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You start with 100 units of protein activity and 100 grams of total protein. After the first centrifugation step, you have 25% less activity and 85% less total protein. What is your fold-purification of the target protein? 0.3-fold 3.4-fold 75-fold 5-fold 20-fold

You start with 100 units of protein activity and 100 grams of total protein. After the first centrifugation step, you have 25% less activity and 85% less total protein. What is your fold-purification of the target protein? 0.3-fold 3.4-fold 75-fold 5-fold 20-fold

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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**Protein Purification Calculation** You start with 100 units of protein activity and 100 grams of total protein. After the first centrifugation step, you have 25% less activity and 85% less total protein. What is your fold-purification of the target protein? - ⨀ 0.3-fold - ⨀ 3.4-fold - ⨀ 75-fold - ⨀ 5-fold - ⨀ 20-fold **Explanation for Calculation** 1. **Initial Protein Activity**: 100 units 2. **Initial Total Protein**: 100 grams **After Centrifugation:** - **Activity Loss**: 25% - Remaining Activity = 75% of 100 units = 75 units - **Protein Loss**: 85% - Remaining Protein = 15% of 100 grams = 15 grams **Fold-Purification Calculation:** \[ \text{Fold-Purification} = \frac{\text{Remaining Activity}}{\text{Remaining Protein}} = \frac{75 \text{ units}}{15 \text{ grams}} = 5\text{-fold} \] The correct answer is 5-fold.
Transcribed Image Text:**Protein Purification Calculation** You start with 100 units of protein activity and 100 grams of total protein. After the first centrifugation step, you have 25% less activity and 85% less total protein. What is your fold-purification of the target protein? - ⨀ 0.3-fold - ⨀ 3.4-fold - ⨀ 75-fold - ⨀ 5-fold - ⨀ 20-fold **Explanation for Calculation** 1. **Initial Protein Activity**: 100 units 2. **Initial Total Protein**: 100 grams **After Centrifugation:** - **Activity Loss**: 25% - Remaining Activity = 75% of 100 units = 75 units - **Protein Loss**: 85% - Remaining Protein = 15% of 100 grams = 15 grams **Fold-Purification Calculation:** \[ \text{Fold-Purification} = \frac{\text{Remaining Activity}}{\text{Remaining Protein}} = \frac{75 \text{ units}}{15 \text{ grams}} = 5\text{-fold} \] The correct answer is 5-fold.
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