BIOCHEMISTRY
9th Edition
ISBN: 2818440090622
Author: BERG
Publisher: MAC HIGHER
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Chapter 1, Problem 5P
Interpretation Introduction
Interpretation:
The entropy change of the system and the surroundings should be determined.
Concept introduction:
According to the second law of
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More ratios. Through the use of nuclear magnetic resonance
spectroscopy, it is possible to determine the ratio between the
protonated and deprotonated forms of buffers. (a) Suppose the ratio
of [ A- ]A I to [HA] is determined to be 0.1 for a buffer with
pKar6.0.pKa = 6.0. What is the pH? (b) For a different buffer,
91974
suppose the ratio of [ A- ]lA J to [HA] is determined to be 0.1 and
the pHpH is 7.0. In this case, what is the pKapKa of the buffer? (c) For
another buffer with pKa=7.5PKa = 7.5 at pH 8.0pH 8.0, what is the
expected ratio of [ A- ][A ] to [HA]? do
Ionization State of Histidine.Each ionizable group of an amino acid can exist in one of two states, charged or neutral. The electric charge on the functional group is determined by the relationship between its pKa and the pH of the solution. This relationship is described by the Henderson-Hasselbalch equation.
1.Histidine has three ionizable functional groups. Write the equilibrium equations for its three ion-izationsand assign the proper pKa for each ionization. Draw the structure of histidine in each ionization state.What is the net charge on the histidine molecule in each ionization state?
2.Which structure drawn in (1) corresponds to theionization state of histidine at pH 1, 4, 8, and12?Note that the ionization state can be approximated by treating each ionizable group independently.
3.What is the net charge of histidine at pH 1, 4, 8, and 12? For each pH, will histidine migrate to-ward the anode (+) or cathode (-) when placed in an electric field?
Sedimenting spheres. What is the dependence of the sedimentation coefficient s of a spherical protein on its mass?How much more rapidly does an 80 -kDa protein sedimentthan does a 40-kDa protein?
Chapter 1 Solutions
BIOCHEMISTRY
Ch. 1 - Prob. 1PCh. 1 - Prob. 2PCh. 1 - Prob. 3PCh. 1 - Prob. 4PCh. 1 - Prob. 5PCh. 1 - Prob. 6PCh. 1 - Prob. 7PCh. 1 - Prob. 8PCh. 1 - Prob. 9PCh. 1 - Prob. 10P
Ch. 1 - Prob. 11PCh. 1 - Prob. 12PCh. 1 - Prob. 13PCh. 1 - Prob. 14PCh. 1 - Prob. 15PCh. 1 - Prob. 16PCh. 1 - Prob. 17PCh. 1 - Prob. 18PCh. 1 - Prob. 19PCh. 1 - Prob. 20PCh. 1 - Prob. 21PCh. 1 - Prob. 22PCh. 1 - Prob. 23PCh. 1 - Prob. 24PCh. 1 - Prob. 25PCh. 1 - Prob. 26PCh. 1 - Prob. 27PCh. 1 - Prob. 28PCh. 1 - Prob. 29PCh. 1 - Prob. 30PCh. 1 - Prob. 31PCh. 1 - Prob. 32PCh. 1 - Prob. 33PCh. 1 - Prob. 34PCh. 1 - Prob. 35PCh. 1 - Prob. 36P
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