CAMPBEL BIOLOGY:CONCEPTS & CONNECTIONS
10th Edition
ISBN: 9780136538820
Author: Taylor
Publisher: INTER PEAR
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Chapter 10, Problem 9TYK
A cell containing a single chromosome is placed in a medium containing radioactive phosphate so that any new DNA strands formed by
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Shown below is a double stranded DNA molecule.Replicate this DNA and show the products formed from this replication. In your answer, indicate which strands are the new daughter strands (mark with D and in a different color) and which strands are the already existing parent strands (mark with P as shown below). Label the 5’ and 3’ ends of all DNA. HINT: You should have 2 double-stranded DNA molecules after replication.
P: 5’ CATAGGATTCCTACG 3’
P: 3’ GTATCCTAAGGATGC 5’
One strand of a single DNA helix is labeled red while the other strand of the same DNA helix is labeled blue. This double helix DNA is replicated through the process of semi-conservative replication. Note that a completely newly synthesized strand of DNA will be white. After three rounds of DNA replication, how many all-white DNA (both strands are white) will there be? Explain your reasoning.
What proteins are crucial for creating and maintaining DNA replication forks? Choose the best explanation.
Question 2 options:
Helicase creates the replication fork; primase keeps the single strands from closing shut.
Helicase creates the replication fork; single-strand binding proteins keep the single strands from reuniting.
Ligase creates the replication fork; DNA polymerase II keeps the single strands from reuniting.
Helicase creates the replication fork; ligase keeps the single strands from closing shut.
Chapter 10 Solutions
CAMPBEL BIOLOGY:CONCEPTS & CONNECTIONS
Ch. 10 - Check your understanding of the flow of genetic...Ch. 10 - Which of the following correctly ranks the...Ch. 10 - Describe the process of DNA replication: the...Ch. 10 - What is the name of the process that produces RNA...Ch. 10 - Scientists have discovered how to put together a...Ch. 10 - A geneticist found that a particular mutation had...Ch. 10 - Describe the process by which the information in a...Ch. 10 - The nucleotide sequence of a DNA codon is GTA. A...Ch. 10 - A cell containing a single chromosome is placed in...Ch. 10 - The base sequence of the gene coding for a short...
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- There are 6 parts to this question: This is a follow up to the prior question regarding the replication of the DNA strand below. The DNA strand is here for your reference and you do not need to do anything with or to it. TC GATATCGG AGCTATAGCC c) what enzyme separated the parental DNA template strands, d) what bonds were broken? e) what enzyme replicates DNA f) before DNA can be replicated/copied, what must be laid down to allow the enzyme in "e" to replicated the DNA (be specific)? g) our DNA is replicated in many "pieces", what enzyme connects these many "pieces" into one continuous DNA strand that becomes the sister chromatid? h) during what specific phase of the cell cycle does this DNA replication process occur? (This should be a review question from last topics we covered).arrow_forwardA plasmid is a DNA double helix in which the ends of each of the strands of nucleotides are attached to each other, forming a circular DNA molecule. 1) True 2) Falsearrow_forwardBelow is a diagram of DNA replication as currently believed to occur in E. coli. Arrows start from numbers and end at specific points. Answer the questions relating to the locations specified by the numbers (1) Which end (5' or 3') of the molecule is here? (2) Which enzyme is probably functioning here to deal with supercoils in the DNA? (3) Which enzyme is probably functioning here to unwind the DNA?arrow_forward
- DNA polymerase is important in the replication process because it can join together segments of DNA into a single daughter strand. Select one: True Falsearrow_forwardDNA replication is called semi- conservative because of the two new strands of DNA is old DNA from the original DNA molecule and is new DNA. Answers to choose from: 1) Half 2) All 3) Nonearrow_forwardTwo important proteins in DNA replication are (select two): Cdx2 Cdt2 Cdc6 Cdc6 Cdt1 Cdc5arrow_forward
- Match the following descriptions with the enzymes involved in DNA replication. Adds an RNA primer to begin elongation Removes the RNA primer from the beginning of the newly constructed strands Splices lagging strand segments Cleaves the rung of the DNA double helix ladder Description: Answer Answer Answer Answer Enzyme: Helicase DNA Polymerase Primase DNA Ligasearrow_forwardTrue or false: After chromosomal replication, Type I topoisomerase is used to coil up the double-stranded DNA?arrow_forwardThe template (antisense) strands of two complete (double-stranded) DNA molecules have the base sequences shown in the table below. Two replication experiments are done with each molecule: 1. In Experiment #1, samples of each DNA molecule are incubated with radioactive adenine, along with appropriate replication enzymes, ATP, adenine, thymine, and cytosine. Experiment #1 is stopped when each DNA molecule has replicated once. 2. In Experiment #2, all the DNA molecules from #1 are purified, and then incubated with with a similar reaction mixture - except non-radioactive adenine is used this time. Experiment #2 is stopped when each DNA molecule has replicated one more time. Predict the percentage of DNA in each sample that is radioactive after each experiment. Round your answers to the nearest percent. DNA template strand sequence 3'- T C C C T A -5' 3'- G G C C G C -5' % of DNA radioactive after ... Experiment #1 Experiment #2 % % % % X Śarrow_forward
- Assume a deletion occurs in a gene that encodes DNA polymerase I and no functional DNA polymerase I is produced. What will be the most likely consequence of this mutation? The DNA would not exist in a supercoiled state. There would be no RNA primers laid down. The DNA will not be able to unwind to initiate replication. The DNA strands would contain pieces of RNA. There would be no DNA replication on the leading or lagging strands.arrow_forwardTrue or false: The synthesis of a new strand of DNA is initiated using a DNA primer?arrow_forwardDNA replication is semi-conservative, this statement means that Question 6 options: a) the new DNA molecules that are made are not identical to the original DNA molecule. b) the new DNA molecules that are made are only partially DNA since RNA primers are included. c) the new DNA molecules that are made are composed of one strand of the old DNA molecule and one strand of new DNA. d) of the two new DNA molecules made, one is entirely new DNA and one is entirely old new. e) the new DNA molecules that are made have a mixture of old and new DNA in each strand of the DNA duplex, randomly interspersed.arrow_forward
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