You receive purified mRNA encoding the peak protein of SARS Cov-2 as well as the plasmid pET32 (a +), how will you synthesize the cDNA of the peak protein and then clone in the pET32 (a +) vector

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You receive purified mRNA encoding the peak protein of SARS Cov-2 as well as the plasmid pET32 (a +), how will you synthesize the cDNA of the peak protein and then clone in the pET32 (a +) vector

Single Stranded RNA genome of SARS CoV-2
(~30kb length)
21,563
29,674
H 3'
266
13,468
5' F
Papain-like protease
(4,955-5,900)
Replicase
(13,442-16,236)
Helicase
(16,237-18,043)
Endoribonuclease
(19,621-20,658)
3CL-protease
(10,055-10,977)
Spike (S1&S2)
(21,563-25,384)
Transcribed Image Text:Single Stranded RNA genome of SARS CoV-2 (~30kb length) 21,563 29,674 H 3' 266 13,468 5' F Papain-like protease (4,955-5,900) Replicase (13,442-16,236) Helicase (16,237-18,043) Endoribonuclease (19,621-20,658) 3CL-protease (10,055-10,977) Spike (S1&S2) (21,563-25,384)
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