Which of the following is NOT needed to perform a PCR reaction? A) DNA primers B) deoxyguanosine triphosphate cytosine triphosphate D) Taq DNA ploymerase E) template DNA

Q: In pcr experiment, Does electrophoresis show that only DNA products of the desired size are present?…

A: A DNA sequence may be amplified trillions of times via PCR, yielding enough DNA copies for further…

Q: GENETICS The modified "dye terminator method for DNA sequencing represented a major improvement…

A: Answer is f.) all of the above.

Q: Your company has decided to develop a PCR test that will be used to determine Huntington's disease.…

A: The polymerase chain reaction (PCR), is one of the most useful molecular biology techniques used to…

Q: You want to set up 50 µl total volume of a PCR reaction. You have a microfuge tube of Taq polymerase…

A:

Q: In a PCR reaction to amply a particular gene, 120 copy of the gene were initially used as DNA…

A: Here, option A is the right answer. You may use the following formula to calculate the number of…

Q: What is real-time PCR?

A: Besides tools, there a several techniques which are used in recombinant DNA technology. These…

Q: Would you be able to replace the Taq polymerase with human polymerase in the PCR reaction? Explain…

A: PCR stands for the Polymerase chain reaction.This technique is developed by the Kary Mullis in…

Q: There are four possible observations that suggest an error occurred in a PCR reaction: a) Sequence…

A: PCR reaction is a method used to amplify a small sample into millions of small ones which help the…

Q: Why is PCR beneficial?

A: PCR is a very useful tool and has many applications in various fields such as medical, forensic,…

Q: What are the advantages of using a recombinant DNA product from a human source instead of a product…

A: GENETIC ENGINEERING:- It is a modification in genes that is done using biotechnology ( Recombinant…

Q: Where does a restriction endonuclease cuts DNA?a) Ligation siteb) Recognition sequencec) Orid)…

A: Restriction endonucleases are enzymes that cut the DNA strand at a specific sequence. They are…

Q: In a sequencing reaction, the dATP was left out of the tube.What would be the result of this…

A: Polymerase chain reaction (PCR) is a technique broadly used to quickly make millions to billions of…

Q: Which of the following process generates a new copy of the transposable element at a new location of…

A: Transposition is a DNA recombination reaction that results in the translocation of a discrete DNA…

Q: Which is used for cloning eukaryotic genes but not prokaryotic genes?a) Restriction enzymesb) DNA…

A: Restriction enzymes are the enzymes that cleave the deoxyribonucleic acid (DNA) at specific sites…

Q: Why didn't Rosalind Franklin get her due recognition for her work with DNA? A)All of the options…

A: Rosalind Franklin discovered the density of DNA and a clearer X-ray pattern of DNA molecules.

Q: What are the disadvantages of doing DNA sequencing?

A: DNA sequencing is a process of identifying the order of nucleic acid bases i.e. adenine, guanine,…

Q: A DNA library is aa) A DNA fragment inserted into a vector.b) A general collection of all genes…

A: The cDNA is called the complementary DNA and is synthesized from the single-stranded RNA or mRNA or…

Q: Amplified target regions of four different samples were separated using gel electrophoresis.  DNA…

A: Gel electrophoresis is a lab technique for separating DNA, RNA, and protein mixture based on their…

Q: Which of the following is helpful in distinguishing DNA of one individual from another?a) PCRb)…

A: DNA is found in almost every living cell. However, whether that cell possesses a special…

Q: Why is it necessary to use a special DNA polymerase(Taq polymerase) in PCR?

A: PCR (Polymerase Chain Reaction) is a technique that is used to amplify specific DNA sequences. The…

Q: HI Molecule 2 Molecule 3 Legend: Thick black line - RNA. Thin black line - DNA. Thin dashed line -…

A: The polymerase chain reaction was given in 1985 by " Kary Mullis". It is a rapid in-vitro molecular…

Q: What are the criteria for “good” primers in a PCR reaction?

A: Polymerase chain reaction (PCR) is the method that is widely used to make billions of copies of a…

Q: What is Recombinant DNA technology ? Who created this ?

A: DNA is the genetic material in all the organisms except certain viruses. It is a polymer of…

Q: In your PCR reaction, you were able to use the same primer set, even though you were likely…

A: PCR is a molecular biology tool that is useful in a variety of ways. It is used in forensic…

Q: Why is the use of a heat-stable DNA polymerase important to the success of PCR?

A: The polymerase chain reaction (PCR) is a method used to amplify the deoxyribonucleic acid (DNA) of…

Q: Most PCR reactions do not use the more expensive types of DNA polymerase, which have DNA…

A: DNA polymerases inside the cell, especially the polymerases I, II, and III, have proof reading…

Q: How is DNA amplified in a polymerase chain reaction(PCR) procedure?

A: Step 1 Polymerase chain reaction (PCR) sometimes also called molecular photocopying is a technique…

Q: What is the nature of the primers used in RT-PCR tests? (i.e. are they DNA primers or RNA primers?)

A: In RT-PCR, the RNA sample obtained from a virus is converted into DNA, and the DNA is amplified and…

Q: What is the purpose of adding a primer to a PCR reaction? Is this primer made up of DNA or RNA…

A: The polymerase chain reaction is a method that is widely used to rapidly make millions of copies of…

Q: What is the major difference in the functions performed by the conventional PCR and real time PCR?…

A: PCR REAL TIME PCR A technique to amplify a segment of DNA , generating millions of copies of a DNA…

Q: 8) Ramachandran plot (how to read it) 19 )Rotations around bonds in a peptide sequence 20) DNA and…

A: The Ramachandran plot is a tool for visualising the allowed and disallowed protein backbone…

Q: A DNA polymerase enzyme inherently incorporates an incorrect nucleotide at a low but measurable…

A: The germ "mutation" is used to define any condition where there is any kind of alteration in the…

Q: During PCR amplification in preparation for DNA sequencing, why were there different colors at the…

A: Polymerase chain reaction is a process by which millions of copies of a specific DNA segment are…

Genetic Recombination

Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.

Microbial Genetics

Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.