Pyruvate isolate the liver cells in the presence of sufficient malonate to completely block succinate dehydrogenate. The isolated isocitrate contain label in both carbon 2 and carbon 5, the result should be explained. Concept Introduction: The enzyme responsible for the citrate-isocitrate transformation is aconitate hydratase or aconitase. It binds to the intermediate cis-aconitate and prevent its release into the cell solution. It requires Fe 2+ ion for its activity and the participation of a sulfhydryl (-SH) group of cysteine or glutothione is essential. Model study with deuterium labelled substrate indicated trans-addition of H and OH groups to the C=C bond of cis-aconitate. The popularly known ferrous wheel mechanism for the transformation was derived from X-ray diffraction study of Fe 2+ complexes with these tricarboxylic acids. Pictorial representation: The generation of twice labelled carbon atom at isocitrate starting from mono labelled methyl pyruvate molecule is shown as-

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Answer 1

Question

Chapter 13, Problem 4P

Interpretation Introduction

Interpretation:

Pyruvate isolate the liver cells in the presence of sufficient malonate to completely block succinate dehydrogenate. The isolated isocitrate contain label in both carbon 2 and carbon 5, the result should be explained.

Concept Introduction:

The enzyme responsible for the citrate-isocitrate transformation is aconitate hydratase or aconitase. It binds to the intermediate cis-aconitate and prevent its release into the cell solution. It requires Fe2+ ion for its activity and the participation of a sulfhydryl (-SH) group of cysteine or glutothione is essential. Model study with deuterium labelled substrate indicated trans-addition of H and OH groups to the C=C bond of cis-aconitate. The popularly known ferrous wheel mechanism for the transformation was derived from X-ray diffraction study of Fe2+ complexes with these tricarboxylic acids.

Pictorial representation:

The generation of twice labelled carbon atom at isocitrate starting from mono labelled methyl pyruvate molecule is shown as-

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Answer 2

Question

Chapter 13, Problem 4P

Interpretation Introduction

Interpretation:

Pyruvate isolate the liver cells in the presence of sufficient malonate to completely block succinate dehydrogenate. The isolated isocitrate contain label in both carbon 2 and carbon 5, the result should be explained.

Concept Introduction:

The enzyme responsible for the citrate-isocitrate transformation is aconitate hydratase or aconitase. It binds to the intermediate cis-aconitate and prevent its release into the cell solution. It requires Fe2+ ion for its activity and the participation of a sulfhydryl (-SH) group of cysteine or glutothione is essential. Model study with deuterium labelled substrate indicated trans-addition of H and OH groups to the C=C bond of cis-aconitate. The popularly known ferrous wheel mechanism for the transformation was derived from X-ray diffraction study of Fe2+ complexes with these tricarboxylic acids.

Pictorial representation:

The generation of twice labelled carbon atom at isocitrate starting from mono labelled methyl pyruvate molecule is shown as-

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Answer 3

Question

Chapter 13, Problem 4P

Interpretation Introduction

Interpretation:

Pyruvate isolate the liver cells in the presence of sufficient malonate to completely block succinate dehydrogenate. The isolated isocitrate contain label in both carbon 2 and carbon 5, the result should be explained.

Concept Introduction:

The enzyme responsible for the citrate-isocitrate transformation is aconitate hydratase or aconitase. It binds to the intermediate cis-aconitate and prevent its release into the cell solution. It requires Fe2+ ion for its activity and the participation of a sulfhydryl (-SH) group of cysteine or glutothione is essential. Model study with deuterium labelled substrate indicated trans-addition of H and OH groups to the C=C bond of cis-aconitate. The popularly known ferrous wheel mechanism for the transformation was derived from X-ray diffraction study of Fe2+ complexes with these tricarboxylic acids.

Pictorial representation:

The generation of twice labelled carbon atom at isocitrate starting from mono labelled methyl pyruvate molecule is shown as-

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Answer 4

Question

Chapter 13, Problem 4P

Interpretation Introduction

Interpretation:

Pyruvate isolate the liver cells in the presence of sufficient malonate to completely block succinate dehydrogenate. The isolated isocitrate contain label in both carbon 2 and carbon 5, the result should be explained.

Concept Introduction:

The enzyme responsible for the citrate-isocitrate transformation is aconitate hydratase or aconitase. It binds to the intermediate cis-aconitate and prevent its release into the cell solution. It requires Fe2+ ion for its activity and the participation of a sulfhydryl (-SH) group of cysteine or glutothione is essential. Model study with deuterium labelled substrate indicated trans-addition of H and OH groups to the C=C bond of cis-aconitate. The popularly known ferrous wheel mechanism for the transformation was derived from X-ray diffraction study of Fe2+ complexes with these tricarboxylic acids.

Pictorial representation:

The generation of twice labelled carbon atom at isocitrate starting from mono labelled methyl pyruvate molecule is shown as-

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Answer 5

Chapter 13, Problem 4P

Interpretation Introduction

Interpretation:

Pyruvate isolate the liver cells in the presence of sufficient malonate to completely block succinate dehydrogenate. The isolated isocitrate contain label in both carbon 2 and carbon 5, the result should be explained.

Concept Introduction:

The enzyme responsible for the citrate-isocitrate transformation is aconitate hydratase or aconitase. It binds to the intermediate cis-aconitate and prevent its release into the cell solution. It requires Fe2+ ion for its activity and the participation of a sulfhydryl (-SH) group of cysteine or glutothione is essential. Model study with deuterium labelled substrate indicated trans-addition of H and OH groups to the C=C bond of cis-aconitate. The popularly known ferrous wheel mechanism for the transformation was derived from X-ray diffraction study of Fe2+ complexes with these tricarboxylic acids.

Pictorial representation:

The generation of twice labelled carbon atom at isocitrate starting from mono labelled methyl pyruvate molecule is shown as-

Answer 6

Chapter 13, Problem 4P

Interpretation Introduction

Interpretation:

Pyruvate isolate the liver cells in the presence of sufficient malonate to completely block succinate dehydrogenate. The isolated isocitrate contain label in both carbon 2 and carbon 5, the result should be explained.

Concept Introduction:

The enzyme responsible for the citrate-isocitrate transformation is aconitate hydratase or aconitase. It binds to the intermediate cis-aconitate and prevent its release into the cell solution. It requires Fe2+ ion for its activity and the participation of a sulfhydryl (-SH) group of cysteine or glutothione is essential. Model study with deuterium labelled substrate indicated trans-addition of H and OH groups to the C=C bond of cis-aconitate. The popularly known ferrous wheel mechanism for the transformation was derived from X-ray diffraction study of Fe2+ complexes with these tricarboxylic acids.

Pictorial representation:

The generation of twice labelled carbon atom at isocitrate starting from mono labelled methyl pyruvate molecule is shown as-

Answer 7

Chapter 13, Problem 4P

Interpretation Introduction

Interpretation:

Pyruvate isolate the liver cells in the presence of sufficient malonate to completely block succinate dehydrogenate. The isolated isocitrate contain label in both carbon 2 and carbon 5, the result should be explained.

Concept Introduction:

The enzyme responsible for the citrate-isocitrate transformation is aconitate hydratase or aconitase. It binds to the intermediate cis-aconitate and prevent its release into the cell solution. It requires Fe2+ ion for its activity and the participation of a sulfhydryl (-SH) group of cysteine or glutothione is essential. Model study with deuterium labelled substrate indicated trans-addition of H and OH groups to the C=C bond of cis-aconitate. The popularly known ferrous wheel mechanism for the transformation was derived from X-ray diffraction study of Fe2+ complexes with these tricarboxylic acids.

Pictorial representation:

The generation of twice labelled carbon atom at isocitrate starting from mono labelled methyl pyruvate molecule is shown as-

Answer 8

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Answer 11

Ch. 13 - Prob. 1P Ch. 13 - Prob. 2P Ch. 13 - Suppose that aconitase did not bind its substrate...Ch. 13 - Prob. 4P Ch. 13 - Prob. 5P Ch. 13 - Prob. 6P Ch. 13 - Prob. 7P Ch. 13 - Prob. 8P Ch. 13 - Prob. 9P Ch. 13 - Prob. 10P

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