Microbiology: An Introduction
11th Edition
ISBN: 9780321733603
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: Benjamin Cummings
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Textbook Question
Chapter 12, Problem 2R
A mixed culture of Escherichia coli and Penicillium chrysogenum is inoculated onto the following culture media. On which medium would you expect each to grow? Why?
- a. 0.5% peptone in tap water
- b. 10% glucose in tap water
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After inoculating and incubating an agar slant from a pure broth culture of a bacterial species such as E. coli, which of the following would indicate an unsuccessful aseptic transfer? (Choose ALL that apply)
a - There is fungal growth in the original broth culture tube.
b- There is too much growth on the agar slant.
c- There are colonies of similar morphology on the slant.
d - There are red, yellow, and white colonies on the slant.
e - There is no growth on the slant.
For each of the following situations,
describe how bacteria on these growth.
media would appear on the plate:
A. On MacConkey agar, a bacterium that
grows on the medium but is negative for
lactose fermentation
B. On EMB agar, a bacterium that grows
on the medium but is positive for lactose
fermentation
C. On mannitol salt agar, a bacterium that
failed to grow on the medium
D. On mannitol salt agar, a bacterium that
grew on the medium and fermented
mannitol
1) based on the difference in appearance between mixed culture streak plates and broth culture plates, what would lead you to believe that you have created a pure culture when you inoculated the broths?
2) when using the loop dilution technique to produce pour plates, did all the plates produce isolated colonies? If your goal was to ultimately create pure cultures of E. coli, M. luteus, and S. morcescens, which plate or which dilution would you use, and why would you use this plate?
3) What are the advantages and disadvantages of streak plating compared with pour plating?
Chapter 12 Solutions
Microbiology: An Introduction
Ch. 12 - Prob. 1RCh. 12 - A mixed culture of Escherichia coli and...Ch. 12 - Prob. 3RCh. 12 - Prob. 4RCh. 12 - Differentiate cellular and plasmodial slime molds....Ch. 12 - Prob. 6RCh. 12 - Prob. 7RCh. 12 - Prob. 8RCh. 12 - Prob. 9RCh. 12 - DRAW IT A generalized life cycle of the liver...
Ch. 12 - How many phyla are represented in the following...Ch. 12 - Prob. 2MCQCh. 12 - Prob. 3MCQCh. 12 - Fleas are the intermediate host for Dioylium...Ch. 12 - Prob. 5MCQCh. 12 - Prob. 6MCQCh. 12 - The definitive host for Plasmodium vivax is a....Ch. 12 - Prob. 8MCQCh. 12 - Prob. 9MCQCh. 12 - Prob. 10MCQCh. 12 - Alexandrium (red tide) has been called a plant...Ch. 12 - The life cycle of the fish tapeworm...Ch. 12 - Trypanosoma brucei gambiense-part (a) in the...Ch. 12 - Prob. 1CAECh. 12 - Prob. 2CAECh. 12 - A teenaged male in California complained of...
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