1 0.8 0.6 Mutant y 0.4 Wild-Type 0.2 40 60 80 [ligand] 20

Principles Of Radiographic Imaging: An Art And A Science
6th Edition
ISBN:9781337711067
Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Chapter23: Technical Considerations In Digital Imaging
Section: Chapter Questions
Problem 6RQ
icon
Related questions
Question
You are working with a protein that is known to form an functioning complex of three identical polypeptide subunits, each with a binding site for a ligand of interest. You are studying an inherited mutation in the protein that changes one Asp residue to a Leu residue in each subunit. A comparison of the binding curves for the normal (wild-type) and mutant versions of the complex are shown below. Suggest a mechanism for the effect that this mutation has on the structure of the complex.   
 
1
0.8
0.6
Mutant
y
0.4
Wild-Type
0.2
20
40
60
80
[ligand]
Transcribed Image Text:1 0.8 0.6 Mutant y 0.4 Wild-Type 0.2 20 40 60 80 [ligand]
Expert Solution
Step 1

According to the given information, the wild type protein structure has aspartate in its primary structure. This aspartate is changed to leucine inthe mutant form of protein.

These wild type and mutant proteins differ in their binding affinities with ligand which is shown in binding curves of wild type and mutant proteins.

trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

Blurred answer
Recommended textbooks for you
Principles Of Radiographic Imaging: An Art And A …
Principles Of Radiographic Imaging: An Art And A …
Health & Nutrition
ISBN:
9781337711067
Author:
Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:
Cengage Learning
Essentials of Pharmacology for Health Professions
Essentials of Pharmacology for Health Professions
Nursing
ISBN:
9781305441620
Author:
WOODROW
Publisher:
Cengage