you were given an unknown peptide. Explain in detail how you would determine the amino acid sequence of this peptide by means of Edman degradation. (You can do this by showing how you would determine the N-terminal amino acid of the peptide shown below in figure 2 with Edman degradation.

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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you were given an unknown peptide. Explain in detail how you would determine the amino acid sequence of this peptide by means of Edman degradation. (You can do this by showing how you would determine the N-terminal amino acid of the peptide shown below in figure 2 with Edman degradation.

CH3
(S)
H2N.
(S)
N (R)_C.
'N'
N.
HO
H3C
CH3
`OH
|
Transcribed Image Text:CH3 (S) H2N. (S) N (R)_C. 'N' N. HO H3C CH3 `OH |
Expert Solution
Step 1

The amino acids that form the protein are mostly L forms. The carboxyl group of amino acids in the first position forms an amide bond with the amino group of the second amino acid.

The N terminal of the peptide is the side with a free amino group and the C terminal of the peptide is the side with the free carboxyl group. The peptides are synthesized in the translation from N to C terminal. Amino acid sequencing is the method by which, the order of amino acids forming the peptide chain is determined. 

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