You obtained a sample of double-stranded DNA and transcribe mRNA from this DNA. You then analyse the base composition of each of the two DNA strands and the one mRNA strand, and get the following results. The numbers indicate percentage of each base in the strand: A G C T U strand 1 40.1 28.9 9.9 0.0 21.1 strand 2 21.5 9.5 29.9 39.1 0.0 strand 3 40.0 29.0 9.7 21.3 0.0 i) Which of these strands must be the mRNA? Explain. ii) Which one is the template strand for the mRNA? Explain.
Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
You obtained a sample of double-stranded DNA and transcribe mRNA from this DNA. You then analyse the base composition of each of the two DNA strands and the one mRNA strand, and get the following results. The numbers indicate percentage of each base in the strand:
A G C T U
strand 1 40.1 28.9 9.9 0.0 21.1
strand 2 21.5 9.5 29.9 39.1 0.0
strand 3 40.0 29.0 9.7 21.3 0.0
i) Which of these strands must be the mRNA? Explain.
ii) Which one is the template strand for the mRNA? Explain.
iii) What would be the likely sequence for the mRNA codons and the tRNA anticodons if the template DNA had the following sequence: 3' T A C A T A C G G T T A C G T 5'.
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