HUMAN ANATOMY
6th Edition
ISBN: 9781260210262
Author: SALADIN
Publisher: RENT MCG
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Chapter 14, Problem 14.3.4AYLO
The differences between ipsilateral, contralateral, and intersegmental reflex arcs, and some reflexes that would employ each type
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 14 Solutions
HUMAN ANATOMY
Ch. 14.1 - Prob. 1AWYKCh. 14.1 - Prob. 1BYGOCh. 14.1 - Prob. 2BYGOCh. 14.1 - Prob. 3BYGOCh. 14.1 - Prob. 4BYGOCh. 14.1 - Identify each of the following spinal tracts with...Ch. 14.2 - How does the structure of a nerve compare to that...Ch. 14.2 - Prob. 6BYGOCh. 14.2 - Prob. 7BYGOCh. 14.2 - List the five plexuses of spinal nerves and state...
Ch. 14.2 - State which plexus gives rise to each of the...Ch. 14.3 - Prob. 1AWYKCh. 14.3 - Prob. 10BYGOCh. 14.3 - Prob. 11BYGOCh. 14.3 - Describe a situation in which each of the...Ch. 14.4 - Prob. 1AWYKCh. 14.4 - Prob. 13BYGOCh. 14.4 - Prob. 14BYGOCh. 14.4 - Prob. 15BYGOCh. 14 - Four functions of the spinal cord and their...Ch. 14 - Prob. 14.1.2AYLOCh. 14 - Prob. 14.1.3AYLOCh. 14 - Prob. 14.1.4AYLOCh. 14 - Prob. 14.1.5AYLOCh. 14 - Prob. 14.1.6AYLOCh. 14 - Prob. 14.1.7AYLOCh. 14 - Prob. 14.1.8AYLOCh. 14 - The funiculi and tracts of spinal white matterCh. 14 - Prob. 14.1.10AYLOCh. 14 - Prob. 14.1.11AYLOCh. 14 - Prob. 14.1.12AYLOCh. 14 - Prob. 14.1.13AYLOCh. 14 - Prob. 14.2.1AYLOCh. 14 - The differences between afferent and efferent...Ch. 14 - Prob. 14.2.3AYLOCh. 14 - Prob. 14.2.4AYLOCh. 14 - The number of spinal nerves and the system for...Ch. 14 - The structure of the proximal portion of a spinal...Ch. 14 - Prob. 14.2.7AYLOCh. 14 - The five plexuses of spinal nerves-their names,...Ch. 14 - Prob. 14.3.1AYLOCh. 14 - The components of a reflex arc and the path...Ch. 14 - Prob. 14.3.3AYLOCh. 14 - The differences between ipsilateral,...Ch. 14 - Prob. 14.3.5AYLOCh. 14 - Prob. 14.4.1AYLOCh. 14 - Prob. 14.4.2AYLOCh. 14 - Prob. 14.4.3AYLOCh. 14 - Below L2, the vertebral canal is occupied by a...Ch. 14 - The brachial plexus gives rise to all of the...Ch. 14 - Prob. 3TYRCh. 14 - Prob. 4TYRCh. 14 - Prob. 5TYRCh. 14 - Prob. 6TYRCh. 14 - In the spinal cord, the neurosomas of the lower...Ch. 14 - Prob. 8TYRCh. 14 - Prob. 9TYRCh. 14 - Prob. 10TYRCh. 14 - Prob. 11TYRCh. 14 - Prob. 12TYRCh. 14 - Prob. 13TYRCh. 14 - Motor innervation of the leg proper comes...Ch. 14 - Prob. 15TYRCh. 14 - The _______ nerves arise from the servical plexus...Ch. 14 - The crossing of a nerve fiber actract from the...Ch. 14 - Prob. 18TYRCh. 14 - Prob. 19TYRCh. 14 - Prob. 20TYRCh. 14 - Prob. 1BYMVCh. 14 - Prob. 2BYMVCh. 14 - Prob. 3BYMVCh. 14 - Prob. 4BYMVCh. 14 - State a meaning of each word element and give a...Ch. 14 - Prob. 6BYMVCh. 14 - Prob. 7BYMVCh. 14 - Prob. 8BYMVCh. 14 - Prob. 9BYMVCh. 14 - Prob. 10BYMVCh. 14 - Prob. 1WWWTSCh. 14 - Prob. 2WWWTSCh. 14 - Prob. 3WWWTSCh. 14 - Briefly explain why each of the following...Ch. 14 - Prob. 5WWWTSCh. 14 - Prob. 6WWWTSCh. 14 - Prob. 7WWWTSCh. 14 - Briefly explain why each of the following...Ch. 14 - Prob. 9WWWTSCh. 14 - Briefly explain why each of the following...Ch. 14 - Prob. 1TYCCh. 14 - Prob. 2TYCCh. 14 - Anthony gets into a fight between rival gangs. As...Ch. 14 - When a patient needs a tendon graft, surgeons...
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