Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 15.2, Problem 2CC
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Aside from gel electrophoresis Give another method to quantify DNA. Explain the concept behind this method.
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Chapter 15 Solutions
Prescott's Microbiology
Ch. 15.2 - Prob. 1CCCh. 15.2 - Prob. 2CCCh. 15.2 - Prob. 3CCCh. 15.2 - Prob. 4CCCh. 15.2 - Prob. 5CCCh. 15.3 - What functions are served by the 5 cap and the 3...Ch. 15.3 - Retrieve, Infer, Apply What elements in archaeal...Ch. 15.3 - Prob. 2CCCh. 15.3 - Prob. 3CCCh. 15.4 - Prob. 1CC
Ch. 15.4 - Prob. 2CCCh. 15.4 - Prob. 3CCCh. 15.5 - Retrieve, Infer, Apply List two similarities and...Ch. 15.5 - Prob. 2CCCh. 15.5 - Retrieve, Infer, Apply How are cis-encoded RNAs,...Ch. 15 - Prob. 1RCCh. 15 - Prob. 2RCCh. 15 - Prob. 3RCCh. 15 - Prob. 4RCCh. 15 - Prob. 5RCCh. 15 - Prob. 6RCCh. 15 - Prob. 7RCCh. 15 - Prob. 8RCCh. 15 - Prob. 9RCCh. 15 - Prob. 1ALCh. 15 - All of the subunits in bacterial RNA polymerases...Ch. 15 - Would you expect that one day microbiologists...Ch. 15 - In the chapter opening story, it was stated that...Ch. 15 - Prob. 5AL
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- List the steps in the polymerase chain reaction; discuss one disadvantage to this techniquearrow_forwardDiagram and explain how APEX probes can be used to determine that an individual is CC (homozygous) for a specific G/C SNV. Recall that the genotype of an SNV is identified from the strand shown in NCBI. What color fluorescence will be observed? Also, explain why a left apex probe cannot be used for this SNV. The SNV sequence, on the strand shown in NCBI, and a few nucleotides adjacent to the SNV are below: 5'-------TGT(G/C)CAG------3'arrow_forwardWhat are nanopores used for and tell How does DNA bind to the nanopore?arrow_forward
- Expand PCR? Describe the different Steps involved in this technique?arrow_forwardDescribe the proofreading activity of DNA polymerase.arrow_forwardElaborate on the basic principle you would adopt to isolate an amplified DNA fragment using agarose gel during electrophoresis. In you answer, provide factors that would facilitate migrationof the DNA fragment though the gel.arrow_forward
- Recall the semi discontinuity in DNA replication. Is it biologically possible for DNA to undergo replication in vivo, without the lagging and the leading strands? In PCR, we also start with an antiparallel strand of DNA. Does this also mean that replication is semidiscontinuous in in vitro replication? PLEASE EXPLAIN COMPLETELY AND NOT JUST COPY PASTE ANSWERS FROM GOOGLE.arrow_forwardDraw the gel electrophoretic pattern that would be seen in dideoxy sequenceanalysis of the DNA moleculearrow_forwardGive typed full explanation you made a recombinant plasmid (it is circular) that contains the sequence 5' AAGCTT in the middle of the inserted or cloned gene. that is the only place where this sequence occurs. the entire plasmif does not have 5' GAATTC. When putting this plasmid into E. coli (transformstion) what will happen? 1. plasmid will cut into several fragments 2. nothing the plasmif with remain circular 3. plasmid will be cut once making a linear dna fragment 4. will be cut once but will becomr two linear fragmentsarrow_forward
- Restriction endonuclease digestion of a DNA sequence yielded fragments of the following sizes: 1. 5.2 kb 2. 0.8 kb 3. 1.2 kb 4. 3.8 kb 5. 3.1 kb After gel electrophoresis, what would be the order in which these fragments would be found—the last fragment listed being furthest from the negative pole.arrow_forwardWhat is dideoxy sequencing? Explain it please.arrow_forwardCompare and contrast how the lagging strand is replicated in E. coli and in eukaryotes. Highlight the steps that are the same or similar, as well as the aspects of lagging strand synthesis that differ.arrow_forward
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