Suppose that there is a protein consisting of two polypeptide chains with the given sequences in the picture.   Before performing Edman sequencing method, it is required to identify the protein's amino acid composition first. Explain why it is required to do the said step first before doing Edman sequencing method.

You have been given an unknown low weight polypeptide to sequence. Luckily, you have a plentiful supply. You are given a limited amount of Edmond's reagent-just enough to perform 1 restricted digestion for every fragment that you generate but not for complete digestion of every fragment. You have the following enzymes and reagents available to do your digestion analysis: Chymotrypsin, V8, HCI (1M), Cyanogen Bromide (CnBr) and 50mM of ammonium bicarbonate. You are only given enough Carboxypeptidase for one fragment analysis. You fortunately have a plentiful supply of ion chromatography columns, eluting buffers, and SDS-PAGE gels to help you separate individual amino acids for analysis and fragment size (length) determination. And you have a really good spec and an HPLC. From the information given below, you will need to construct the sequence of your peptide! You also have the following information: 1. Digestion of the intact polypeptide with 1M HCl will liberate all amino acids (you…

Give typing answer with explanation and conclusion to all parts Maxim-Gilbert and Sanger Sequencing are two different methods used to sequence DNA. Describe the general techniques of Maxim-Gilbert and Sanger DNA Sequencing. List the advantages and disadvantages of each.

From the results of your BLAST search you can link to the GENE entry for one of your top hits. This link is located under the “Related Information” heading at the right hand side of each displayed alignment (i.e. scroll down to the“Alignments” section). Q1: How many exons and introns are annotated for this gene?  Q2: What is the function of the encoded protein?

You wish to align a 300 bp nucleotide sequence to a genome. Which of the following pairwise sequence alignment algorithms is BEST suited for this task? Global alignment or Local alignment? Please explain or elaborate why you chose this alignment algorithm.

Explain why a higher agarose concentration is preferred when seperating smaller DNA molecules. Include mathematical analysis in your answer.

how would you identify whether or not complete resolution of the three amino acids has been achieved

When the cDNA was sequenced by the Sanger method utilizing ddCTP, the following products were obtained: Tetranucleotide Hexanucleotide Nonanucleotide Decanucleotide Dodenucleotide Octadecanucleotide Nonadecanucleotide 21-nucleotide 6c. What is the sequence of the bases in the mRNA coding for the peptide above? The

Please explain how to use the global alignment algorithm: Needleman-Wunsch. Use the match 3, mismatch -3, linear gap penalty -3. Protein sequence 1: PHWG Protein sequence 2: HWEG

Transcriptome analysis involves two separate methodologies: gene expression and RNA seq analyses. The 10 items below are a scrambled listing of the steps used in the two procedures. Identify the steps involved in RNA seq from the list below. Use the numbers in the list to refer to each step. Once the steps for RNA seq have been identified, write the steps in the order in which they are performed during the experiment.   (1) DNA sequencing (2) Allow for hybridization and wash excess cRNA. (3) Mix labeled cRNA with array chip. (4) PCR amplification (5) Measure fluorescence intensity to determine abundance of transcripts. (6) Add labeled cRNA at each microarray location. (7) Map cDNA sequences to the genome of the organism to determine identity and abundance of transcripts. (8) mRNA isolation from cells (9) Prepare fluorescently labeled cRNA probes (10) cDNA synthesis