You were working with mouse mitochondrial proteins, and as a routine, you have isolated muscle tissues and homogenized it in ice-cold conditions. Following homogenization, purification was carried out by differential velocity centrifugation, equilibrium density gradient centrifugation, and finally, you have collected the fractions. But you have failed to detect any mitochondrial marker enzyme citrate synthase activity in the fractions. When you visualized the samples under the microscopes, you observed broken organelle debris due to severe proteolytic degradation. Which of the following statement may justify the cause of this result?

You were working with mouse mitochondrial proteins, and as a routine, you have isolated muscle tissues and homogenized it in ice-cold conditions. Following homogenization, purification was carried out by differential velocity centrifugation, equilibrium density gradient centrifugation, and finally, you have collected the fractions. But you have failed to detect any mitochondrial marker enzyme citrate synthase activity in the fractions. When you visualized the samples under the microscopes, you observed broken organelle debris due to severe proteolytic degradation. Which of the following statement may justify the cause of this result?

Name: You were working with mousemitochondrial proteins, and as a routine,y
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Description: You were working with mousemitochondrial proteins, and as a routine,you have isolated muscle tissues andhomogenized it in ice-cold conditions.Following homogenization, purificationwas carried out by differential velocitycentrifugation, equilibrium d

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter18: Glycolysis

Section: Chapter Questions

Problem 25P: Using the ActiveModel for phosphofructokinase (Trypanosoma), describe the difference between the...

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