BIOLOGY >PRINT UPGRADE<
11th Edition
ISBN: 9780357091586
Author: Solomon
Publisher: CENGAGE L
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Chapter 15, Problem 5TYU
Summary Introduction
Concept introduction: Polymerase chain reaction (PCR) is a process of amplification of a specific DNA sequence consisting of the gene of interest. It is a DNA-based technique. This makes many copies of the target DNA sequence and is done in vitro.
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(iii)
What are the three (3) main cycles in PCR?
(iv) Discuss the processes at each PCR cycle mentioned in Q3 a) (iii).
Which technique rapidly replicates specific DNA fragments without cloning in cells? (a) gel electrophoresis (b) cDNA libraries (c) DNA probe (d) restriction fragment length polymorphism (e) polymerase chain reaction
What does a restriction enzyme do?
O a) Delivers recombinant DNA to target cells.
O b) Cuts DNA at a particular sequence.
c) Makes synthetic DNA.
O d) Joins together pieces of DNA from different sources.
Chapter 15 Solutions
BIOLOGY >PRINT UPGRADE<
Ch. 15.1 - Prob. 1LOCh. 15.1 - Explain how gel electrophoresis is used to...Ch. 15.1 - Describe how PCR is used to amplify a specific...Ch. 15.1 - Compare the possible differences between a...Ch. 15.1 - Prob. 1CCh. 15.1 - Different forms of a protein are produced in the...Ch. 15.1 - What advantages does the PCR method have over gene...Ch. 15.2 - Describe the features of a typical CRISPR locus in...Ch. 15.2 - Explain the function of CRISPR in bacterial cells.Ch. 15.2 - Compare CRISPR-based endonucleases with...
Ch. 15.2 - Prob. 8LOCh. 15.2 - Prob. 1CCh. 15.2 - Prob. 2CCh. 15.2 - Prob. 3CCh. 15.3 - Prob. 9LOCh. 15.3 - Prob. 10LOCh. 15.3 - Discuss how qPCR, DNA microarrays (DNA chips), and...Ch. 15.3 - Explain how you would compare the expression of a...Ch. 15.3 - Prob. 2CCh. 15.4 - Describe how genome-wide association studies have...Ch. 15.4 - Explain how targeted gene silencing and knockout...Ch. 15.4 - Prob. 1CCh. 15.5 - Describe at least one important application of DNA...Ch. 15.5 - Prob. 1CCh. 15.5 - What are short tandem repeats (STRs), and why are...Ch. 15.5 - Why do gene targeting and mutagenesis screening in...Ch. 15.6 - Prob. 15LOCh. 15.6 - Prob. 16LOCh. 15.6 - Prob. 1CCh. 15.6 - Prob. 2CCh. 15.7 - Describe at least two safety issue associated with...Ch. 15.7 - What are some of the environment concerns...Ch. 15 - A plasmid (a) can be used as a DNA vector (b) is a...Ch. 15 - DNA molecules with complementary sticky ends...Ch. 15 - Prob. 3TYUCh. 15 - Which technique rapidly replicated specific DNA...Ch. 15 - Prob. 5TYUCh. 15 - A cDNA clone contains (a) introns (b) exons (c)...Ch. 15 - Prob. 7TYUCh. 15 - Gel electrophoresis separates nucleic acids on the...Ch. 15 - A CRISPR locus in a bacterium contains (a) short...Ch. 15 - DNA molecular with complementary sticky ends...Ch. 15 - These highly polymorphic molecular markers are...Ch. 15 - Prob. 12TYUCh. 15 - Prob. 13TYUCh. 15 - Prob. 14TYUCh. 15 - EVOLUTION LINK DNA technology, such as the...Ch. 15 - SCIENCE, TECHNOLOGY, AND SOCIETY What are some...
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- These highly polymorphic molecular markers are useful in DNA fingerprinting: (a) plasmid vectors (b) cloned DNA sequences (c) palindromic DNA sequences (d) short tandem repeats (e) complementary DNAsarrow_forwardWhat do genetic engineers use to create the “sticky ends” needed to splice two fragments of DNA together? a.) an amino acid sequence b.) DNA ligase c.) restriction enzymes d.) mRNAarrow_forwardIn Cohen-Boyer’s recombinant DNA procedure, ___i___ must be used for both the bacterial DNA and the amphibian DNA ___ii___ a) the same restriction enzyme; so that the restriction sites are identical in the DNA of each species b) different restriction enzymes; So that the genes outside the restriction site are maintained c) different restriction enzymes; to ensure that the newly introduced genes are maintained in the bacterial DNA d) the same restriction enzyme; to ensure that the newly formed DNA can replicatearrow_forward
- Bioinformatics includes all of the following except (A) using computer programs to align DNA sequences. (B) using DNA technology to combine DNA from two different sources in a test tube. (C) developing computer-based tools for genome analysis. (D) using mathematical tools to make sense of biological systems.arrow_forwardArrange the following steps in the sequence they would happen in a DNA cloning experiment. a. sealing DNA fragments into vectors with DNA ligase; b. utilizing a probe to detect a clone in the library; c. sequencing the clone's DNA; d. creating a DNA library of clones; e. cutting genomic DNA with restriction enzymes. A. e,a,d,b,c B. a,d,b,c,e C. c,b,e,a,d D. e,d,a,c,barrow_forwardTissue growth factor-β (a) is a DNA probe for recombinant plasmids (b) is a product of DNA technology used in tissue engineering (c) is necessary to make cDNA (d) cannot be synthesized without a heat-resistant DNA polymerase (e) is isolated by the Southern blot techniquearrow_forward
- In PCR , the primers will determine which gene will amplified (copied) . in lab we’re doing qRT- PCR using PAL primers and pair of primers for an RRNA gene what would happen is we setup a PCA and used primers for myostatin - what gene would be amplified (copied) ? A) any gene could be Amplified B) myostatin C) PAL D) no gene would be amplifiedarrow_forwardWhich of the following tools of DNA technology is incorrectlypaired with its use?(A) electrophoresis—separation of DNA fragments(B) DNA ligase—cutting DNA, creating sticky ends of restriction fragments(C) DNA polymerase—polymerase chain reaction to amplifysections of DNA(D) reverse transcriptase—production of cDNA frommRNAarrow_forwardHow are "sticky ends" generated in DNA? A) by gel electrophoresis B) by cutting with restriction enzymes C) by using DNA polymerase D) by adding DNA ligase E) both A and B are correctarrow_forward
- Which of the following is NOT needed to perform a PCR reaction? A) DNA primers B) deoxyguanosine triphosphate C) cytosine triphosphate D) Taq DNA ploymerase E) template DNAarrow_forwardGENETICS The modified "dye terminator method for DNA sequencing represented a major improvement over Sanger's original method because ( among other things) it does NOT require a) a DNA primer b) DNA polymerase c) the use of four separate sequencing reactions for each template d) the use of electrophoresis to separate DNA chains based on size e) the used chemically modified dNTPs f) all of the abovearrow_forwardWhat are the main components needed for electrophoresis? a) A power source b) A gel matrix c) A medium (or "buffer") d) Casting tray e) All of the above For what purpose is DNA electrophoresis used? a) To amplify a DNA for cloning b) To separate noncharged molecules of DNA based on size c) To separate charged molecules of DNA based on size d) To separate charged molecules of DNA based on amount of positive charge e) To produce static electricity by frictionarrow_forward
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