Biochemistry: The Molecular Basis of Life
Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 14, Problem 5Q
Summary Introduction

To review:

The biosynthetic pathway for creatine.

Introduction:

Creatine is an organicacid. It primarily functionsin the recycling of adenosine triphosphate (ATP) in the muscles and the brain. On the reaction of ATP and creatin, ehosphor creatine is formed. It stores high-energy phosphate for some time. Phosphocreatine breaks when energy demand is high.

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The hydrolysis of pyrophosphate to orthophosphate drives biosynthetic reactions such as DNA synthesis. In Escherichia coli, a pyrophosphatase catalyzes this hydrolytic reaction. The pyrophosphatase has a mass of 120 kDa and consists of six identical subunits. A unit of activity for this enzyme, U, is the amount of enzyme that hydrolyzes 10 umol of pyrophosphate in 15 minutes. The purified enzyme has a Vnax of 2800 U per milligram of enzyme. When (S] >> KM, how many micromoles of substrate can 1 mg of enzyme hydrolyze per second? Vnas umol-s. mg-! max If each enzyme subunit has one active site, how many micromoles of active sites, or (E]r, are there in 1 mg of enzyme? (Er= umol · mg-!
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