Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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Transcribed Image Text:Which of the following is NOT needed to perform a PCR reaction?
A) DNA primers
B) deoxyguanosine triphosphate
C) cytosine triphosphate
D) Taq DNA ploymerase
E) template DNA
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- What is the role of the liquid detergent used in the experiment? a) It allows the DNA to unwind. b) It aids filtration. c) It helps break down the cell membrane. d) It helps keep the DNA soluble in solution.arrow_forwardWhy is it important to include a selective marker gene on a plasmid? a) So that you can select cells containing the plasmid after transformation. b) So that you can select where the plasmid inserts into the genome. Oc) So that you can select the recombinant gene you want to add to the plasmid. d) So that you can select when the recombinant gene on your plasmid is expressed.arrow_forwardIf you use the pUC18 vector to clone in the MCS region, predict the following: a) Do bacteria that are blue in color have a cloned insert? b)Do bacteria that are white in color have a cloned insert? c) If you were to grow these cells on Chloramphenicol (an antibiotic), would the bacteria with the pUC plasmid grow? Why or Why not?arrow_forward
- Which of the following does NOT require a primer? A) PCR-based viral test B) cloning a human gene from mRNA C) subcloning a bacterial gene from one plasmid to another D) DNA fingerprinting for paternity testing E) DNA sequencingarrow_forwardA recombinant plasmid can be used to a.) prevent nondisjunction at meiosis. b.) double the number of chromosomes in a plant cell. c.) cut DNA into restriction fragments. d.) transform a bacteriumarrow_forwardYou set aside some of your purified PCR product to run on a gel. Name two things we learn by running our DNA on an agarose gel electrophoresis?arrow_forward
- You want to propagate large amounts of a DNA fragment. To do this, use a plasmid vector. a) How should this plasmid vector be constructed? b) Also describe the function of the components.arrow_forwardYou isolate bacterial DNA from an unknown species, and use a PCR primer that amplifies DNA only from the bacterial genus Staphylococcus. After PCR and gel electrophoresis, you do not see any bands of DNA on your gel.What does this mean?arrow_forwardTEIIF Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a a) comes to the complex with the polymerase b b) loosens non-specific interactions with the DNA c) both a and b d) none of the abovearrow_forward
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