Prescott's Microbiology
Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 15, Problem 1AL
Summary Introduction

Telomerase is an enzyme that is responsible for the addition of telomeric units or repeats to the end of the bacterial chromosome. This enzyme is critical for maintaining the length of the telomere. Mammalian somatic cells lack the activity of this enzyme and therefore, results in the shortening of telomeres in each of the cellular respiration processes.

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You are interested in studying resistance to heavy metals and have selected the yeast Saccharomyces cerevisea to conduct your studies. You have recovered a deletion mutant that does not tolerate high concentrations of zinc (grows poorly in zinc containing media ) and have designated the mutant pgz-1 (for poor growth in zinc ). (a) What is the advantage to the type of mutant used in this work? What class of mutagen was likely use to generate pgz-1? ( b) Do you expect the PGZ gene to be expressed in your mutant? Explain.
Telomerase activity has been found to be 10 to 20 times more active in cancer cells than in normal somatic cells. What is the significance of this circumstance?
In the Inhibition of telomerase activity. Explain: (a) What is the process affected? (b) What is the Effect on the process? (c) Does it affect prokaryotes, eukaryotes or both?
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