Which of the following is NOT needed to perform a PCR reaction? A) DNA primers B) deoxyguanosine triphosphate cytosine triphosphate D) Taq DNA ploymerase E) template DNA

Q: Match the reagent with technique. Answer all for upvote Recombinant DNA technology a.)…

A: Recombinant DNA technology is a technology in which a set of techniques are used which enables the…

Q: Which of the following enzyme is used in PCR?a) Taq DNA polymeraseb) HRPc) EcoRId) EcoRII

A: Polymerase chain reaction (PCR) is a laboratory technique, which is used to make multiple copies of…

Q: What are the main components needed for electrophoresis? a) A power source b) A gel matrix c) A…

A: 1.  Option e is the correct answer All of the above are required for electrophoresis.

Q: PCR has many useful applications. However, an incorrect application of PCR is: a) Amplification of…

A: DNA or Deoxyribonucleic acid is a macromolecule which is responsible for the transfer of hereditary…

Q: DEFINE THE FOLLOWING: 1) restriction enzyme 2) plasmid 3) recombinant DNA

A: Ans 1) Restriction enzymes are enzymes that are isolated from bacteria that can cleave…

Q: ou followed all the steps correctly with a kit you bought last week. However, NO purified DNA came…

A: Plasmids exist naturally in bacterial cells . In some cases bacteria need to pick up plasmid from…

Q: Primer design for SLIC cloning and the protocol

A: Hi! Thank you for the questions. As you have posted multiple questions, I will be answering the…

Q: 33 - The correct procedures for gene cloning are given as a) PCR/transformation/ligation/restriction…

A: Gene cloning is the method of production of numerous copies of a gene fragment.

Q: There are four possible observations that suggest an error occurred in a PCR reaction: a) Sequence…

A: PCR reaction is a method used to amplify a small sample into millions of small ones which help the…

Q: What are the advantages of using a recombinant DNA product from a human source instead of a product…

A: GENETIC ENGINEERING:- It is a modification in genes that is done using biotechnology ( Recombinant…

Q: To make the recombinant plasmid permeable to DNA molecules, which of the chemicals is added?a)…

A: The correct option is  CaCl2 (b) CaCl2 is the chemical which is added to prevent degradation of the…

Q: What are the disadvantages of doing DNA sequencing?

A: DNA sequencing is a process of identifying the order of nucleic acid bases i.e. adenine, guanine,…

Q: Assuming you started with only one copy of a DNA sequence, how many copies should be present after…

A: Polymerase chain reaction (PCR) is a method applied in molecular biology for DNA amplification. It…

Q: Which of the following is helpful in distinguishing DNA of one individual from another?a) PCRb)…

A: DNA is found in almost every living cell. However, whether that cell possesses a special…

Q: Why is it important to include a selective marker gene on a plasmid? O a) So that you can select…

A: Bacteria are common source of plasmids. For the sole purpose of Cloning experiments, new plasmids…

Q: How are "sticky ends" generated in DNA? A) by gel electrophoresis O B) by cutting with restriction…

A: Sticky ends or the conhesive ends are the overhangs created in a Dna strand, generally using an…

Q: new dNTPs can be added at the start of replication, this is done by an enzyme called      RNA…

A: DNA is made up of deoxyribonucleotides. DNA exist in two strands and both the strands are linked by…

Q: From where do we get primers for sequencing DNA? A) they are synthesized by reverse transcriptase…

A: Primer is a short piece of ribonucleic acid sequence that provides a starting point for DNA…

Q: What will happen to the concentration and the A260/280 ratio of the DNA sample in the following…

A: Biotechnology is the use of our understanding of biological processes to develop useful applications…

Q: Use the figure of a plasmid below to answer the following questions. 43) Which enzyme was used to…

A: Bacteria contain Chromosomal DNA as a genetic material but apart from that it also possess rounded…

Q: y is DNA ligase so important in recombinant DNA technology? a.) It causes DNA to make multiple…

A: Recombination is the process of breaking and recombining fragments of DNA to create new allele…

Q: What is Recombinant DNA technology ? Who created this ?

A: DNA is the genetic material in all the organisms except certain viruses. It is a polymer of…

Q: E) double stranded DNA separates into single stranded DNA

A: The correct option is E . Double stranded DNA separates into Single stranded DNA .

Q: In your PCR reaction, you were able to use the same primer set, even though you were likely…

A: PCR is a molecular biology tool that is useful in a variety of ways. It is used in forensic…

Q: Based on the following image: A) Identify the largest DNA fragment in sample 5. Explain your…

A: In the given question, an agarose gel is shown which is having bands of DNA in different lanes. DNA…

Q: For what purpose is DNA fingerprinting used A) to sequence DNA from bacteria B) to separate DNA…

A: DNA fingerprinting:                 A method of isolating and identifying variable elements within…

Q: 2a) With the aid of diagrams, describe the critical role of restriction enzymes in genetic…

A: Genetic Engineering is combining DNA from two or more different organisms to create recombinant DNA.

Q: 8) Capillary electrophoresis involves a) Fragmentation of the DNA strands b) High resolution…

A: Capillary electrophoresis is an experimental methodology that provides an applied voltage to…

Q: 1) Follow the steps involved in DNA or gene cloning

A: DNA( deoxyribonucleic acid) is the double-stranded molecule that is the genetic material in most…

Q: How is DNA amplified in a polymerase chain reaction(PCR) procedure?

A: Step 1 Polymerase chain reaction (PCR) sometimes also called molecular photocopying is a technique…

Q: The first publications describing the successful production and intracellular replication of…

A: The production of first recombinant DNA takes place in 1970 with the help of the restriction…

Q: What is the nature of the primers used in RT-PCR tests? (i.e. are they DNA primers or RNA primers?)

A: In RT-PCR, the RNA sample obtained from a virus is converted into DNA, and the DNA is amplified and…

Q: In sequencing methods, the role of the electrophoresis is a)To stop the replication at that base…

A: Electrophoresis is a technique used in DNA sequencing methods to separate DNA fragments based on…

Q: The percentage of the agarose gel is important because a) it influences the rate of migration of…

A: 1. Agarose gel is widely used for the separation of DNA and RNA molecules and their visualization.…

Q: 8) Ramachandran plot (how to read it) 19 )Rotations around bonds in a peptide sequence 20) DNA and…

A: The Ramachandran plot is a tool for visualising the allowed and disallowed protein backbone…

Q: Which of the following enzyme is used in PCR? a) EcoRII b) EcoRI c) Tag DNA polymerase d) HRP

A: PCR (Polymerase Chain Reaction) is a widely used technique in molecular biology that amplifies a…

Q: High Frequency Recombination results in which of the following? O 1) Plasmid DNA incorporated into…

A: The bacterial DNA is arranged as a circular chromosome. The bacterial genome has a single origin of…

Q: You have ligated a foreign DNA fragment into the EcoRI site in multiple cloning site of pUCI18. You…

A: Genetic engineering is a branch of biotechnology that involves modifying DNA to create new traits or…

Q: A [ Select ] * strain is one in which a plasmid has integrated into the chromosome via homologous…

A: Recombination in bacteria can be defined as genetic recombination  in which  DNA transferred from…

Q: An 9 kb circular plasmid is cut with the EcoRI restriction enzyme, and the reaction products are run…

A: Restriction enzymes are special enzymes that are known as nucleotide scissors as their role in cells…

Genetic Recombination

Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.

Microbial Genetics

Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.