2) causing constitutive expression of the emu operon (which has genes emu1 and emu2). One mutant contains a defect in a DNA-binding site, and the other has a loss-of-function defect in the gene encoding a protein that binds to the site Say you don’t know which mutant has a defect in the site and which one has a mutation in the binding protein. To figure it out, you construct the two partial diploid strains (i and ii below), and you then assay the levels of the Emu1 and Emu2 proteins in these two strains. F’ (reg1- reg2+ emu1- emu2+) / reg1+ reg2+ emu1+ emu2- F’ (reg1+ reg2- emu1- emu2+) / reg1+ reg2+ emu1+ emu2- What proteins do you predict will be expressed for strains i and ii if reg2 encodes the regulatory protein and reg1 is the regulatory site?arrow_forward
- Help me pleasearrow_forwardA number of mutations affect the expression of the lac operon in E. coli. Consider each genotype below and complete the table using “+” to indicate that the gene is expressed, and “−” to indicate that gene is not expressed.arrow_forwardAnswer choices are either "high, low" or "yes, no"; For the question: "what is the level of transcription?" the answer choices are "high, low, very low"arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
QCE Biology: Introduction to Gene Expression; Author: Atomi;https://www.youtube.com/watch?v=a7hydUtCIJk;License: Standard YouTube License, CC-BY