is bradford assay using dye Coomassie Brilliant Blue G-250 a different method from using a spectrophotometer ? if so how is it different to determine protein concentration?

(b) Both laboratories used 10 micrograms of protein each in their kinetic assays. Protein concentrations weredetermined by the Bradford protein assay. Assay conditions employed in the two labs (pH, temperature,etc.) were also identical. What would be the most plausible cause for the discrepancy in the Vmax valuesfor the compound I? Explain.Recall that the Bradford assay measures total protein amounts in sample solution based on complexformation between a dye and proteins. Also, the assay solution used in both labs does not contain anyinhibitors.

For tha analysis of a specific drug present in different pharmaceutical preparations, explain when is more appropriate to use absorbtion based assay and when is more appropriate to use a fluorescence based assay.

Droplet based single cell DNA sequencing uses a microfluidic platform which generates droplets encapsulating individual cells. A micrograph of such a device [Zilionis et al, Nature Protocols 2016] is presented in Figure Q3. The channels are 25 μm deep. (a) (b) Oil Figure Q3. Micrograph of the microfluidic droplet device. Scale bars are 100 µm. Adapted from Zilionis et al, Nature Protocols 2016. Red arrowheads show individual cells, and black arrows indicate flow direction. (c) RT/lysis reagents Cells Barcoding hydrogel beads A company wants to explore the design of a prototype of the device made from PDMS. Describe a suitable fabrication process, explaining the rationale behind each individual step. Schematics can be drawn to illustrate your response. You may want to refer to the datasheets at the end of the script. Testing of the prototype was successful and the company wants to explore the feasibility of manufacturing the device from glass. Describe the fabrication process for this…

Why is it not necessary to dilute your protein samples with buffer in a bradford protein assay experiment?

What was studied or investigated in the paper? Who would be affected by this? https://opus.lib.uts.edu.au/bitstream/10453/31887/1/2012007630OK.pdf

how does polyacrylamide gel electrophoresis with SDS separate proteins ?

There are three names that often get used when discussing the Lowry assay. Those names are: Lowry, Folin-Ciocalteu, and Biuret. Are those names interchangeable when discussing the assay or do those names describe the same thing(s)? Significant differences? What is the relationship between those names?

Which of the following plates could represent the results of the transformation experiment with PGLO when plated on LB/Amp, if the experiment was successful? Refer to the letters on top of the image. Ignore the letters at the bottom. A B OB O none of the choices are correct