You add 3.0 µg of plasmid DNA to a QIAprep spin miniprep purification column. You perform the binding in a binding buffer at pH 6.8. What portion (%) of the 3.0 µg of plasmid DNA would you expect to bind to the column in these conditions? What portion (%) of the bound plasmid DNA would you expect to elute using elution buffer at pH 8.5?    Make the following assumptions: • Solid phase silanol groups can have a very wide range of pKas. For the purpose of this problem, assume the silica gel functional group (silanol) in the DNA purification column matrix has a pKa of 7.5. • If a DNA molecule and column matrix functional groups have the same charge state, then that DNA molecule will be repelled and will not bind to the column. If they do not have the same charge, binding occurs.

For tha analysis of a specific drug present in different pharmaceutical preparations, explain when is more appropriate to use absorbtion based assay and when is more appropriate to use a fluorescence based assay.

Droplet based single cell DNA sequencing uses a microfluidic platform which generates droplets encapsulating individual cells. A micrograph of such a device [Zilionis et al, Nature Protocols 2016] is presented in Figure Q3. The channels are 25 μm deep. (a) (b) Oil Figure Q3. Micrograph of the microfluidic droplet device. Scale bars are 100 µm. Adapted from Zilionis et al, Nature Protocols 2016. Red arrowheads show individual cells, and black arrows indicate flow direction. (c) RT/lysis reagents Cells Barcoding hydrogel beads A company wants to explore the design of a prototype of the device made from PDMS. Describe a suitable fabrication process, explaining the rationale behind each individual step. Schematics can be drawn to illustrate your response. You may want to refer to the datasheets at the end of the script. Testing of the prototype was successful and the company wants to explore the feasibility of manufacturing the device from glass. Describe the fabrication process for this…

Select all that apply from the following to each transformation process : The following are White non-glowing colonies White glowing colonies Blue non-glowing colonies Blue glowing colonies None of the above The transformation are: a. LB + kanamycin plates that have been spread with the E. coli cells transformed with your ligations, what phenotype of the colonies do you expect to obtain . b. LB + kanamycin plates that have been spread with the E. coli cells transformed with water, what phenotype of the colonies do you expect to obtain. c. LB plates that have been spread with the E. coli cells transformed with your pHSG298, what phenotype of the colonies do you expect to obtain. d. LB + kanamycin plates that have been spread with the E. coli cells transformed with PHSG298, what phenotype of the colonies do you expect to obtain. e. LB plates that have been spread with the E. coli cells transformed with your ligations, what phenotype of the colonies do you expect to obtain. f. LB plates…