Concept explainers
(a)
Interpretation:
Theabsorbance of the solution for the percent transmittance for half of
Concept introduction:
The absorbance of the solution is the ability of the solution to absorb the monochromatic light passing through it. The absorbance of the solution is defined as the ratio of the intensity of light incident on the solution to the intensity of light absorbed by the solution.
The relation between the absorbance and transmittance of a solution is given by Beer’s law.
(b)
Interpretation:
Theabsorbance of the solution for the percent transmittance for half of
Concept introduction:
The absorbance of the solution is the ability of the solution to absorb the monochromatic light passing through it. The absorbance of the solution is defined as the ratio of the intensity of light incident on the solution to the intensity of light absorbed by the solution.
The relation between the absorbance and transmittance of a solution is given by Beer’s law.
(c)
Interpretation:
Theabsorbance of the solution for the percent transmittance of half of
Concept introduction:
The absorbance of the solution is the ability of the solution to absorb the monochromatic light passing through it. The absorbance of the solution is defined as the ratio of the intensity of light incident on the solution to the intensity of light absorbed by the solution.
The relation between the absorbance and transmittance of a solution is given by Beer’s law.
(d)
Interpretation:
Theabsorbance of the solution for the percent transmittance for half of
Concept introduction:
The absorbance of the solution is the ability of the solution to absorb the monochromatic light passing through it. The absorbance of the solution is defined as the ratio of the intensity of light incident on the solution to the intensity of light absorbed by the solution.
The relation between the absorbance and transmittance of a solution is given by Beer’s law.
(e)
Interpretation:
Theabsorbance of the solution for the percent transmittance for half of
Concept introduction:
The absorbance of the solution is the ability of the solution to absorb the monochromatic light passing through it. The absorbance of the solution is defined as the ratio of the intensity of light incident on the solution to the intensity of light absorbed by the solution.
The relation between the absorbance and transmittance of a solution is given by Beer’s law.
(f)
Interpretation:
Theabsorbance of the solution for the percent transmittance of
Concept introduction:
The absorbance of the solution is the ability of the solution to absorb the monochromatic light passing through it. The absorbance of the solution is defined as the ratio of the intensity of light incident on the solution to the intensity of light absorbed by the solution.
The relation between the absorbance and transmittance of a solution is given by Beer’s law.
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Chapter 13 Solutions
Principles of Instrumental Analysis
- A student prepared several solutions of an unknown molecule, each at a different concentration. The absorbance of each solution was measured at 630 nm with a path length of 0.760 cm. The student then plotted the absorbance of each sample vs. its concentration (in uM), and found a best-fit line of y = 0.00626x + 0.00732. Calculate the molar absorptivity of the unknown molecule (in M-1 cm-1).arrow_forwardShow COMPLETE solutions. Round-off FINAL answer to the 4th decimal The absorption coefficient of Sample Z at 340 nm is 600 Mol-1cm-1. What absorbance will be observed when light at 340 nm passes through a 1 cm cuvette containing 0.001M Sample zk A solution put in a 1.5 cm container transmits 55% incident light. Calculate the concentration of the solution, given that ɛ = 6x10-3 Mol-1cm-1. A solution shows a transmittance of 39.72%, when taken in a container measuring 3.5 cm thickness. Calculate its concentration, if the molar absorption coefficient is 3x10-2 Mol-1cm- 1.arrow_forwardBriefly explain why it is NOT accurate to calculate the concentration of a sample whose absorbance is 0.185 using the equation y=0.0022x+0.0005 r^2=0.999arrow_forward
- How does pipetting laboratory techniques affect absorbance values when using UV-Vis Spectrophotometry?arrow_forwardGiven the following sample concentrations and corresponding absorbances, construct a standard curve of absorbance vs. concentration and determine the molar absorptivity constant. Sample Concentration 15 μΜ 25 μΜ 40 μΜ Absorbance 0.19 0.32 0.49 55 μΜ 0.70arrow_forwardDraw a plot having X-axis as concentration (mol/L) and Y-axis as absorbance at λ max (525 nm). Determine the concentration of the unknown sample with absorbance of 2.2.arrow_forward
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- (d) The Beer-Lambert law states that the absorbance (A) of light by a solution in a cuvette is given by: 10 A = In == ECL where lo is the incident light intensity, is the transmitted light intensity, e is the molar absorptivity, c is the concentration of the solution, and L is the path length of the cuvette. (1) Light with a wavelength of 800 nm is passed through a cuvette containing a solution of Coumarin dye. After passing through the cuvette, the light intensity was reduced to 10% of its initial value. What is the absorbance of the solution of Coumarin dye? (ii) The concentration of the Coumarin dye is 0.004 mol dm3, and the cuvette has a path length of 1 cm. What is the molar absorptivity of Coumarin dye at 800 nm, in units of m² mot¹? (IT) The molar absorptivity of Coumarin dye at 400 nm is three times larger than at 800 nm. How would the absorbance of the solution change, when measured with 400 nm light rather than 800 nm light?arrow_forwardA standard curve of absorbance vs. concentration has a linear trendline of y = 0.56x + 0.987. Use this trendline to calculate the concentration of an unknown sample that has an absorbance of 1.245.arrow_forwardWhy is it most accurate to measure absorbances in the range A = 0.3 to 2?arrow_forward
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