Whether added glucose in isolated mitochondria suspension is metabolized into H 2 O and CO 2. Introduction : Isotope labeling is a technique which is used to locate the path of an isotope in a metabolic pathway or reaction. In this technique, the reactant substrate is labeled. The labeling refers to the replacement of specific atom by their isotopes.

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Answer 1

Question

Chapter 16, Problem 16P

(a)

Summary Introduction

To determine: Whether added glucose in isolated mitochondria suspension is metabolized into H₂O and CO_2.

Introduction:

Isotope labeling is a technique which is used to locate the path of an isotope in a metabolic pathway or reaction. In this technique, the reactant substrate is labeled. The labeling refers to the replacement of specific atom by their isotopes.

(b)

Summary Introduction

To determine: The location of ¹⁴C in the oxaloacetate after one turn of citric acid cycle when a brief pulse of [3-¹⁴C] pyruvate is added into mitochondria. Also determine the number of turns of cycle required to release all the [3-¹⁴C] pyruvate as CO₂.

Introduction:

The series of metabolic reactions in which the energy stored in the compounds is released for the production of ATP (adenosine triphosphate), is called “Krebs’s cycle or citric acid cycle”.

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Answer 2

Question

Chapter 16, Problem 16P

(a)

Summary Introduction

To determine: Whether added glucose in isolated mitochondria suspension is metabolized into H₂O and CO_2.

Introduction:

Isotope labeling is a technique which is used to locate the path of an isotope in a metabolic pathway or reaction. In this technique, the reactant substrate is labeled. The labeling refers to the replacement of specific atom by their isotopes.

(b)

Summary Introduction

To determine: The location of ¹⁴C in the oxaloacetate after one turn of citric acid cycle when a brief pulse of [3-¹⁴C] pyruvate is added into mitochondria. Also determine the number of turns of cycle required to release all the [3-¹⁴C] pyruvate as CO₂.

Introduction:

The series of metabolic reactions in which the energy stored in the compounds is released for the production of ATP (adenosine triphosphate), is called “Krebs’s cycle or citric acid cycle”.

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Answer 3

Question

Chapter 16, Problem 16P

(a)

Summary Introduction

To determine: Whether added glucose in isolated mitochondria suspension is metabolized into H₂O and CO_2.

Introduction:

Isotope labeling is a technique which is used to locate the path of an isotope in a metabolic pathway or reaction. In this technique, the reactant substrate is labeled. The labeling refers to the replacement of specific atom by their isotopes.

(b)

Summary Introduction

To determine: The location of ¹⁴C in the oxaloacetate after one turn of citric acid cycle when a brief pulse of [3-¹⁴C] pyruvate is added into mitochondria. Also determine the number of turns of cycle required to release all the [3-¹⁴C] pyruvate as CO₂.

Introduction:

The series of metabolic reactions in which the energy stored in the compounds is released for the production of ATP (adenosine triphosphate), is called “Krebs’s cycle or citric acid cycle”.

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Answer 4

Question

Chapter 16, Problem 16P

(a)

Summary Introduction

To determine: Whether added glucose in isolated mitochondria suspension is metabolized into H₂O and CO_2.

Introduction:

Isotope labeling is a technique which is used to locate the path of an isotope in a metabolic pathway or reaction. In this technique, the reactant substrate is labeled. The labeling refers to the replacement of specific atom by their isotopes.

(b)

Summary Introduction

To determine: The location of ¹⁴C in the oxaloacetate after one turn of citric acid cycle when a brief pulse of [3-¹⁴C] pyruvate is added into mitochondria. Also determine the number of turns of cycle required to release all the [3-¹⁴C] pyruvate as CO₂.

Introduction:

The series of metabolic reactions in which the energy stored in the compounds is released for the production of ATP (adenosine triphosphate), is called “Krebs’s cycle or citric acid cycle”.

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Answer 5

Chapter 16, Problem 16P

(a)

Summary Introduction

To determine: Whether added glucose in isolated mitochondria suspension is metabolized into H₂O and CO_2.

Introduction:

Isotope labeling is a technique which is used to locate the path of an isotope in a metabolic pathway or reaction. In this technique, the reactant substrate is labeled. The labeling refers to the replacement of specific atom by their isotopes.

(b)

Summary Introduction

To determine: The location of ¹⁴C in the oxaloacetate after one turn of citric acid cycle when a brief pulse of [3-¹⁴C] pyruvate is added into mitochondria. Also determine the number of turns of cycle required to release all the [3-¹⁴C] pyruvate as CO₂.

Introduction:

The series of metabolic reactions in which the energy stored in the compounds is released for the production of ATP (adenosine triphosphate), is called “Krebs’s cycle or citric acid cycle”.

Answer 6

Chapter 16, Problem 16P

(a)

Summary Introduction

To determine: Whether added glucose in isolated mitochondria suspension is metabolized into H₂O and CO_2.

Introduction:

Isotope labeling is a technique which is used to locate the path of an isotope in a metabolic pathway or reaction. In this technique, the reactant substrate is labeled. The labeling refers to the replacement of specific atom by their isotopes.

Answer 7

Chapter 16, Problem 16P

(a)

Summary Introduction

To determine: Whether added glucose in isolated mitochondria suspension is metabolized into H₂O and CO_2.

Introduction:

Isotope labeling is a technique which is used to locate the path of an isotope in a metabolic pathway or reaction. In this technique, the reactant substrate is labeled. The labeling refers to the replacement of specific atom by their isotopes.

Answer 8

(b)

Summary Introduction

To determine: The location of ¹⁴C in the oxaloacetate after one turn of citric acid cycle when a brief pulse of [3-¹⁴C] pyruvate is added into mitochondria. Also determine the number of turns of cycle required to release all the [3-¹⁴C] pyruvate as CO₂.

Introduction:

The series of metabolic reactions in which the energy stored in the compounds is released for the production of ATP (adenosine triphosphate), is called “Krebs’s cycle or citric acid cycle”.

Answer 9

(b)

Summary Introduction

To determine: The location of ¹⁴C in the oxaloacetate after one turn of citric acid cycle when a brief pulse of [3-¹⁴C] pyruvate is added into mitochondria. Also determine the number of turns of cycle required to release all the [3-¹⁴C] pyruvate as CO₂.

Introduction:

The series of metabolic reactions in which the energy stored in the compounds is released for the production of ATP (adenosine triphosphate), is called “Krebs’s cycle or citric acid cycle”.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 16 - Prob. 1P Ch. 16 - Prob. 2P Ch. 16 - Prob. 3P Ch. 16 - Prob. 4P Ch. 16 - Prob. 5P Ch. 16 - Prob. 6P Ch. 16 - Prob. 7P Ch. 16 - Prob. 8P Ch. 16 - Prob. 9P Ch. 16 - Prob. 10P

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