Prescott's Microbiology
Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 10.4, Problem 1MI

Chapter 10.4, Problem 1MI, Figure 10.6 Electron Movement and Reduction Potentials. Electrons spontaneously move from donors

Figure 10.6 Electron Movement and Reduction Potentials. Electrons spontaneously move from donors higher on the tower (more negative potentials) to acceptors lower on the tower (more positive potentials). That is, the donor is always higher on the tower than the acceptor. For example, NADH will donate electrons to oxygen and form water in the process. Some typical conjugate redox pairs are shown on the left, and their reduction potentials are given in brackets.

Refer to figure 10.6 and determine the E′0 for NAD+/NADH and coenzyme Q/CoQH2. Suggest a plausible E′0 value for FMN.

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Fo-F1 ATPase. The energy for ATP synthesis from ADP and Pi is provided by the downhill transport of protons through the rotary FoF1 ATP synthase (lecture 22).   The enzyme has 3 a-b and 12 ‘c’ subunits. The mitochondrion maintains Df=180 mV (negative inside), pHin = 8, pHout=7, [Pi] = 3 mM and ADP is present as well. How much energy is available (from the proton electrochemical gradient) for ATP synthesis under these conditions (in kJ/mol)?                                                                                                                  What [ATP]/[ADP] ratio will be established at steady-state under these conditions?                                                                                                                                                What would be the [ATP]/[ADP] ratio if the enzyme had only 9 ‘c’ subunits? Remember that full revolution of the crank (gamma subunit) produces 3 ATP.

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